Developing the basis for an quality control platform for human pluripotent stem cells and their differentiated derivatives. Biophotonic techniques based on spectroscopy have the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells produced for use in industry and regenerative medicine. This project is aimed at acquiring the fundamental scientific knowledge base required to bring this about.
Pushing the Boundaries of Multi-modal Biospectroscopic Microscopies. In order to understand the fundamentals of life processes, diseases, and their treatments, it is essential to probe fundamental changes in molecular processes in cells, tissues and whole organisms. Much of our understanding of these processes has involved the introduction of chemical probes for biospectroscopy, but these have inherent problems because the probe can often change the biochemistry that is being probed. This projec ....Pushing the Boundaries of Multi-modal Biospectroscopic Microscopies. In order to understand the fundamentals of life processes, diseases, and their treatments, it is essential to probe fundamental changes in molecular processes in cells, tissues and whole organisms. Much of our understanding of these processes has involved the introduction of chemical probes for biospectroscopy, but these have inherent problems because the probe can often change the biochemistry that is being probed. This project will push the boundaries of a variety of micro and nano "probe-free" microscopies to provide fundamental insights into these life processes, which could ultimately lead to improvements in the diagnosis, prevention and treatment of diseases.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100063
Funder
Australian Research Council
Funding Amount
$980,000.00
Summary
Focused ion beam microscope for trace element analysis and nanomachining. Focused ion beam microscope for trace element analysis and nanomachining:
This project aims to fill the critical gap in 3-D imaging and compositional characterisation of metals, functional materials, polymers, biomaterials, ceramics and minerals at micro- and nano-scales. Coupling of dual column focused ion beam microscopy with secondary ion mass spectroscopy analysis will is designed to overcome the long-standing limitat ....Focused ion beam microscope for trace element analysis and nanomachining. Focused ion beam microscope for trace element analysis and nanomachining:
This project aims to fill the critical gap in 3-D imaging and compositional characterisation of metals, functional materials, polymers, biomaterials, ceramics and minerals at micro- and nano-scales. Coupling of dual column focused ion beam microscopy with secondary ion mass spectroscopy analysis will is designed to overcome the long-standing limitation of light and trace element analysis in scanning electron microscopes. This facility would provide Australian researchers with a new capability of characterising light and trace elements using scanning electron microscopy. Along with the ability to characterise a diverse range of materials in 3-D, the new system would enable fabrication of functional nanoscale devices for nanotechnology, biomedical and energy applications. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE110100191
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
An advanced mass spectrometer for applications in phospho-proteomics, glycomics and top-down sequencing of proteins. This cutting-edge mass spectrometry facility will benefit the Hunter Valley research community comprising 100 researchers in this field. It will enable the researchers to enhance their research productivity in areas of national importance, including better understanding the etiology of disease states, reproductive health and the regulation of plant growth.
Single cell imaging of trace elements by laser ablation - inductively coupled plasma - mass spectrometry. The precise mechanism of how many diseases function on the cellular level is not well understood. Trace elements are important to normal cellular function and have the potential to cause significant damage if delicate levels are disturbed. This project will introduce a new, cost-effective alternative to the synchrotron for mapping of trace elements in single cells. This breakthrough science ....Single cell imaging of trace elements by laser ablation - inductively coupled plasma - mass spectrometry. The precise mechanism of how many diseases function on the cellular level is not well understood. Trace elements are important to normal cellular function and have the potential to cause significant damage if delicate levels are disturbed. This project will introduce a new, cost-effective alternative to the synchrotron for mapping of trace elements in single cells. This breakthrough science will transform a common analytical instrument into a powerful new tool for probing the cellular mechanisms of chronic illness. This frontier technology will help determine the role of trace metals in the development of neurodegenerative disease.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE200100053
Funder
Australian Research Council
Funding Amount
$482,055.00
Summary
Single Particle Elemental Analysis – Imaging Mass Cytometer Facility. This proposal aims to establish an Australian facility for next generation high resolution inductively coupled plasma time-of-flight mass spectrometry (icpTOF-MS), coupled with high speed laser ablation (LA) imaging. The facility, being the first in Australia, will allow for the entire elemental/isotopic make-up of individual particles and cells in suspension, or within a solid sample, to be performed simultaneously. This is p ....Single Particle Elemental Analysis – Imaging Mass Cytometer Facility. This proposal aims to establish an Australian facility for next generation high resolution inductively coupled plasma time-of-flight mass spectrometry (icpTOF-MS), coupled with high speed laser ablation (LA) imaging. The facility, being the first in Australia, will allow for the entire elemental/isotopic make-up of individual particles and cells in suspension, or within a solid sample, to be performed simultaneously. This is particularly significant when materials are rare, or not readily available. Expected outcomes include the generation of new knowledge and training in the industrial, biological, defence and environmental research sectors and the significant enhancement of Australia’s leadership in nanotechnology and elemental imaging.
