Aptamer imaging mass spectrometry for biomarker quantification. This project aims to develop novel methods for quantifying bio-markers in histological specimens using aptamers, lanthanide visualising tags and laser ablation plasma mass spectrometry. Aptamers are short synthetic strands of nucleic acid with complex three dimensional structures that bind targets with exquisite specificity. This technology meets a major gap in current imaging modalities, has wide application to basic biology and di ....Aptamer imaging mass spectrometry for biomarker quantification. This project aims to develop novel methods for quantifying bio-markers in histological specimens using aptamers, lanthanide visualising tags and laser ablation plasma mass spectrometry. Aptamers are short synthetic strands of nucleic acid with complex three dimensional structures that bind targets with exquisite specificity. This technology meets a major gap in current imaging modalities, has wide application to basic biology and diagnostics, and will eliminate the subjective interpretation of immunohistochemical stains.Read moreRead less
Photodissociation mass spectrometry for lipidome analysis. This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisatio ....Photodissociation mass spectrometry for lipidome analysis. This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisation of the, potentially, tens of thousands of individual molecular lipid species that may be present within a sample of interest. This project will develop and optimise novel, ultraviolet photodissociation-tandem mass spectrometry methods which will be integrated within an automated lipidome analysis workflow, to enable comprehensive global lipidome profiling and to reveal the structural diversity of lipids involved in fundamental cellular signalling processes.Read moreRead less
Developing next-generation mass spectrometry imaging with isomer resolution. Mass spectrometry imaging (MSI) is a rapidly emerging technology for mapping molecular distributions within biological samples. This project will bring together market-leading MSI instrumentation from the industry partner Waters Corporation with unique technologies developed at QUT and UOW to develop an integrated MSI-platform capable of achieving high mass- and spatial-resolution, as well as discrimination of lipid iso ....Developing next-generation mass spectrometry imaging with isomer resolution. Mass spectrometry imaging (MSI) is a rapidly emerging technology for mapping molecular distributions within biological samples. This project will bring together market-leading MSI instrumentation from the industry partner Waters Corporation with unique technologies developed at QUT and UOW to develop an integrated MSI-platform capable of achieving high mass- and spatial-resolution, as well as discrimination of lipid isomers. Resolution of lipid isomers using this instrumentation will afford researchers a first glimpse of isomer-resolved images that will be used to visualise tissue-specific changes resulting from underlying chemical, physical or metabolic processes; changes that are currently invisible to contemporary imaging technologies.Read moreRead less
Pluses and minuses of lipid mass spectrometry. This project aims to investigate the structural diversity of lipids. Lipids are among the most structurally diverse of all the biomolecules and thus deciphering their many functions requires bio-analytical technologies capable of uniquely identifying and quantifying individual molecules in a milieu of many thousands of analogues. Mass spectrometry is the pre-eminent technique for contemporary lipid analysis but is challenged by the preference of cer ....Pluses and minuses of lipid mass spectrometry. This project aims to investigate the structural diversity of lipids. Lipids are among the most structurally diverse of all the biomolecules and thus deciphering their many functions requires bio-analytical technologies capable of uniquely identifying and quantifying individual molecules in a milieu of many thousands of analogues. Mass spectrometry is the pre-eminent technique for contemporary lipid analysis but is challenged by the preference of certain lipids to ionise with a polarity that affords sensitive detection but does not permit detailed structure elucidation. This project will develop advanced instrumentation capable of on-demand polarity switching of ionised lipids such that the detection and interrogation of molecular structure can take place in the optimal charge state.Read moreRead less
Industrial Transformation Training Centres - Grant ID: IC210100040
Funder
Australian Research Council
Funding Amount
$4,997,903.00
Summary
ARC Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB). The Centre for Facilitated Advancement of Australia's Bioactives (FAAB) will transform the rapidly growing bioactive ingredients sector. It will apply advanced analytical methods to molecular characterisation of bioactive products derived from foods, food-waste, and cell-based biotechnologies. FAAB will determine modes of action for bioactives with potential lifestyle and nutritional benefits allowing for evidence- ....ARC Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB). The Centre for Facilitated Advancement of Australia's Bioactives (FAAB) will transform the rapidly growing bioactive ingredients sector. It will apply advanced analytical methods to molecular characterisation of bioactive products derived from foods, food-waste, and cell-based biotechnologies. FAAB will determine modes of action for bioactives with potential lifestyle and nutritional benefits allowing for evidence-informed decision-making, and regulatory framework development. FAAB graduates will lead and deliver future national self-reliance to the Australian bioactives sector, increasing diversification and international competitiveness and development of regulation in a growing market. Read moreRead less
