Characteristics of chlorophyll d-binding protein complexes: assembly of light-harvesting complexes. This project will investigate molecular mechanisms of photosynthesis in Chl d with the view to applying our findings in biotechnology and artificial photosynthesis. We will use a variety of molecular biology, proteomics and physical techniques to probe the bonding of Chl d to binding proteins. Synthetic peptide maquettes will provide a model to develop this understanding. Only two chlorophylls (a ....Characteristics of chlorophyll d-binding protein complexes: assembly of light-harvesting complexes. This project will investigate molecular mechanisms of photosynthesis in Chl d with the view to applying our findings in biotechnology and artificial photosynthesis. We will use a variety of molecular biology, proteomics and physical techniques to probe the bonding of Chl d to binding proteins. Synthetic peptide maquettes will provide a model to develop this understanding. Only two chlorophylls (a and d) have so far been found to take part in the primary reactions of photosynthesis. This research will grow our understanding of this pivotal process and underpin future developments in artificial photosynthesis and in the photonics industry.Read moreRead less
The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cance ....The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cancer agents in humans. Flavonoids, another pathway regulated in our shoot branching lines, also benefit human health. Graduate students and postdoctoral researchers will gain skills in research management, design, ethics and experimental methods pertinent to the growing biotechnology industry in Australia.Read moreRead less
Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a soluti ....Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a solution, and achieved the key technical requirements for proof of concept. This collaboration between industry, government and university partners will deliver key Australian-owned IP, for environmentally-friendly plant biofactories, and for treatment of mitochondrial genetic disorders.Read moreRead less
Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on ....Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on some landscapes. Rushes are also highly sensitive to small increments in nutrients in disturbed environments and thus form a management priority relating to their use in rehabilitation of degraded landscapes, such as mine sites and wetland margins. Read moreRead less
The impact of water logging on mitochondrial metabolism in leaves and roots of Grey poplar. Water logging and flooding are seasonal occurrences in a variety of environments. These events cause various damage to environments, including damage to plants. The damage to plants has traditionally been associated with annuals, such as various crop species. However larger plants, i.e. trees, are also very susceptible to water-logging and flooding. As it can take from several years to decades for tress t ....The impact of water logging on mitochondrial metabolism in leaves and roots of Grey poplar. Water logging and flooding are seasonal occurrences in a variety of environments. These events cause various damage to environments, including damage to plants. The damage to plants has traditionally been associated with annuals, such as various crop species. However larger plants, i.e. trees, are also very susceptible to water-logging and flooding. As it can take from several years to decades for tress to be established it is important to understand how trees can withstand water-logging and flooding. As tree plantation are uses for commercial and environmental purposes this knowledge will be useful to a broad spectrum of users in the community.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE120101562
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Quantifying the contribution of leaf vein networks to the leaf economics spectrum in native and agricultural species. Using a combination of eco-physiological and geometric measures this project will evaluate the influence of leaf vein networks on leaf economics. It is expected that this work will identify vein investment and network design as major sources of variability underlying species adaptive strategies, and the global leaf economics spectrum as a whole.
Biosynthetic and evolutionary pathways of red-shifted chlorophylls. This project will provide vital information for the roles of chlorophyll modification. Red-shifted chlorophylls have the potential to increase the efficiency of photosynthesis by extending the spectral limits. The switchable biosynthetic pathway of chlorophyll f will open up a new opportunity for developing new technologies.
New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less w ....New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less water.Read moreRead less
Adapting cyanobacterial bicarbonate transporters for application in crop improvement. Marine blue-green algae contribute to global primary productivity but their carbon dioxide (CO2) acquisition processes are poorly understood. The project will determine protein structure and regulatory controls present in two classes of bicarbonate transporters required for efficient photosynthesis, and use this data towards the engineering crop plants that use less water.
The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen atta ....The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, focusing on the role of terminal oxidases. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less