Using the fractionation of hydrogen and carbon isotopes to analyse the mechanisms of the primary processes of photosynthesis. The primary processes of CO2 fixation and reduction in photosynthesis leave their signatures in the isotopic composition of organic matter. Although these signatures are used widely in geochemistry, biology and climatology to infer the dynamics and history of the biosphere, the information they provide about the mechanisms of the processes that produce them has not been e ....Using the fractionation of hydrogen and carbon isotopes to analyse the mechanisms of the primary processes of photosynthesis. The primary processes of CO2 fixation and reduction in photosynthesis leave their signatures in the isotopic composition of organic matter. Although these signatures are used widely in geochemistry, biology and climatology to infer the dynamics and history of the biosphere, the information they provide about the mechanisms of the processes that produce them has not been exploited fully. We propose to map the underlying biochemistry responsible for fractionation of hydrogen isotopes, to assess its ability to indicate the water relations of plants, and to use carbon-isotope discrimination to probe the catalytic chemistry of the CO2-fixing enzyme, Rubisco.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0214135
Funder
Australian Research Council
Funding Amount
$492,000.00
Summary
High performance protein crystallography. This proposal will provide state of the art high performance facilities for protein crystallography, bringing together the major structural biology groups in NSW and the ACT. A renewed focus on protein crystal structures will stimulate new interpretation and utilization of the vast amount of data that has come from genomics, especially the sequencing of the human genome. The proposed facility will generate new research collaborations between the partn ....High performance protein crystallography. This proposal will provide state of the art high performance facilities for protein crystallography, bringing together the major structural biology groups in NSW and the ACT. A renewed focus on protein crystal structures will stimulate new interpretation and utilization of the vast amount of data that has come from genomics, especially the sequencing of the human genome. The proposed facility will generate new research collaborations between the partner institutions which will result in advances in basic life sciences, biotechnology and biopharmaceuticals. The facility will complement regional initiatives in functional genomics, bioinformatics, proteomics and high-field NMR spectroscopy.Read moreRead less
Structures and Functions of Bacterial Replisomal Proteins. DNA replication in all organisms requires many proteins to interact in a structure called the replisome. The bacterial replisome is assembled about the DnaB helicase, a motor protein that moves along DNA, separating the strands of duplex regions in its path. This project aims to develop understanding of the chemistry of DnaB and other replisomal proteins: their structures, how they work, and how they interact to assemble the replisome. T ....Structures and Functions of Bacterial Replisomal Proteins. DNA replication in all organisms requires many proteins to interact in a structure called the replisome. The bacterial replisome is assembled about the DnaB helicase, a motor protein that moves along DNA, separating the strands of duplex regions in its path. This project aims to develop understanding of the chemistry of DnaB and other replisomal proteins: their structures, how they work, and how they interact to assemble the replisome. This has the potential to lead to design of new antibacterial drugs.
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Exploring the catalytic role of the Rubisco small subunit: a new target for improving carbon dioxide-fixation in plants. This project uses new biotechnological tools to improve the performance of the photosynthetic protein Rubisco, the primary carbon dioxide-fixing enzyme in plants. By supercharging photosynthesis, this research will help to boost yield and reduce water and nitrogen use in crops.
Rubisco for all climates: unlocking the enzyme's structure-function relations for more efficient photosynthesis. This projects biotechnological research will identify structural features in the carbon dioxide (CO2)-capturing enzyme from plants that improve its performance, particularly at warmer temperatures. This knowledge is vital for predicting the influence of climate change on crop productivity and paving the way for supercharging photosynthesis to boost crop performance.
The metabolic and enzymatic regulation of C4 photosynthesis and its impact on photosynthetic productivity. Australia's tropical pastures are dominated by plants utilising the C4 photosynthetic pathway. World wide C4 grasslands contribute to approximately 20% of global primary productivity. C4 plants also include important crop species such as maize, sorghum and sugar cane and are considered ideal species for bio-fuel production. This project will use a novel functional genomic/metabolomics appro ....The metabolic and enzymatic regulation of C4 photosynthesis and its impact on photosynthetic productivity. Australia's tropical pastures are dominated by plants utilising the C4 photosynthetic pathway. World wide C4 grasslands contribute to approximately 20% of global primary productivity. C4 plants also include important crop species such as maize, sorghum and sugar cane and are considered ideal species for bio-fuel production. This project will use a novel functional genomic/metabolomics approach to provide fundamental insights into the biochemical regulation of C4 photosynthesis under different environmental conditions. This will aid in the development of mathematical models of C4 photosynthesis required in climate models of CO2 exchange and enhance our ability to improve photosynthetic performance of agricultural species.Read moreRead less