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Formation, photochemistry and fate of gas-phase peroxyl radicals. This project aims to understand how peroxyl radical reactions modulate the composition of air. The gas-phase chemical reactions of organic peroxyl radicals contribute to air quality in clean and polluted environments. However, experimental observations of these reaction intermediates and the complex mechanisms governing their formation and fate are limited. This project will use mass spectrometry and laser-based methods to interro ....Formation, photochemistry and fate of gas-phase peroxyl radicals. This project aims to understand how peroxyl radical reactions modulate the composition of air. The gas-phase chemical reactions of organic peroxyl radicals contribute to air quality in clean and polluted environments. However, experimental observations of these reaction intermediates and the complex mechanisms governing their formation and fate are limited. This project will use mass spectrometry and laser-based methods to interrogate the chemical and photochemical reactions of peroxyl radicals in the gas phase. This project expects to understand the composition and dynamics of the troposphere and inform strategies to improve air quality.Read moreRead less
Microfluidic Separation Science: Innovative Technology for Characterising Complex Chemical Systems. At present there is a need for fast and detailed chemical analysis of complex samples, such as those important to biomedical diagnostics and forensic science. Innovative technology will be developed here in order to reduce analysis time whilst maintaining the integrity of the chemical information contained within the sample. This step change in separation science will directly aid biomedical diagn ....Microfluidic Separation Science: Innovative Technology for Characterising Complex Chemical Systems. At present there is a need for fast and detailed chemical analysis of complex samples, such as those important to biomedical diagnostics and forensic science. Innovative technology will be developed here in order to reduce analysis time whilst maintaining the integrity of the chemical information contained within the sample. This step change in separation science will directly aid biomedical diagnostics, forensic sample determination and industrial process monitoring through decreased analysis time with an increase in the chemical information gained. By performing chemical separations on a microfluidic scale a reduction in both the cost of analysis and impact of solvent waste on the environment will be achieved.Read moreRead less
Identification and isolation of new pharmaceutical opiate analogues: today's problems tomorrow's solutions. This project will identify and characterise the impurity profiles across opiate alkaloid production, which will enhance the development of manufacturing processes through the reduction of problem impurities and provide potential new drug substances from the isolation of other opiate derived concomitant species.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE200100043
Funder
Australian Research Council
Funding Amount
$389,000.00
Summary
Rapid Molecular (Bio)material Imaging by Infrared and Raman Microscopies. This project aims to undertake fast probe-free biochemical/chemical imaging of heterogeneity within cells and materials surfaces with new infrared and Raman imaging. It will generate new fundamental knowledge on: cell heterogeneity and dynamic processes; technologies for optimising cell printing; understanding toxicity of microplastics; and protocols for measuring materials of technological relevance. Expected outcomes inc ....Rapid Molecular (Bio)material Imaging by Infrared and Raman Microscopies. This project aims to undertake fast probe-free biochemical/chemical imaging of heterogeneity within cells and materials surfaces with new infrared and Raman imaging. It will generate new fundamental knowledge on: cell heterogeneity and dynamic processes; technologies for optimising cell printing; understanding toxicity of microplastics; and protocols for measuring materials of technological relevance. Expected outcomes include: interdisciplinary collaborations in new protocols for in-vitro drug development; cell printing technologies; environmental impacts of microplastics; and materials design. Expected benefits include innovative approaches to early stage drug design; improved environmental controls and advances in innovative materials.Read moreRead less