Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of sy ....Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of synthetic encapsulin-Rubisco carbon-fixing nanoreactors and transform them into leaf chloroplasts to test their impact on plant photosynthesis and growth. Our genetically simpler solution will aid ongoing global efforts to deliver overdue step change improvements in agricultural productivity.Read moreRead less
How do apicomplexan parasites steal amino acids from their hosts? The single-celled parasites that cause malaria and toxoplasmosis are adept at stealing nutrients from the host animals that they infect. How they do this is, however, poorly understood. This project seeks to identify the processes by which these parasites scavenge amino acids, an essential class of nutrient, from their hosts. Using innovative experimental approaches, the project aims to identify and characterise the parasite prote ....How do apicomplexan parasites steal amino acids from their hosts? The single-celled parasites that cause malaria and toxoplasmosis are adept at stealing nutrients from the host animals that they infect. How they do this is, however, poorly understood. This project seeks to identify the processes by which these parasites scavenge amino acids, an essential class of nutrient, from their hosts. Using innovative experimental approaches, the project aims to identify and characterise the parasite proteins that mediate the uptake of different amino acids into the parasite. The intended outcomes of the project are to provide comprehensive insights into a fundamental aspect of parasite biology, and inform strategies to treat the diseases caused by these parasites by cutting off their nutrient supply.Read moreRead less
Flipping the mattress: infinite polyurethane recycling by synthetic biology. Australia is covered in billions of tonnes of plastic and yet <10% is recycled today. Polyurethane (PU) is ubiquitous in our everyday lives, from lacquer coatings to elastane clothing to durable foam padding in car seats, cushions and mattresses. Currently, there are few avenues for PU recycling and much ends up in landfill e.g., a single mattress produces 15-20kg of PU foam waste. Luckily, biodegradation of PU can occu ....Flipping the mattress: infinite polyurethane recycling by synthetic biology. Australia is covered in billions of tonnes of plastic and yet <10% is recycled today. Polyurethane (PU) is ubiquitous in our everyday lives, from lacquer coatings to elastane clothing to durable foam padding in car seats, cushions and mattresses. Currently, there are few avenues for PU recycling and much ends up in landfill e.g., a single mattress produces 15-20kg of PU foam waste. Luckily, biodegradation of PU can occur naturally via various microbial means and from insects, like Galleria mellonella larvae. The overall aim of this research project is to understand plastic biodegradation and translate nature’s solutions into flexible and efficient synthetic enzyme technologies that can sustainably recycle commonly used PU foams. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE240100068
Funder
Australian Research Council
Funding Amount
$931,950.00
Summary
Australian Advanced Metabolic Signal Discovery, and Imaging Platform. This proposal aims to establish an Australian Advanced Metabolic Signal Discovery and Imaging platform. The platform consists of an ultra-high resolution gas chromatography mass spectrometer and an imaging mass spectrometry upgrade for a second existing high resolution mass spectrometer. The facility will break barriers currently limiting discovery and localisation of metabolic changes during plant and animal development under ....Australian Advanced Metabolic Signal Discovery, and Imaging Platform. This proposal aims to establish an Australian Advanced Metabolic Signal Discovery and Imaging platform. The platform consists of an ultra-high resolution gas chromatography mass spectrometer and an imaging mass spectrometry upgrade for a second existing high resolution mass spectrometer. The facility will break barriers currently limiting discovery and localisation of metabolic changes during plant and animal development under environmental stress; integral chemical signals exchanged in host-microbe interactions; and volatile signatures linked to ecosystem health and developmental anomalies in animals. Results will inform innovative strategies to enhance biological adaptation, climate resilience and plant, animal, and ecosystem health.Read moreRead less
Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this ....Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this process. This should provide significant benefits such as a new theoretical basis to engineer crop plants for resistance against devastating diseases.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210100466
Funder
Australian Research Council
Funding Amount
$427,623.00
Summary
Decrypting chloroplast signalling in C4 photosynthesis under heat stress. This project aims to fill a critical knowledge gap in how photosynthesis, chloroplast signals, metabolism and cell specialisation are coordinated for stress acclimation in plants. It aims to dissect the complex interactions between a) cellular distress signals produced by chloroplasts with b) reactive radicals and c) plant metabolism during heat stress. It expects to provide the first insights into chloroplast signalling c ....Decrypting chloroplast signalling in C4 photosynthesis under heat stress. This project aims to fill a critical knowledge gap in how photosynthesis, chloroplast signals, metabolism and cell specialisation are coordinated for stress acclimation in plants. It aims to dissect the complex interactions between a) cellular distress signals produced by chloroplasts with b) reactive radicals and c) plant metabolism during heat stress. It expects to provide the first insights into chloroplast signalling critical for heat-tolerant C4 photosynthesis which is active in two specialised leaf cell types in cereals such as maize and sorghum. Expected outcomes include an unprecedented cell-level resolution map of chloroplast signalling, which will benefit the engineering of improved photosynthesis into crops. Read moreRead less
Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolis ....Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolism and signaling may play crucial roles in pollen growth and fruit set under heat stress. The intended outcome is the generation of critical knowledge that will advance understanding on reproductive development under heat stress, thereby providing significant benefits, such as novel ideas and solutions for improving crop yield.Read moreRead less
Unlocking secrets of fertility restoration for hybrid breeding in crops. Hybrid varieties give higher and more stable yields than conventional lines, but a cost-effective system to make hybrid seed on a commercial scale is still missing for economically important crops like wheat or barley. By elucidating the mode of action of a new type of restorer gene plus exploiting ancient or exotic wheat and barley collections this project will reveal aspects of largely understudied mechanisms underlying f ....Unlocking secrets of fertility restoration for hybrid breeding in crops. Hybrid varieties give higher and more stable yields than conventional lines, but a cost-effective system to make hybrid seed on a commercial scale is still missing for economically important crops like wheat or barley. By elucidating the mode of action of a new type of restorer gene plus exploiting ancient or exotic wheat and barley collections this project will reveal aspects of largely understudied mechanisms underlying fertility restoration in wheat and barley. The expected outcomes of the proposed research have the potential to deliver new tools for hybrid seed production programs in wheat and barley. Higher and more stable yields from hybrids will ensure food security in the face of an uncertain climate and growing human population.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210100323
Funder
Australian Research Council
Funding Amount
$431,000.00
Summary
Synthetic biology to engineer novel disease resistance in cereal crops. This project aims to engineer disease resistance in crops to dangerous fungal pathogens. The strategy is to exploit our knowledge of the plant immune system using structural biology and directed evolution of natural resistance genes, improving their ability to recognise and respond to fungal attack. Fungal pathogens cause some of the most harmful crop diseases in Australia and worldwide. The rapid evolution of fungi overcome ....Synthetic biology to engineer novel disease resistance in cereal crops. This project aims to engineer disease resistance in crops to dangerous fungal pathogens. The strategy is to exploit our knowledge of the plant immune system using structural biology and directed evolution of natural resistance genes, improving their ability to recognise and respond to fungal attack. Fungal pathogens cause some of the most harmful crop diseases in Australia and worldwide. The rapid evolution of fungi overcomes natural plant resistance and management of these diseases is a major challenge to agriculture. Expected outcomes of the project include engineered wheat plants with more effective disease resistance, reducing fungicide usage. This project intends to accelerate crop breeding and contribute to world food security.Read moreRead less