Regulation of nuclear localisation of the AreA transcription factor in Aspergillus nidulans. An understanding of the means by which the expression of genes is regulated is of fundamental significance. Changes in gene expression are central to the development, growth and viability of all cells and their response to environmental changes/stresses. This study uses the fungus Aspergillus nidulans as an excellent molecular genetic tool to investigate how a key regulatory protein controls gene expres ....Regulation of nuclear localisation of the AreA transcription factor in Aspergillus nidulans. An understanding of the means by which the expression of genes is regulated is of fundamental significance. Changes in gene expression are central to the development, growth and viability of all cells and their response to environmental changes/stresses. This study uses the fungus Aspergillus nidulans as an excellent molecular genetic tool to investigate how a key regulatory protein controls gene expression in response to nitrogen starvation stress. Our understanding of these dynamic processes informs our approaches to the development of cancer therapies, to commercial biotechnology application and to control of human, plant and animal pathogens in which the infectious process is triggered by environmental stress. Read moreRead less
Mechanistic characterisation of genotype x environment interactions in sorghum and arabidopsis. Sorghum is an economically important cereal crop for Australia. In Australia, sorghum is used as a staple animal feed and it is very important for the live stock industry. With the predicted changes of temperature and rainfall patterns due to climate change, negative effects on sorghum yield are expected, which can have adverse effects on Australian economy. Our studies will identify and mark genes th ....Mechanistic characterisation of genotype x environment interactions in sorghum and arabidopsis. Sorghum is an economically important cereal crop for Australia. In Australia, sorghum is used as a staple animal feed and it is very important for the live stock industry. With the predicted changes of temperature and rainfall patterns due to climate change, negative effects on sorghum yield are expected, which can have adverse effects on Australian economy. Our studies will identify and mark genes that regulate flowering and seed production in sorghum in response to changes in temperature and light interactions. These studies will help to develop novel sorghum varieties with desirable characters through plant-breeding programmes.Read moreRead less
Molecular genetic analyses of trinucleotide repeat expansions. Several neuronal diseases like Huntington's disease, Frederick's ataxia and fragile X syndrome are caused by expansion of trinucleotide repeat sequences in the deoxyribonucleic acid (DNA). These diseases show progressive severity in subsequent generations. Here we use a simple plant model with a very similar DNA mutation to study the genetic basis of repeat expansions over several generations across populations. This proposal will im ....Molecular genetic analyses of trinucleotide repeat expansions. Several neuronal diseases like Huntington's disease, Frederick's ataxia and fragile X syndrome are caused by expansion of trinucleotide repeat sequences in the deoxyribonucleic acid (DNA). These diseases show progressive severity in subsequent generations. Here we use a simple plant model with a very similar DNA mutation to study the genetic basis of repeat expansions over several generations across populations. This proposal will improve our mechanistic understanding of genetic diseases in populations. In addition, this proposal is expected to lead to identification of potential targets and technologies that would be of interest to Australian industry.Read moreRead less
Unravelling the biochemical fingerprint of Australian native plants for sustainable farm forestry and other applications. Dryland salinity is an issue of national significance due to its impact on primary industries which contribute billions of dollars to our economy. However, millions of hectares of arable land are now affected by salinity, with devastating effects on crops, native plants, water quality and wildlife. This project works with the rural community and exploits the unique gene poo ....Unravelling the biochemical fingerprint of Australian native plants for sustainable farm forestry and other applications. Dryland salinity is an issue of national significance due to its impact on primary industries which contribute billions of dollars to our economy. However, millions of hectares of arable land are now affected by salinity, with devastating effects on crops, native plants, water quality and wildlife. This project works with the rural community and exploits the unique gene pool of certain Australian salinity-tolerant plants for environmental benefits (revegetation, salinity control) and simultaneous economic returns through using these for timber and perennial fodder. The project thus addresses the national priorities of preventing the expansion of salinity, putting it to sustainable uses and preserving biodiversity.Read moreRead less
Understanding how cells compact and segregate DNA in vertebrates. How a cell compacts and divides its DNA is still a major unanswered question in biology. This project will determine the way in which a cell compacts its DNA nearly ten thousand fold to allow the faithful and accurate segregation to daughter nuclei.
