Discovery Early Career Researcher Award - Grant ID: DE120100794
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Revealing dynamic mechanisms controlling pluripotency in mammalian stem cells and embryos. Every cell of our mature bodies originates from 'pluripotent' cells present in the early mammalian embryo. These cells can be captured and grown in plastic dishes. The project will use imaging methods to reveal how gene regulatory molecules control pluripotent cells in the embryo and in culture.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE130100090
Funder
Australian Research Council
Funding Amount
$700,000.00
Summary
Three-dimensional cryo electron microscopy facility. The three-dimensional cryo-electron microscopy facility will let us visualise plants, pathogens and nanomachines with resolution not previously possible allowing us to see into cells and diseases with vastly more detail. Our world-class experts will provide regional and national researchers access to cutting-edge technology complementary to the Australian Synchrotron.
Imaging transcription factors in living mammalian embryos to reveal cell-to-cell variability. The mechanisms controlling how single cells activate different genes are typically studied in cells grown in culture dishes. This project will apply novel imaging methods to study how gene regulatory molecules control cells in living mouse embryos.
Understanding how Plasmepsin V directs export of malaria virulence proteins to the host cell. This project aims to characterise how malaria parasites survive and manipulate infected host cells by exporting virulence proteins. This project may identify essential proteins that allow the malaria parasite to transform the host in order to survive, replicate and hide from the immune system and provide new data on protein export in liver-stages.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100165
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
Electron microscopy cryopreparation facility for biomedical research. The proposed cryopreparation facility will allow cell and molecular biologists and material scientists in the region to prepare samples for ultrastructural research not currently possible due to insufficient local resources, and will thus significantly boost their research. The facility will support a wide range of world class medical and material scientists, including those visiting the Australian Synchrotron, whose research ....Electron microscopy cryopreparation facility for biomedical research. The proposed cryopreparation facility will allow cell and molecular biologists and material scientists in the region to prepare samples for ultrastructural research not currently possible due to insufficient local resources, and will thus significantly boost their research. The facility will support a wide range of world class medical and material scientists, including those visiting the Australian Synchrotron, whose research in health sciences and advanced materials characterisation facilitates the goals of promoting and maintaining good health and frontier technologies. The instrumentation will enhance training capacity in the region and provide young Australian scientists with direct experience of modern electron microscopy techniques.Read moreRead less
Understanding how cells regulate self eating during starvation and stress. This project aims to investigate how autophagosomes are built during autophagy by using advanced multi-modal imaging and unique gene-edited human cell lines. This project expects to generate new knowledge on how a family of evolutionary conserved proteins regulate autophagosome formation during starvation and stress conditions. Expected outcomes include the development of frontier imaging technologies that can be subseque ....Understanding how cells regulate self eating during starvation and stress. This project aims to investigate how autophagosomes are built during autophagy by using advanced multi-modal imaging and unique gene-edited human cell lines. This project expects to generate new knowledge on how a family of evolutionary conserved proteins regulate autophagosome formation during starvation and stress conditions. Expected outcomes include the development of frontier imaging technologies that can be subsequently utilised for the advancement of any field of cell biology. This should provide significant benefits by placing Australia at the forefront of cell biology technologies and increasing our understanding of how plant and human cells can protect themselves during starvation and stress.
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A tale of two genomes: integrating mitochondrial biogenesis into the cell cycle and metabolic control. The human genome is cordoned into two distinct compartments in our cells. Most genes are in the nucleus, while a distinct set of genes are held within our mitochondria. Using yeast as a model organism, this project will provide a holistic view of how expression of the two genomes is coordinated.
Characterisation of membrane protein ubiquitination by MARCH ligases. The goal of the project is to understand how a family of enzymes called MARCHs regulate expression and localisation of immunoregulatory receptors within cells by post-translational addition of a small protein tag called Ubiquitin. The aims are to decipher the ubiquitination patterns produced by the MARCHs; identify the E2 ligases used by the MARCHs to produce distinct Ub codes; and apply a new proteomic pipeline to identify no ....Characterisation of membrane protein ubiquitination by MARCH ligases. The goal of the project is to understand how a family of enzymes called MARCHs regulate expression and localisation of immunoregulatory receptors within cells by post-translational addition of a small protein tag called Ubiquitin. The aims are to decipher the ubiquitination patterns produced by the MARCHs; identify the E2 ligases used by the MARCHs to produce distinct Ub codes; and apply a new proteomic pipeline to identify novel representative MARCH substrates in mice deficient in six different MARCHs. It is anticipated the project will reveal novel insights into a fundamental cell biological process of major significance for regulation of protein expression and trafficking in cells of the immune system.Read moreRead less
Membrane trafficking and endosome to trans-Golgi network retrograde pathways. This project will study newly discovered and essential transport highways in cells, which connect the secretory and internalisation pathways. This research will enhance understandings of how molecules are transported along specific highways in cells. By training students, the project will contribute to the expertise of cell biology in Australia.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100202
Funder
Australian Research Council
Funding Amount
$255,120.00
Summary
Three-dimensional cryo correlative light and electron microscopy facility. This project aims to establish a three-dimensional (3D) cryo-correlative light and electron microscopy facility. The facility will integrate light microscopy with high resolution cryo-electron tomography and 3D slice-and-view focused ion beam scanning electron microscopy. The open access facility should create new capabilities for Australian researchers to tag biological events and structures with fluorescence markers and ....Three-dimensional cryo correlative light and electron microscopy facility. This project aims to establish a three-dimensional (3D) cryo-correlative light and electron microscopy facility. The facility will integrate light microscopy with high resolution cryo-electron tomography and 3D slice-and-view focused ion beam scanning electron microscopy. The open access facility should create new capabilities for Australian researchers to tag biological events and structures with fluorescence markers and image them using the currently highest resolution 3D imaging techniques for biological matter. The facility expects to reveal fundamental insights into cell and structural biology, and help drive innovation in agriculture, pharmaceutics, and biomaterials.Read moreRead less