The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including diseas ....The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including disease susceptibility, and the same seems to apply in plants. This project aims to apply the genome sequences for Brassica species to detect CNVs from re-sequencing data. Knowing how this variation affects an individual or line’s disease susceptibility, especially to the devastating fungal pathogen blackleg, could improve plant protection strategies and crop production.Read moreRead less
Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum. Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and d ....Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum. Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and develop transcriptional and biochemical markers for metabolic resistance diagnosis. Herbicide-metabolising gene discovery, characterisation and marker development will greatly extend the currently limited knowledge and understanding of metabolic resistance and help achieve sustainable weed management.Read moreRead less
Nanoassembling agrochemicals - a new paradigm in delivery for enviro-friendly crop treatment. This project will determine innovative new forms of preparing agrochemicals that will deliver significant environmental and economical benefits to Australia and the Australian-based agrochemical industry. Reduced chemical and water usage will contribute to an environmentally sustainable Australia, while more economical farming will benefit rural Australia.
Sterol interference as a new approach to the control of insect pests of crops. This project aims to develop a new approach to control chewing insect pests of crops. This will be achieved by interfering with insect sterol metabolism so that they fail to grow and reproduce normally.
Towards improving the yield of Canola and other Brassicas during drought. One of the major problems faced by world agriculture is drought; this project should improve the yield of Canola during moderate to severe droughts. Significantly, this project includes both "traditional" non-genetically modified (GM) strategies and GM strategies to maximise the market for our drought-tolerant canola both in Australia and overseas.
Mapping recombination blocks in Brassica. DNA technology provides new ways to study genomes. Understanding how the genome behaves during plant breeding will help design strategies for the breeding and selection of improved crop plants.
Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowin ....Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowing very high level glyphosate resistance, and to elucidate the evolutionary trajectory of glyphosate resistance in E. indica. This will advance our current knowledge and understanding of resistance evolution and have impact on resistance management.Read moreRead less
Characterising structural variation in the canola genome. Characterising structural variation in the canola genome. This project aims to develop and apply genomic tools to identify and characterise structural genome variation in canola, a major Australian export crop, to better understand genome evolution and accelerate canola breeding. Advances in DNA sequencing revolutionise our understanding of crop genomes, their evolution and impact on the inheritance on agronomic traits. Variation of genom ....Characterising structural variation in the canola genome. Characterising structural variation in the canola genome. This project aims to develop and apply genomic tools to identify and characterise structural genome variation in canola, a major Australian export crop, to better understand genome evolution and accelerate canola breeding. Advances in DNA sequencing revolutionise our understanding of crop genomes, their evolution and impact on the inheritance on agronomic traits. Variation of genome structure between individuals could be important in the inheritance of important agronomic traits. Recent advances in technology permit the detailed characterisation of structural variation on a previously unfeasible scale. Anticipated outcomes are enhanced global food security, supporting rural Australian economies, and accelerating the improvement of other major crops.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210100398
Funder
Australian Research Council
Funding Amount
$448,365.00
Summary
The Life And Death Of Plant Genes. My recent work has demonstrated that in contrast to animal genes, many plant genes show presence/absence variation within a species, with associated trait variation. In this project, I will explore models of gene birth and death by comparing genomes of Brassicaceae, including the model Arabidopsis and Brassica crop species. By comparing many genomes I will learn how new genes were born. I will build models that predict the likelihood of gene loss based on a gen ....The Life And Death Of Plant Genes. My recent work has demonstrated that in contrast to animal genes, many plant genes show presence/absence variation within a species, with associated trait variation. In this project, I will explore models of gene birth and death by comparing genomes of Brassicaceae, including the model Arabidopsis and Brassica crop species. By comparing many genomes I will learn how new genes were born. I will build models that predict the likelihood of gene loss based on a gene’s physical environment, function, and expression. The project will build on our understanding of plant genetic diversity. Expected outcomes of this research include the identification of key genomic elements in gene birth and loss and support strategies to improve plant cultivars.Read moreRead less
Defining the Brassica pan-genome and establishing methods for gene conversion based crop improvement. Gene content varies between individual varieties. The project aims to apply novel genomic tools to identify and characterise the fixed and variable gene content in the important crop canola and use this to understand genome evolution as well as develop tools to accelerate canola breeding. The project team have developed and used a high-resolution genotyping approach to demonstrate that gene conv ....Defining the Brassica pan-genome and establishing methods for gene conversion based crop improvement. Gene content varies between individual varieties. The project aims to apply novel genomic tools to identify and characterise the fixed and variable gene content in the important crop canola and use this to understand genome evolution as well as develop tools to accelerate canola breeding. The project team have developed and used a high-resolution genotyping approach to demonstrate that gene conversions, short recombination events which lead to the non-reciprocal exchange of genomic regions during meiosis, are abundant in crop genomes. The project aims to develop methods and resources to characterise gene conversion in canola and establish a basis for gene conversion based crop improvement.Read moreRead less