Mobility of log-dependent invertebrates in fragmented forest. Clearing of Australian native forests continues apace. This leads to declines in total forest area and creates isolated remnants with depleted woody debris. Critical forest processes driven by invertebrates occur in this debris. Despite its major economic and conservation significance, there is little information on the impacts of habitat loss and alteration on organisms in decomposing logs. We will utilize recent advances in popu ....Mobility of log-dependent invertebrates in fragmented forest. Clearing of Australian native forests continues apace. This leads to declines in total forest area and creates isolated remnants with depleted woody debris. Critical forest processes driven by invertebrates occur in this debris. Despite its major economic and conservation significance, there is little information on the impacts of habitat loss and alteration on organisms in decomposing logs. We will utilize recent advances in population genetics to compare impacts on a suite of log-dependent invertebrates in an islands-of-bush-in-a-sea-of-pines model. This will facilitate improvements in conservation and sustainable management outcomes in forests.Read moreRead less
Improved biomonitoring of urban freshwater ecosystems using DNA barcodes. This project aims to provide rapid diagnostic detection of environmental change in aquatic ecosystems at reduced cost. Next-generation sequencing (NGS) can transform monitoring of aquatic ecosystems by identifying many species in multiple biological samples. This project aims to use NGS technology to identify macroinvertebrate species as biological indicators in urban freshwater ecosystems to provide more rapid, sensitive ....Improved biomonitoring of urban freshwater ecosystems using DNA barcodes. This project aims to provide rapid diagnostic detection of environmental change in aquatic ecosystems at reduced cost. Next-generation sequencing (NGS) can transform monitoring of aquatic ecosystems by identifying many species in multiple biological samples. This project aims to use NGS technology to identify macroinvertebrate species as biological indicators in urban freshwater ecosystems to provide more rapid, sensitive and cost-effective monitoring compared to traditional methods. The project uses DNA barcodes that identify species to detect species in samples processed with NGS. The project also plans to use previously collected samples to explore species diversity and detect indicator species.Read moreRead less
Fisheries genomics of snapper in Australia and New Zealand Waters. This industry-driven project aims to assemble a strategic research alliance to generate and apply knowledge to a highly significant fisheries resource. It involves collaboration between the five major state government fisheries agencies in Australia, the New Zealand’s Crown Research Institute for seafood and two Australian labs with leadership in fish genetics and genomics. It expects to generate and integrate genomic, environmen ....Fisheries genomics of snapper in Australia and New Zealand Waters. This industry-driven project aims to assemble a strategic research alliance to generate and apply knowledge to a highly significant fisheries resource. It involves collaboration between the five major state government fisheries agencies in Australia, the New Zealand’s Crown Research Institute for seafood and two Australian labs with leadership in fish genetics and genomics. It expects to generate and integrate genomic, environmental and phenotypic datasets for snapper populations from across vast coastal regions of the two countries. The outcomes should substantially enhance intra- and inter-jurisdictional fisheries management and aquaculture initiatives, providing commercial, social and environmental benefits for many stakeholders.Read moreRead less
Genomics for persistence of Australian freshwater fish. Biodiversity faces an unpredictable cocktail of impacts and global environmental change, against which the best insurance is genetic diversity. We will develop genomic measures of ecological-genetic functions and evolutionary potential for managing Australian freshwater fish.
The success and rapid evolution of invasive species: resolving a genetic paradox. Invasive species have an enormous economic impact on trade, agriculture, aquaculture, fisheries and eco-tourism in Australia. In order to negate such detrimental impacts, integrated strategies need to be formulated to effectively control and eradicate introduced species. Our limited knowledge of the factors underlying the success of invasive species has hampered such efforts. A detailed understanding of the invasio ....The success and rapid evolution of invasive species: resolving a genetic paradox. Invasive species have an enormous economic impact on trade, agriculture, aquaculture, fisheries and eco-tourism in Australia. In order to negate such detrimental impacts, integrated strategies need to be formulated to effectively control and eradicate introduced species. Our limited knowledge of the factors underlying the success of invasive species has hampered such efforts. A detailed understanding of the invasion dynamics of introduced species is essential for identifying potential weaknesses that can be exploited in management strategies. My research will examine the invasion dynamics of a successful invasive species, the delicate skink, providing information directly relevant to the management of introduced species in Australia.Read moreRead less