A multiplex microscope platform to define molecular events in fluid systems. This project aims to develop a novel microscopy platform that will enable the visualisation and quantification of molecular events occurring under fluid shear stress. The project will generate new knowledge in platelet biology that will allow characterisation and prediction of key molecular and morphological changes occurring across a blood thrombus under flowing conditions as found in the blood vessels. These new tools ....A multiplex microscope platform to define molecular events in fluid systems. This project aims to develop a novel microscopy platform that will enable the visualisation and quantification of molecular events occurring under fluid shear stress. The project will generate new knowledge in platelet biology that will allow characterisation and prediction of key molecular and morphological changes occurring across a blood thrombus under flowing conditions as found in the blood vessels. These new tools and the imaging platform will have applications for researchers wishing to visualise small and rapid molecular events in four dimensions (length, width, height and across time) under fluid shear stress, which is applicable across a range of industries. The project expects to deliver the next generation of intravital microscopes that can visualise and quantify events in a challenging flow environment.Read moreRead less
5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids. Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging ....5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids. Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging flow cytometer (5D IFC) capable of continuous assessment of potentially entire blood volume in a living mice without removing fluid out of the body. The project represents a major advancement beyond any existing flow cytometer and overcome the engineering limits of state-of-art laser scanning imaging devices.Read moreRead less
Transport control in multi-species fluid suspensions. This project aims to develop novel methods of controlling multi-species particles in fluid suspensions, such as microorganisms in wounds. Physical methods of control offer additional opportunities for wound healing in the era of increased microbial resistance to antibiotics. The project will develop methods of controlling the local concentration of microorganisms, such as bacteria and cells, using wave-driven turbulent transport and active sy ....Transport control in multi-species fluid suspensions. This project aims to develop novel methods of controlling multi-species particles in fluid suspensions, such as microorganisms in wounds. Physical methods of control offer additional opportunities for wound healing in the era of increased microbial resistance to antibiotics. The project will develop methods of controlling the local concentration of microorganisms, such as bacteria and cells, using wave-driven turbulent transport and active synthetic agents. The proposed methods will also benefit applications in microfluidics, liquid metamaterials, micro-assembly and technologies for cleaning liquid surfaces. The project will advance our fundamental knowledge of particle interaction with matter waves.Read moreRead less