Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100127
Funder
Australian Research Council
Funding Amount
$355,000.00
Summary
Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein funct ....Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein function inside living cells. The new equipment is designed to provide a fast super-resolution imaging system to study the intracellular dynamics of proteins in vitro and a super-resolution microscope to visualise structures and assemblies inside microbes with a resolution of tens of nanometres, putting in vitro biochemistry into the context of a living cell. Read moreRead less
Optimisation of functional mesoporous materials for low-abundance biomarkers quantification towards biodiagnostic applications. Using state-of-the-art nanotechnology, a novel approach will be developed to quantitatively analyse low abundance peptides and proteins. This will aid in the detection of a biomarker in osteoarthritis, a disease affecting 3.85 million Australians. This project has significance for the early diagnosis and improved treatment of this disease.
A novel magnetic resonance imaging (MRI) technique to characterise white matter microstructure in the brain. Integrity of the cellular architecture of brain white matter (WM) is vital to normal signal conduction and is disrupted in diseases such as multiple sclerosis. Due to their characteristic molecular arrangements, WM microstructures have distinct magnetic susceptibility characteristics that can be detected with high-field and ultra high-field magnetic resonance imaging (MRI). The objective ....A novel magnetic resonance imaging (MRI) technique to characterise white matter microstructure in the brain. Integrity of the cellular architecture of brain white matter (WM) is vital to normal signal conduction and is disrupted in diseases such as multiple sclerosis. Due to their characteristic molecular arrangements, WM microstructures have distinct magnetic susceptibility characteristics that can be detected with high-field and ultra high-field magnetic resonance imaging (MRI). The objective of this project is to develop and validate a novel method of mapping susceptibility effects at high (sub-voxel) resolution with MRI. The outcomes will be a more comprehensive understanding of the relationship between changes in MRI signal and WM microarchitecture and improved susceptibility mapping that may lead to earlier diagnosis and more effective therapeutic monitoring.Read moreRead less
New Directions in Bayesian Statistics: formulation, computation and application to exemplar challenges. Bayesian statistics is a fundamental statistical and machine learning approach for density estimation, data analysis and inference. However, there remain open questions regarding the formulation of the model, the likelihood and priors, and efficient computation. This project proposes new approaches that address these issues, and applies them to two exemplar challenges: the impact of climate ch ....New Directions in Bayesian Statistics: formulation, computation and application to exemplar challenges. Bayesian statistics is a fundamental statistical and machine learning approach for density estimation, data analysis and inference. However, there remain open questions regarding the formulation of the model, the likelihood and priors, and efficient computation. This project proposes new approaches that address these issues, and applies them to two exemplar challenges: the impact of climate change on the Great Barrier Reef and better understanding neurological diseases related aging, in particular Parkinson's Disease. Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE190100641
Funder
Australian Research Council
Funding Amount
$422,079.00
Summary
Brillouin microscopy for high-speed imaging of rigidity within cells. This project aims to improve the sensitivity and speed of Brillouin microscopes. Brillouin microscopes use light to measure the stiffness of samples in 3D without requiring physical access, allowing their use in inaccessible locations such as the interior of cells or within intact tissue. However, Brillouin microscopes are too slow to be used in most research. This project introduces a new approach based on different optical p ....Brillouin microscopy for high-speed imaging of rigidity within cells. This project aims to improve the sensitivity and speed of Brillouin microscopes. Brillouin microscopes use light to measure the stiffness of samples in 3D without requiring physical access, allowing their use in inaccessible locations such as the interior of cells or within intact tissue. However, Brillouin microscopes are too slow to be used in most research. This project introduces a new approach based on different optical physics that is expected to enable faster and more precise imaging. The microscope will be used to study the movement of amoeba, where it is expected to reveal the controlled stiffening and fluidising of the different regions of protoplasm believed to underlie the cell mobility.Read moreRead less
Probe-free biophysical force and torque measurements with optical tweezers. This project aims to develop probe-free biophysical force and torque measurement methods based on optical tweezers. Many areas of research in cell biology are hampered by a lack of quantitative force measurements. This project aims to provide accurate quantitative measurements to enable in-depth understanding of forces at work during cell division, properties of blood cells and sperm motility which could generate further ....Probe-free biophysical force and torque measurements with optical tweezers. This project aims to develop probe-free biophysical force and torque measurement methods based on optical tweezers. Many areas of research in cell biology are hampered by a lack of quantitative force measurements. This project aims to provide accurate quantitative measurements to enable in-depth understanding of forces at work during cell division, properties of blood cells and sperm motility which could generate further research leading to health benefits.Read moreRead less
