Dual-targeting of proteins and its role in coordinating organelle functions in plants. Innovative agricultural solutions in Australia's future will be built on understanding and manipulating the expression of groups of genes to influence whole plant phenotypes providing more robust plants and high value plant products. Plant energy organelles are central components in plant metabolism, their coordination by processes such as dual-targeting has potential to modify germination characteristics, ear ....Dual-targeting of proteins and its role in coordinating organelle functions in plants. Innovative agricultural solutions in Australia's future will be built on understanding and manipulating the expression of groups of genes to influence whole plant phenotypes providing more robust plants and high value plant products. Plant energy organelles are central components in plant metabolism, their coordination by processes such as dual-targeting has potential to modify germination characteristics, early seedling vigour, and stress tolerance. Studying energy organelles could generate valuable intellectual property to be applied within Australia's large plant-based industries and at the same time provide a rich intellectual environment for the training of research students and postdoctoral researchers.Read moreRead less
In touch with the environment: dissecting early tactile responses in plants. This project aims to identify the regulatory mechanisms that control touch-responses in plants. Although plants cannot relocate in the face of danger, they are able to sense mechanical manipulations from the environment. These could be caused by pathogens, herbivores, rain or even wind. This touch-responsiveness of plants is essential for pathogen resistance and for triggering architectural changes to overcome obstacles ....In touch with the environment: dissecting early tactile responses in plants. This project aims to identify the regulatory mechanisms that control touch-responses in plants. Although plants cannot relocate in the face of danger, they are able to sense mechanical manipulations from the environment. These could be caused by pathogens, herbivores, rain or even wind. This touch-responsiveness of plants is essential for pathogen resistance and for triggering architectural changes to overcome obstacles and prevent mechanical damage. Using a comprehensive tool set of genetics, genomics and proteomics, this project aims to identify the upstream regulators that control touch responses. Furthermore, it is expected to expand our understanding of the physiological impacts of touch-responses on growth and stress tolerance.Read moreRead less
Understanding the biological functions of the karrikin-responsive signaling system of plants in growth, development and responses to the environment. A new signalling system in plants, related to that of strigolactone hormones but evolutionarily more ancient and functionally distinct, has been discovered. It is defined by the Karrkin-Insensitive-2 (KAI2) protein discovered by its ability to confer responsiveness to karrikins from bushfires. The KAI2 system influences seed germination, and develo ....Understanding the biological functions of the karrikin-responsive signaling system of plants in growth, development and responses to the environment. A new signalling system in plants, related to that of strigolactone hormones but evolutionarily more ancient and functionally distinct, has been discovered. It is defined by the Karrkin-Insensitive-2 (KAI2) protein discovered by its ability to confer responsiveness to karrikins from bushfires. The KAI2 system influences seed germination, and development of seedlings, leaves and potentially roots. This project will use KAI2 mutants and transgenic plants to define the biological functions of KAI2 signalling, and its interactions with other signalling systems. New genes central to KAI2 signalling and responses will be identified for functional analysis. The research will reveal the significance of this new signalling system in plant biology. Read moreRead less
Ecophysiological limitations that affect water and carbon balance within large tree canopies: a comparative investigation. Supply of water to forest canopies is a major control of hydrological, atmospheric and biotic processes that impinge on groundwater stability, catchment yield, the fate of pollutants and plant productivity. Fundamental aspects of water transport and distribution within plant tissues remain obscured by conflicting experimental data and conflicting theoretical models that des ....Ecophysiological limitations that affect water and carbon balance within large tree canopies: a comparative investigation. Supply of water to forest canopies is a major control of hydrological, atmospheric and biotic processes that impinge on groundwater stability, catchment yield, the fate of pollutants and plant productivity. Fundamental aspects of water transport and distribution within plant tissues remain obscured by conflicting experimental data and conflicting theoretical models that describe physiological functioning.
Potential hydraulic constraints to the exchange of water and carbon between leaf and atmosphere require investigation before accurate models and informed decisions can be made with respect to the role of forests in biosphere-atmosphere processes. I will use large trees to investigate physiological and morphological determinants of hydraulic function and consider relationships between tree size, water requirements and water supply.
This project will foster comparative analyses of hydraulic functioning in large angiosperm and conifer species to elucidate universal principles that relate form to function and explain relationships between trees and their environment.