Connecting soil nitrogen and plant uptake for greener agriculture. This project will use synthetic organic chemistry, biochemistry, root and rhizosphere biology and rhizosphere modelling to establish detailed mechanistic knowledge of the nitrogen (N) transport and uptake processes at the soil-root interface to develop new, efficient urease and nitrification inhibitors for reliable provision of N to the plant/root system. The reduction of excessive N fertilisation has significant environmental be ....Connecting soil nitrogen and plant uptake for greener agriculture. This project will use synthetic organic chemistry, biochemistry, root and rhizosphere biology and rhizosphere modelling to establish detailed mechanistic knowledge of the nitrogen (N) transport and uptake processes at the soil-root interface to develop new, efficient urease and nitrification inhibitors for reliable provision of N to the plant/root system. The reduction of excessive N fertilisation has significant environmental benefits by reducing greenhouse gas emissions and water pollution. This project will lead to a breakthrough for the triple challenge of food security, environmental degradation and climate change, while improving plant productivity and increasing the profitability of agriculture through lower fertiliser costs.Read moreRead less
Understanding the mechanisms of class B GPCR-transducer coupling. Current effort in developing drugs targeting G protein-coupled receptors (GPCRs) often result in low success rate due to the lack of understanding of the complexity and the spatiotemporal control of receptor function. The research program aims to understand the molecular mechanisms of receptor/transducer selectivity. The proposal integrated multi-disciplinary approaches to provide a deeper understanding of how the receptor is acti ....Understanding the mechanisms of class B GPCR-transducer coupling. Current effort in developing drugs targeting G protein-coupled receptors (GPCRs) often result in low success rate due to the lack of understanding of the complexity and the spatiotemporal control of receptor function. The research program aims to understand the molecular mechanisms of receptor/transducer selectivity. The proposal integrated multi-disciplinary approaches to provide a deeper understanding of how the receptor is activated responding to different ligands. The anticipated outcome including an enhanced capacity for understanding the fundamental biology, a stronger national and international collaborations. This will provide significant benefits including expanded basic knowledge and improvements in drug development efficiency. Read moreRead less
Multi-functional probes for global analysis of proteome stress in cells. This project aims to create a suite of multi-functional chemical probes to identify damaged proteins that undergo unfolding or specific modifications in cells under stress. These probes will not only generate fluorescence responses to reflect on protein quality control capacity but allow associated proteins and their networks to be identified in complex cellular environments, which is difficult to achieve by current methods ....Multi-functional probes for global analysis of proteome stress in cells. This project aims to create a suite of multi-functional chemical probes to identify damaged proteins that undergo unfolding or specific modifications in cells under stress. These probes will not only generate fluorescence responses to reflect on protein quality control capacity but allow associated proteins and their networks to be identified in complex cellular environments, which is difficult to achieve by current methods. The expected outcome is to deliver new methodology for a comprehensive understanding of the correlation between quality control machinery, stress responses and cell functions. This should provide significant benefits, including contributing to fundamental knowledge on the molecular causes of neurodegenerative diseases.Read moreRead less
Integrated Nanoplatform for Multiomics Analysis of Cell-to-Cell Interaction. This project aims to develop an integrated nanoplatform for analysis of exosomes produced by host-pathogen interaction at the single cell level. This will be accomplished by engineering an innovative device involving plasmonic nanoparticles to probe exosomes molecular profiles over time. The intended outcome is a generic and robust platform for detailed molecular analysis of the consequences of cell-to-cell interactions ....Integrated Nanoplatform for Multiomics Analysis of Cell-to-Cell Interaction. This project aims to develop an integrated nanoplatform for analysis of exosomes produced by host-pathogen interaction at the single cell level. This will be accomplished by engineering an innovative device involving plasmonic nanoparticles to probe exosomes molecular profiles over time. The intended outcome is a generic and robust platform for detailed molecular analysis of the consequences of cell-to-cell interactions. Single cell scale will greatly improve detection accuracy for heterogeneous cell populations. Benefits will include new knowledge of cell-to-cell communication and intellectual property in manufacturing, which will foster collaborations across institutions and Australian industry by providing new technological solutions.Read moreRead less