Early Palaeozoic radiolarian evolution. This project will apply a new transformative technology, X-ray micro computed tomography, to the study of Early Palaeozoic (530-300 million year old) radiolarian microfossils. It is expected that this will allow, for the first time, non-destructive examination to elucidate the internal skeletal architecture of these fossils that is critical to understanding their evolution. Computer reconstruction of three-dimensional images will reveal details upon which ....Early Palaeozoic radiolarian evolution. This project will apply a new transformative technology, X-ray micro computed tomography, to the study of Early Palaeozoic (530-300 million year old) radiolarian microfossils. It is expected that this will allow, for the first time, non-destructive examination to elucidate the internal skeletal architecture of these fossils that is critical to understanding their evolution. Computer reconstruction of three-dimensional images will reveal details upon which an understanding of early phylogenetic relationships within this phylum can be developed. This in turn will allow realisation of the full biostratigraphic potential of this important long-ranging group of marine protozoans that commonly occur in great abundance in deep marine sedimentary rocks.Read moreRead less
The reptile-mammal jaw transition as revealed by 3D imaging, embryology, and computational biomechanics: new approaches to some age old questions. The extraordinary transformation of the mammalian middle ear through biomechanical 're-tooling' of the ancestral reptilian jaw joint, also recapitulated in mammalian development, yielded the greatly improved feeding and hearing efficiency that are key to the mammalian success story. Building on recent methodological advances, this project will employ ....The reptile-mammal jaw transition as revealed by 3D imaging, embryology, and computational biomechanics: new approaches to some age old questions. The extraordinary transformation of the mammalian middle ear through biomechanical 're-tooling' of the ancestral reptilian jaw joint, also recapitulated in mammalian development, yielded the greatly improved feeding and hearing efficiency that are key to the mammalian success story. Building on recent methodological advances, this project will employ cutting-edge two-dimensional protocols to explain these mechanical details of this transition. This includes integration of the first undistorted embryological soft-tissue evidence for muscular arrangements in the transitioning jaw. Through vastly improving on conventional two-dimensional approaches, the project will maintain Australia's leading position in the fast developing field of virtual biomechanics.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100121
Funder
Australian Research Council
Funding Amount
$270,000.00
Summary
An integrated system for characterisation of mechanical behaviour of bio- and nanomaterials at micro and nano scales in Queensland. Australia's material sciences will benefit from a new integrated system capable of microforce and nanomechanical testing of biomaterials, polymers and thin films, medical devices and electronics at the micro and nano scales. This facility will support ground-breaking research. It will help promote strategic collaboration and ensure the competitiveness of related and ....An integrated system for characterisation of mechanical behaviour of bio- and nanomaterials at micro and nano scales in Queensland. Australia's material sciences will benefit from a new integrated system capable of microforce and nanomechanical testing of biomaterials, polymers and thin films, medical devices and electronics at the micro and nano scales. This facility will support ground-breaking research. It will help promote strategic collaboration and ensure the competitiveness of related and emerging industries.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150101842
Funder
Australian Research Council
Funding Amount
$345,000.00
Summary
New mathematical models for capturing heterogeneity of human brain tissue. This project aims to understand the impact of the heterogeneity of brain tissue on Magnetic Resonance Imaging (MRI) data in both healthy and diseased human brains, and to extract and quantify information on heterogeneity from the data. The project aims to develop novel mathematical and computational approaches to model the heterogeneity of the human brain. The project aims to identify new biomarkers for classifying differ ....New mathematical models for capturing heterogeneity of human brain tissue. This project aims to understand the impact of the heterogeneity of brain tissue on Magnetic Resonance Imaging (MRI) data in both healthy and diseased human brains, and to extract and quantify information on heterogeneity from the data. The project aims to develop novel mathematical and computational approaches to model the heterogeneity of the human brain. The project aims to identify new biomarkers for classifying different brain diseases, based on the extent of heterogeneity across different brain tissue. Results will be validated against extensive MRI scanning data of patients. This project aims to advance state-of-the-art techniques in human brain MRI data analysis.Read moreRead less