Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plan ....Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plants coordinate and adapt to changing water and salt conditions, addressing a missing link in how ions and water move in and out of plant vacuoles. Benefits include an expanded, innovative range of targets for plant breeding programs to improve plant productivity in our changing climate.Read moreRead less
Controlling accumulation of elements in the shoots of higher plants by manipulating processes in specific cell types in the roots. This project will provide novel, fundamental understanding of the processes controlling accumulation of elements in the shoots of plants. As such, it will impact on our understanding of processes relevant to stress tolerance, plant nutrition, human nutrition and the removal of toxic metals from soils by plants. These are all areas of great importance to Australian ag ....Controlling accumulation of elements in the shoots of higher plants by manipulating processes in specific cell types in the roots. This project will provide novel, fundamental understanding of the processes controlling accumulation of elements in the shoots of plants. As such, it will impact on our understanding of processes relevant to stress tolerance, plant nutrition, human nutrition and the removal of toxic metals from soils by plants. These are all areas of great importance to Australian agriculture, environmental sustainability and human health. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this ....Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Membrane transporters in oxidative stress signalling and tolerance in plants. Oxidative stress imposed by salinity and drought severely limits agricultural crop production, resulting in multibillion dollar losses to farmers. Australia is one of the driest continents, with a significant proportion of arable land affected by salinity. Thus, developing salt- and drought tolerant species is critical to minimise the impact of these stresses on crop production. This project will reveal specific ionic ....Membrane transporters in oxidative stress signalling and tolerance in plants. Oxidative stress imposed by salinity and drought severely limits agricultural crop production, resulting in multibillion dollar losses to farmers. Australia is one of the driest continents, with a significant proportion of arable land affected by salinity. Thus, developing salt- and drought tolerant species is critical to minimise the impact of these stresses on crop production. This project will reveal specific ionic mechanisms mediating reactive oxygen species signalling and tolerance in plants. This will help achieve the above goal by providing plant breeders with vital information on key genes controlling oxidative stress tolerance in plants. Read moreRead less
Mitochondrial Biogenesis and Signalling in Plants . This proposal aims to define the mechanisms of how mitochondrial growth and stress signalling interact and are regulated. Mitochondria are central machines in cells that use energy obtained through photosynthesis to drive growth and also play an important role in sensing and responding to non-optimal environmental growth conditions. As mitochondrial growth and stress signalling are antagonistic, growth is retarded when stress signalling is acti ....Mitochondrial Biogenesis and Signalling in Plants . This proposal aims to define the mechanisms of how mitochondrial growth and stress signalling interact and are regulated. Mitochondria are central machines in cells that use energy obtained through photosynthesis to drive growth and also play an important role in sensing and responding to non-optimal environmental growth conditions. As mitochondrial growth and stress signalling are antagonistic, growth is retarded when stress signalling is activated. Thus, the outcomes will be new knowledge and understanding of how plants balance growth and stress responses. This benefit of this knowledge and understanding is that it can be used to pursue novel avenues to optimise crop performance in changing and adverse environments.Read moreRead less
Coping with flooding: nutrient transport in oxygen-deprived roots. Flooding damages plants by reducing oxygen supply to roots. The project will study effects of low oxygen on nutrient transport by roots. Understanding root functioning during low oxygen will enhance knowledge of plant acclimation to soil water logging. The project will contribute to the National Goal of 'Responding to Climate Change and Variability'.
Clocks in crops: exploring the role of circadian rhythms in crop adaptation. The project aims to analyse how the circadian clock provides adaptive advantages in legumes and cereals, two major crop groups of global importance. The project expects to produce new fundamental knowledge about how the clock in these crops coordinates their development, physiology, and metabolism in response to environmental cues. The anticipated outcome is a better understanding of key similarities and differences in ....Clocks in crops: exploring the role of circadian rhythms in crop adaptation. The project aims to analyse how the circadian clock provides adaptive advantages in legumes and cereals, two major crop groups of global importance. The project expects to produce new fundamental knowledge about how the clock in these crops coordinates their development, physiology, and metabolism in response to environmental cues. The anticipated outcome is a better understanding of key similarities and differences in clock functions in different species. Another is knowledge of how the ancient clock gene variants still in use today may limit the current or future performance of those crops. This will provide significant benefits, such as to improve productivity of crop plants in diverse and changing environments.Read moreRead less
Cellulose-based composites as models for primary plant cell walls of cereals and grasses. Cereals and grasses are the lynchpins of the Australian Agri-Food industry. Cell walls provide shape, form and barrier properties to the plant and are the basis for both post-harvest mechanical properties and direct nutritional benefits. There is as yet no validated model for the molecular assembly, architecture and mechanical behaviour of cereal/grass cell walls. This project aims to derive such a model, s ....Cellulose-based composites as models for primary plant cell walls of cereals and grasses. Cereals and grasses are the lynchpins of the Australian Agri-Food industry. Cell walls provide shape, form and barrier properties to the plant and are the basis for both post-harvest mechanical properties and direct nutritional benefits. There is as yet no validated model for the molecular assembly, architecture and mechanical behaviour of cereal/grass cell walls. This project aims to derive such a model, so that predictions can be made concerning the effects of tailoring either plant composition (e.g. at the gene level) or post-harvest treatment in order to achieve desired plant or food properties. The findings will also be relevant to understanding how individual features of cell walls affect digestibility and nutritional properties.Read moreRead less
Preparation of Photo-Affinity Molecular Probes for the Identification of Gibberellin Receptors. Bioactive gibberellins affect numerous processes during plant growth and development, including seed germination, leaf expansion, stem elongation, flowering and fruit development. However, only very limited information is available regarding their mode of action at the molecular level. The central aim of the project is to prepare a family of photo-affinity molecular probes based on the gibberellin m ....Preparation of Photo-Affinity Molecular Probes for the Identification of Gibberellin Receptors. Bioactive gibberellins affect numerous processes during plant growth and development, including seed germination, leaf expansion, stem elongation, flowering and fruit development. However, only very limited information is available regarding their mode of action at the molecular level. The central aim of the project is to prepare a family of photo-affinity molecular probes based on the gibberellin molecule that will be designed to provide critical information on the location and structure of gibberellin receptors. Screening of the probes for potential effectiveness will be determined initially by the measurement of alpha-amylase produced in a standard barley aleurone assay.Read moreRead less
Investigating a novel signalling pathway for crop improvement. This project will dissect a newly identified signalling pathway in plants that regulates plant water use and carbon gain. It will deploy multiple techniques, including novel biosensors, to understand the links between the metabolism of plants and their environmental responses. The project will build partnerships with scientists at leading international institutions for enhanced outcomes, including access to specialised equipment and ....Investigating a novel signalling pathway for crop improvement. This project will dissect a newly identified signalling pathway in plants that regulates plant water use and carbon gain. It will deploy multiple techniques, including novel biosensors, to understand the links between the metabolism of plants and their environmental responses. The project will build partnerships with scientists at leading international institutions for enhanced outcomes, including access to specialised equipment and upskilling of our scientists. The generation of barley with the latest gene editing techniques aims to produce a non-GM crop with the potential for enhanced root C sequestration, lower water use and improved yield, three key goals for agricultural sustainability in the face of a drying Australian climate.Read moreRead less