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Genome Approaches to Investigate Metabolic Coordination in Plant Cells. Metabolism of C and N in legume nodules requires interaction between the symbiotic bacteria and plant organelles, particularly metabolism in plastids and mitochondria. Fixed N is assimilated through the de novo synthesis of purines in both plastids and mitochondria. However, each of the nine pathway enzymes is encoded by a single gene, indicating each protein is targeted to both organelles. Purine metabolism will provide ....Genome Approaches to Investigate Metabolic Coordination in Plant Cells. Metabolism of C and N in legume nodules requires interaction between the symbiotic bacteria and plant organelles, particularly metabolism in plastids and mitochondria. Fixed N is assimilated through the de novo synthesis of purines in both plastids and mitochondria. However, each of the nine pathway enzymes is encoded by a single gene, indicating each protein is targeted to both organelles. Purine metabolism will provide a model to assess the more general occurrence of dual-targeted proteins in plants. The aim is to identify and eventually exploit the signalling mechanism(s) that mediate communication between plastids and mitochondria.Read moreRead less
Is transport of miRNAs essential for plant development? This project will provide knowledge of how a new class of biologically active molecule (micro RNA) regulates expression of genes at sites in the plant that are critical for growth and development. MicroRNAs are believed to influence the size and shape of plants, how rapidly they grow and how well they produce and fill seeds. These molecules are part of a group of bioactive signals that move throughout the plant, functioning like hormones bu ....Is transport of miRNAs essential for plant development? This project will provide knowledge of how a new class of biologically active molecule (micro RNA) regulates expression of genes at sites in the plant that are critical for growth and development. MicroRNAs are believed to influence the size and shape of plants, how rapidly they grow and how well they produce and fill seeds. These molecules are part of a group of bioactive signals that move throughout the plant, functioning like hormones but directly influencing how well critical genes work. Their exploitation holds great promise for manipulating plant performance and enhancing crop yields. Read moreRead less
How do sunflowers make protein drugs in their seeds? We recently discovered in sunflower the origin of a small protein ring that chemists have used for a decade to base designed drugs upon. This project aims to know how sunflowers make it so we may manipulate other plants to manufacture ring-based drugs.
Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on ....Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on some landscapes. Rushes are also highly sensitive to small increments in nutrients in disturbed environments and thus form a management priority relating to their use in rehabilitation of degraded landscapes, such as mine sites and wetland margins. Read moreRead less
Circular Plant Proteins with Pharmaceutical Applications. The proposed research will develop methods for using plants as protein production factories. Initially I will use plants to create engineered cyclotides that incorporate peptides with proven therapeutic activity against cancer and multiple sclerosis. Successful production of therapeutic proteins in plants will benefit Australians by making treatments for these and other diseases more accessible. It also has the potential for a major econo ....Circular Plant Proteins with Pharmaceutical Applications. The proposed research will develop methods for using plants as protein production factories. Initially I will use plants to create engineered cyclotides that incorporate peptides with proven therapeutic activity against cancer and multiple sclerosis. Successful production of therapeutic proteins in plants will benefit Australians by making treatments for these and other diseases more accessible. It also has the potential for a major economic benefit from the sales of Australian-based drugs. This proposal will also provide outstanding research training for graduate students in multidisciplinary methods that constitute state-of the-art structural and plant molecular biology.Read moreRead less
Ecology of fine root endophytes in native and agricultural ecosystems. This project aims to investigate the abundance, diversity and function of fine root endophytes in Australian native and agricultural ecosystems in response to recent genetic data proving they are taxonomically distinct from the well-studied arbuscular mycorrhizal fungi. The project aims to complete a comprehensive field survey, novel molecular analyses and innovative glasshouse experiments. Outcomes should include globally si ....Ecology of fine root endophytes in native and agricultural ecosystems. This project aims to investigate the abundance, diversity and function of fine root endophytes in Australian native and agricultural ecosystems in response to recent genetic data proving they are taxonomically distinct from the well-studied arbuscular mycorrhizal fungi. The project aims to complete a comprehensive field survey, novel molecular analyses and innovative glasshouse experiments. Outcomes should include globally significant insights into fine root endophytes and their role in plant growth. The project will strengthen capacity to predict impacts of global environmental change on ecosystem functions driven by the soil rhizosphere.Read moreRead less