Single vesicle dynamics and the control of secretion. This project investigates secretion and tests a new model for secretory control. Its outcomes will further our knowledge in this important area and may be significant in the longer term for the treatment of secretory diseases.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100037
Funder
Australian Research Council
Funding Amount
$170,000.00
Summary
Laser microdissection facility. Laser microdissection facility: Laser microdissection (LMD) is a proven and effective approach to isolate pure cell populations from heterogeneous tissue samples in order to analyse DNA, RNA, and protein content. LMD provides an important advantage to research engaged in basic biological research as it allows for molecular profiling at the cellular level that can not be achieved from whole tissue samples. This project will provide a LMD facility aimed at understan ....Laser microdissection facility. Laser microdissection facility: Laser microdissection (LMD) is a proven and effective approach to isolate pure cell populations from heterogeneous tissue samples in order to analyse DNA, RNA, and protein content. LMD provides an important advantage to research engaged in basic biological research as it allows for molecular profiling at the cellular level that can not be achieved from whole tissue samples. This project will provide a LMD facility aimed at understanding many important biologically cellular mechanisms.Read moreRead less
Amino acids as nutrients - the molecular basis of amino acid absorption in kidney and intestine. Nutrition is a critical factor for well being and health. This is highlighted by the increase in frequency of type II diabetes and the obesity problem that all societies with a western diet face. Most studies in nutrition focus on fat and carbohydrates because of their direct involvement in obesity and diabetes. Proteins and the resulting peptides and amino acids, however, form 10-15% of our nutritio ....Amino acids as nutrients - the molecular basis of amino acid absorption in kidney and intestine. Nutrition is a critical factor for well being and health. This is highlighted by the increase in frequency of type II diabetes and the obesity problem that all societies with a western diet face. Most studies in nutrition focus on fat and carbohydrates because of their direct involvement in obesity and diabetes. Proteins and the resulting peptides and amino acids, however, form 10-15% of our nutrition. At a time where protein-rich diets are recommended to fight obesity and diabetes, it is important to understand the basis of protein absorption and metabolism. This project investigates the mechanism and mediators of amino acid absorption in kidney and intestine. Read moreRead less
A new model for secreton in epithelial cells. This proposal sets out to test a new model for secretion that we have developed in the light of recent experimental data. The project outcomes will advance our understanding of normal processes of secretion and may be important in understanding disease. We will develop cutting-edge techniques of microscopy which will place Australia at the forefront of this exciting field. The project will bring benefit to the Australian scientific community through ....A new model for secreton in epithelial cells. This proposal sets out to test a new model for secretion that we have developed in the light of recent experimental data. The project outcomes will advance our understanding of normal processes of secretion and may be important in understanding disease. We will develop cutting-edge techniques of microscopy which will place Australia at the forefront of this exciting field. The project will bring benefit to the Australian scientific community through interactions and collaborations with other scientists in Australia and internationally and will benefit early-career scientists, training them in novel methods and allowing them to develop their research expertise and profile and enabling them to compete on the world science stage. Read moreRead less
Sugar and water handling by honeyeaters and sunbirds. Honeyeaters are the major vertebrate pollinators in Australia, playing a vital role in maintenance of healthy ecosystems. These birds are faced with extreme physiological challenges due to their diet, ingesting 2-5 times their body mass in nectar to obtain their daily sugar requirement. Nectarivores are, consequently, masters at processing and assimilating sugars and water; comparative physiology of these charismatic birds will provide deep u ....Sugar and water handling by honeyeaters and sunbirds. Honeyeaters are the major vertebrate pollinators in Australia, playing a vital role in maintenance of healthy ecosystems. These birds are faced with extreme physiological challenges due to their diet, ingesting 2-5 times their body mass in nectar to obtain their daily sugar requirement. Nectarivores are, consequently, masters at processing and assimilating sugars and water; comparative physiology of these charismatic birds will provide deep understanding of sugar and water handling mechanisms. Understanding gut and renal physiology and morphology contributes to the broad base of knowledge required to address pathological and clinical conditions in man, and will enhance our ability to predict effects of environmental change on these birds.Read moreRead less
The sulfate anion transporter gene, Sat1: physiology, regulation and developmental expression. Sulfate is an essential nutrient for cell growth and survival. The kidneys and liver help regulate sulfate levels in the body, by yet unknown mechanisms. Recently, we cloned a gene, Sat1, expressed in mouse liver and kidneys, which may be responsible for body sulfate maintenance. In this study, we will determine the physiological importance of Sat1 in cell growth/survival and in controlling body sulfa ....The sulfate anion transporter gene, Sat1: physiology, regulation and developmental expression. Sulfate is an essential nutrient for cell growth and survival. The kidneys and liver help regulate sulfate levels in the body, by yet unknown mechanisms. Recently, we cloned a gene, Sat1, expressed in mouse liver and kidneys, which may be responsible for body sulfate maintenance. In this study, we will determine the physiological importance of Sat1 in cell growth/survival and in controlling body sulfate levels. We will generate and characterise a Sat1 lacking mouse, study its expression during development and its effects on other genes. We will elucidate how body sulfate levels are maintained and its importance in cell growth/development.Read moreRead less
Unified framework of intestinal motility. The project aims to establish how a few fundamental mechanisms determine the large repertoire of intestinal motor patterns responsible for moving nutrients along the digestive tract. The project will combine experimental and theoretical data, with biomechanical and electrophysiological models to create a new understanding of this essential function of the body.