Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453608
Funder
Australian Research Council
Funding Amount
$579,230.00
Summary
Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimi ....Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimination improvement. The system will also be able to monitor binding of single molecules. These techniques will open new and exciting avenues for interdisciplinary research at the frontier between biological and physical sciences. The microscope will operate within an existing multi-user Optical Characterisation Facility supporting research of an established network of scientists in the Sydney area.Read moreRead less
Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basi ....Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basis of this discrepancy and to attempt to provide tools that circumvent the problem. It is expected the project will generate novel information on the environment in the plant experienced by the fungus.Read moreRead less
Improving plant productivity and human health using next generation biotechnology approaches. Both medical and plant sciences face similar technological problems in harnessing the power of modern DNA sequencing for accelerating the pace of beneficial gene function discovery. Plant and animal researchers will collaborate in this program to meet this common challenge. The research outcomes envisaged in this proposal will benefit human health by enabling more rapid discovery of genes related to obe ....Improving plant productivity and human health using next generation biotechnology approaches. Both medical and plant sciences face similar technological problems in harnessing the power of modern DNA sequencing for accelerating the pace of beneficial gene function discovery. Plant and animal researchers will collaborate in this program to meet this common challenge. The research outcomes envisaged in this proposal will benefit human health by enabling more rapid discovery of genes related to obesity, immunity, fertility, neurological function and cancer. In the plant sphere, the outcomes will shed new light on plant functions related to plant energy metabolism, vitamin biosynthesis drought tolerance and water use by crops. The research will benefit both human health and agricultural food production and quality.Read moreRead less
Characteristics of chlorophyll d-binding protein complexes: assembly of light-harvesting complexes. This project will investigate molecular mechanisms of photosynthesis in Chl d with the view to applying our findings in biotechnology and artificial photosynthesis. We will use a variety of molecular biology, proteomics and physical techniques to probe the bonding of Chl d to binding proteins. Synthetic peptide maquettes will provide a model to develop this understanding. Only two chlorophylls (a ....Characteristics of chlorophyll d-binding protein complexes: assembly of light-harvesting complexes. This project will investigate molecular mechanisms of photosynthesis in Chl d with the view to applying our findings in biotechnology and artificial photosynthesis. We will use a variety of molecular biology, proteomics and physical techniques to probe the bonding of Chl d to binding proteins. Synthetic peptide maquettes will provide a model to develop this understanding. Only two chlorophylls (a and d) have so far been found to take part in the primary reactions of photosynthesis. This research will grow our understanding of this pivotal process and underpin future developments in artificial photosynthesis and in the photonics industry.Read moreRead less
Enhanced algal biofuel production: optimising photosynthesis in Australian strains of marine algae. Algal biofuel produces a sustainable liquid fuel to help meet our future energy needs. This project will pioneer a multifaceted approach in molecular biology and photophysiology to engineer the best biofuel producers from Australian marine algae and will advance innovation in Australia's biofuel biotechnology development.
The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cance ....The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cancer agents in humans. Flavonoids, another pathway regulated in our shoot branching lines, also benefit human health. Graduate students and postdoctoral researchers will gain skills in research management, design, ethics and experimental methods pertinent to the growing biotechnology industry in Australia.Read moreRead less
Special Research Initiatives - Grant ID: SR0354787
Funder
Australian Research Council
Funding Amount
$10,000.00
Summary
Research Network for Biotechnological and Environmental Applications of Microalgae (BEAM). The network will facilitate inderdisciplinary and collaborative research into the limitations on microalgal growth leading to the development of new, commercial-scale microalgae culture systems, the production of fine chemicals, bioactive compounds and renewable fuels (hydrogen), as well as environmental applications such as monitoring the physiological state of phytoplankton in the environment, CO2 biorem ....Research Network for Biotechnological and Environmental Applications of Microalgae (BEAM). The network will facilitate inderdisciplinary and collaborative research into the limitations on microalgal growth leading to the development of new, commercial-scale microalgae culture systems, the production of fine chemicals, bioactive compounds and renewable fuels (hydrogen), as well as environmental applications such as monitoring the physiological state of phytoplankton in the environment, CO2 bioremediation and algal/bacterial systems for the bioremediation of contaminated soils. This will be achieved by applying research on photosynthetic light utilisation efficiency and carbon fixation, chlorophyll fluorescence, biochemistry of secondary metabolites, molecular biology and photobioreactor design and engineering, informed by an understanding of the ecology of these algae.Read moreRead less
Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a soluti ....Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a solution, and achieved the key technical requirements for proof of concept. This collaboration between industry, government and university partners will deliver key Australian-owned IP, for environmentally-friendly plant biofactories, and for treatment of mitochondrial genetic disorders.Read moreRead less
Biology of flowering plant male gametic cells in relation to fertilization. The world food supply is primarily dependent on plants. The continuation of an adequate food supply depends upon the success of fertilization that involves the fusion of the sperm cell with the egg cell. The entire process of fertilization can be manipulated once the specific molecules involved in its regulation have been identified. This project deals with the discovery of male gamete biomolecules involved in fertili ....Biology of flowering plant male gametic cells in relation to fertilization. The world food supply is primarily dependent on plants. The continuation of an adequate food supply depends upon the success of fertilization that involves the fusion of the sperm cell with the egg cell. The entire process of fertilization can be manipulated once the specific molecules involved in its regulation have been identified. This project deals with the discovery of male gamete biomolecules involved in fertilization. The knowledge obtained will have potential applications in developing novel breeding technologies for improved crop plants.Read moreRead less
How plants produce their biomass. This project aims to investigate mechanisms that underpin the formation of secondary walls, the bulk of biomass in plant cells. Plant cell walls are essential for plant growth and provide great raw materials for many industrial products. Understanding how cell walls are made would enable tailored plant biomass production, but understanding remains poor. The project will induce secondary walls at will and outline a framework for how secondary walls are made. The ....How plants produce their biomass. This project aims to investigate mechanisms that underpin the formation of secondary walls, the bulk of biomass in plant cells. Plant cell walls are essential for plant growth and provide great raw materials for many industrial products. Understanding how cell walls are made would enable tailored plant biomass production, but understanding remains poor. The project will induce secondary walls at will and outline a framework for how secondary walls are made. The outcomes are expected to be relevant for the fuel, feed, food and construction sectors, and thus to Australia's future.Read moreRead less