Neurotrophic factors for pelvic autonomic neurons: the role of neurturin. This project is about pelvic parasympathetic neurons, which are responsible for involuntary activities such as bladder voiding and penile erection. We are interested in the neurotrophic factors that determine survival of these neurons during early mammalian development and keep them healthy in adults. Little is known about ?parasympathetic neurotrophic factors? in general. However we have recently discovered that the prote ....Neurotrophic factors for pelvic autonomic neurons: the role of neurturin. This project is about pelvic parasympathetic neurons, which are responsible for involuntary activities such as bladder voiding and penile erection. We are interested in the neurotrophic factors that determine survival of these neurons during early mammalian development and keep them healthy in adults. Little is known about ?parasympathetic neurotrophic factors? in general. However we have recently discovered that the protein neurturin is very important in the pelvic parasympathetic system. We will determine exactly how neurturin affects pelvic neurons and how it interacts with other neurotrophic factors. Our results will fill a major gap in our knowledge of fundamental neurobiology.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0346903
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
LC-MS system for Faculty of Sciences, U.N.E. LC-MS is a technique for investigating the composition of complex mixtures and identifying its components. This instrumentation will be utilised at UNE in a diverse range of analytical investigations to further the research aims of scientists in chemistry, biological sciences, archaeology, physics, environmental sciences, marine science as well as biological researchers in specialised units based at UNE. The extraordinarily versatile capacity of this ....LC-MS system for Faculty of Sciences, U.N.E. LC-MS is a technique for investigating the composition of complex mixtures and identifying its components. This instrumentation will be utilised at UNE in a diverse range of analytical investigations to further the research aims of scientists in chemistry, biological sciences, archaeology, physics, environmental sciences, marine science as well as biological researchers in specialised units based at UNE. The extraordinarily versatile capacity of this technique for analysis and identification of organic substances, from small molecules through to large proteins and complex plant and animal metabolites, will provide impetus for existing research and stimulus for new research directions.Read moreRead less
Genetics and evolution of Shigella O antigens. We use genome scale sequencing techniques to sequence 26 O-antigen gene clusters from Shigella. With the seven already known, this will give sequences for every O-antigen of Shigella. This will be the first time that such set is fully sequenced. Shigella are human specific pathogens, have emerged with the evolution of humans. O-antigens are important for their life and pathogenicity. This project will greatly extend our knowledge of the genetic basi ....Genetics and evolution of Shigella O antigens. We use genome scale sequencing techniques to sequence 26 O-antigen gene clusters from Shigella. With the seven already known, this will give sequences for every O-antigen of Shigella. This will be the first time that such set is fully sequenced. Shigella are human specific pathogens, have emerged with the evolution of humans. O-antigens are important for their life and pathogenicity. This project will greatly extend our knowledge of the genetic basis and evolution of this important polymorphism. O-antigens are used for typing Shigella and also elicit strong immunity. The molecular data will help establish DNA based typing and vaccine development.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454052
Funder
Australian Research Council
Funding Amount
$733,595.00
Summary
Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans ....Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans of a network of institutions from Queensland and New South Wales and their collaborators. Access to such instrumentation is critical to high level achievement in proteomics, a key platform technology for National Research Priorities relating to Frontier Technologies. No comparable instrument currently exists in Australia.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989105
Funder
Australian Research Council
Funding Amount
$495,000.00
Summary
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national i ....An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national importance. These include understanding the impact of the environment on plant and animal development, pest animal control, development of new biotechnology tools, new drugs and new methods for the detection of narcotics and explosives.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561041
Funder
Australian Research Council
Funding Amount
$347,358.00
Summary
