Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, ....Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, semi-bitter and bitter flavour, amygdalin accumulation in developing kernels, and key enzymes in the cyanogenic pathway. Almond populations segregating for these traits will be used and the data will be integrated into the Australian almond meiotic map.Read moreRead less
Investigation of almond transformation for self-fertility and virus resistance. This project addresses the long-term goal of the Australian almond industry to produce cultivars that are self-fertile and resistant to Prunus necrotic ringspot and prune dwarf viruses. Both self-sterility and virus infection can result in unreliable and often low yields. Research will address the control of gene expression relating to self-sterility and the introduction of virus resistance, via sequencing of self- ....Investigation of almond transformation for self-fertility and virus resistance. This project addresses the long-term goal of the Australian almond industry to produce cultivars that are self-fertile and resistant to Prunus necrotic ringspot and prune dwarf viruses. Both self-sterility and virus infection can result in unreliable and often low yields. Research will address the control of gene expression relating to self-sterility and the introduction of virus resistance, via sequencing of self-fertility and self-sterility genes, construct development and improved regeneration of transformed almond in vitro without the use of antibiotics.Read moreRead less
Development of cloning technology for the Australian Pig Industry. Cloning has the potential to be the most efficient of the reproductive technologies developed for increasing genetic improvement in livestock. Currently up to 5% of cloned embryos develop to term in the pig. This is higher than that reported for cattle and sheep. Moreover the use of this technology in the pig does not appear not to result in the same sorts of problems and losses seen around the time of birth in these species ....Development of cloning technology for the Australian Pig Industry. Cloning has the potential to be the most efficient of the reproductive technologies developed for increasing genetic improvement in livestock. Currently up to 5% of cloned embryos develop to term in the pig. This is higher than that reported for cattle and sheep. Moreover the use of this technology in the pig does not appear not to result in the same sorts of problems and losses seen around the time of birth in these species i.e. the majority of cloned pigs appear normal and are healthy at birth. However before cloning can be used commercially, current efficiencies need to be increased approx two fold for this to be economically viable. The aim of the present study is to improve the efficiency of our current cloning protocol and develop associated technologies such as embryo freezing to facilitate commercialisation. This will ensure that the Australian Pig Industry remains competitive at a pivotal time in its development.Read moreRead less
Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major variet ....Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major varieties. Molecular markers will be developed to accelerate the selection of improved varieties with self-fertility, high oleic acid, low linolenic acid and tolerance to Peacock spot disease.Read moreRead less
Targeted approaches to improve nitrogen use efficiency in maize. Nitrogen is an essential input required for growing high yielding quality cereal crops such as maize and wheat. Unfortunately, excessive use of nitrogen fertilizers can lead to serious environmental costs including nitrogen pollution through leaching and the significant cost in non-renewable fossil fuels used in their production. Improving nitrogen use efficiency in crops such as maize will reduce fertilizer use while ensuring lo ....Targeted approaches to improve nitrogen use efficiency in maize. Nitrogen is an essential input required for growing high yielding quality cereal crops such as maize and wheat. Unfortunately, excessive use of nitrogen fertilizers can lead to serious environmental costs including nitrogen pollution through leaching and the significant cost in non-renewable fossil fuels used in their production. Improving nitrogen use efficiency in crops such as maize will reduce fertilizer use while ensuring long-term sustainable production and harvestable yields. This collaboration with DuPont-Pioneer will focus on identifying nitrogen-linked traits in Maize that will be incorporated into new lines targeted at reducing grower dependence on nitrogen fertilizers.Read moreRead less
Cell type-specific transgene expression to increase Fe content in cereal grains. The grains industry forms a vital part of the Australian economy and farm sector. Increased iron concentrations of wheat and other cereal grains would greatly increase their nutritional value for people worldwide, thereby increasing their market value and profitability for farmers. High iron wheat would also lower the production costs of many Australian wheat products by reducing or eliminating the need for iron for ....Cell type-specific transgene expression to increase Fe content in cereal grains. The grains industry forms a vital part of the Australian economy and farm sector. Increased iron concentrations of wheat and other cereal grains would greatly increase their nutritional value for people worldwide, thereby increasing their market value and profitability for farmers. High iron wheat would also lower the production costs of many Australian wheat products by reducing or eliminating the need for iron fortification of wheat flour. High iron cereals promote healthy development of young Australians and can improve preventative healthcare by reducing the incidence of iron deficiency anaemia and biochemical deficiency.Read moreRead less
Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a nov ....Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a novel NUE technology that when transferred to plants significantly improves NUE. We will define the phenotype at the molecular, biochemical and physiological levels to maximise its adoption to other agricultural crops such as wheat, barley and maize.Read moreRead less
Developing Zn-dense, high-yielding wheat by molecular marker technology. The objective of this project is to identify pathways leading to the accumulation of zinc — an important element for human nutrition — in wheat. The project aims to provide biochemical and molecular markers for breeding programs that will facilitate the selection of superior breeding lines for improved human nutrition and seed health. This project builds on studies using a wheat diversity panel with 90 000 gene-based single ....Developing Zn-dense, high-yielding wheat by molecular marker technology. The objective of this project is to identify pathways leading to the accumulation of zinc — an important element for human nutrition — in wheat. The project aims to provide biochemical and molecular markers for breeding programs that will facilitate the selection of superior breeding lines for improved human nutrition and seed health. This project builds on studies using a wheat diversity panel with 90 000 gene-based single nucleotide polymorphism (SNP) markers, where zinc–SNP associations were identified. The project also builds on recent studies that show particular metabolites and macronutrients around anthesis are linked to improved grain zinc concentration at maturity.Read moreRead less
Control points in nitrogen uptake: enhancing the response of cereals to nitrogen supply and demand. Vast amounts of nitrogen fertiliser are applied to cereal crops to maintain yields. By uncovering what limits nitrogen uptake in cereals, this project will provide the scientific basis for improving nitrogen use efficiency and decreasing fertiliser use, with significant economic and environmental benefits.
Small molecules with large effect: The dual role of nitrogen-containing metabolites in stress tolerance and nutrient recycling. The main objective of this project is to identify drought and nutrient-deficiency responsive pathways in tolerant wheat and to provide markers to breeding programs that facilitate selection of superior breeding lines. This project builds on a pilot study conducted in rice in which tolerant-specific metabolites were identified which are representative of pathways relevan ....Small molecules with large effect: The dual role of nitrogen-containing metabolites in stress tolerance and nutrient recycling. The main objective of this project is to identify drought and nutrient-deficiency responsive pathways in tolerant wheat and to provide markers to breeding programs that facilitate selection of superior breeding lines. This project builds on a pilot study conducted in rice in which tolerant-specific metabolites were identified which are representative of pathways relevant for the protection of cells from damage through reactive oxygen species (ROS) and for nutrient (nitrogen, phosphorus, sugars) recycling under stress. Available data suggest that these pathways are also relevant in wheat. Quantitative metabolomics, genetics, and molecular tools will be used to deliver either DNA-based or metabolomics markers to breeders.Read moreRead less