A New Generation Biosensor and Fluorescence Facility for Proteomics. The complete DNA sequence (the genome) is now known for many organisms and advances are being made to identify the complement of messenger RNA (the transcriptome) and the resultant collection of proteins (the proteome). The genome is largely fixed while the transcriptome and proteome differ between cell types in an organism and constantly vary to adapt the cell to changing conditions. The mediators of these variations are prote ....A New Generation Biosensor and Fluorescence Facility for Proteomics. The complete DNA sequence (the genome) is now known for many organisms and advances are being made to identify the complement of messenger RNA (the transcriptome) and the resultant collection of proteins (the proteome). The genome is largely fixed while the transcriptome and proteome differ between cell types in an organism and constantly vary to adapt the cell to changing conditions. The mediators of these variations are proteins, interacting with each other and with signal molecules. The next frontier in molecular biology is to identify and quantify these protein interactions. Our two institutions have a very large cohort of biologists whose research on proteins would be greatly facilitated by the Biacore 3000 and the ISS K2.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0883032
Funder
Australian Research Council
Funding Amount
$1,300,000.00
Summary
800 MHz NMR spectrometer for biomolecular structure-function analysis. An understanding of how organisms function at the molecular level is central to developing the ability to fight many diseases in a rational way. This equipment will provide the capability for many different laboratories around NSW and the ACT to advance our knowledge at this fundamental level, primarily by examining the structures and functions of biomolecules such as proteins.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0882682
Funder
Australian Research Council
Funding Amount
$100,000.00
Summary
The Australasian Pollen and Spore Atlas. The results generated in this project will enhance Australian research capabilities across multiple disciplines by providing access to key knowledge of pollen and spores in our region. A unified approach to the archiving, presentation and accessibility to existing and evolving databases will provide a considerably improved context for identification and knowledge pooling of any given pollen or spore type. This will create a nexus for novel interactions be ....The Australasian Pollen and Spore Atlas. The results generated in this project will enhance Australian research capabilities across multiple disciplines by providing access to key knowledge of pollen and spores in our region. A unified approach to the archiving, presentation and accessibility to existing and evolving databases will provide a considerably improved context for identification and knowledge pooling of any given pollen or spore type. This will create a nexus for novel interactions between researchers and end users of these data from within and beyond Australia's borders.Read moreRead less
Sociogenomics of honeybees. From genes to society. This project will show how complex social behaviour of honeybees like dance communication and thermoregulation is genetically controlled. The complete sequence of the honeybee genome will be published in 2003, and a gene chip based on it will be created shortly afterwards. These new technologies will make our project technically feasible. By backcrossing, workers with different genetic tendencies to do a task can be generated within an otherwi ....Sociogenomics of honeybees. From genes to society. This project will show how complex social behaviour of honeybees like dance communication and thermoregulation is genetically controlled. The complete sequence of the honeybee genome will be published in 2003, and a gene chip based on it will be created shortly afterwards. These new technologies will make our project technically feasible. By backcrossing, workers with different genetic tendencies to do a task can be generated within an otherwise uniform background. Age-matched workers that perform a behaviour like thermoregulation can be compared to sisters that do not, and the genes that are switched on in the two groups compared.Read moreRead less
Proteomic analysis of subcellular changes during apoptosis. This project aims to use a novel proteomic approach to examine mechanisms of apoptosis at the level of the plasma membrane, mitochondrion, nucleus and cytosol, screening protein extracts of cell organelles by the new technique of SELDI-TOF mass spectrometry in which proteins are adsorbed onto activated chips. This will provide protein mass profiles characteristic of various stages of apoptosis, and will allow identification of proteins ....Proteomic analysis of subcellular changes during apoptosis. This project aims to use a novel proteomic approach to examine mechanisms of apoptosis at the level of the plasma membrane, mitochondrion, nucleus and cytosol, screening protein extracts of cell organelles by the new technique of SELDI-TOF mass spectrometry in which proteins are adsorbed onto activated chips. This will provide protein mass profiles characteristic of various stages of apoptosis, and will allow identification of proteins of interest by conventional proteomic methods. The establishment of SELDI-MS as a viable tool for cell proteomics would open new opportunities to understand a broad range of cellular functions at the level of protein expression.Read moreRead less