Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The p ....Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The proposed research seeks to delineate new races of the pathogen, to identify the histological and biochemical mechanisms by which resistance to Phytophthora root rot is expressed, and to identify new sources of host resistance. This proposed research will enable breeders, for the first time, to incorporate multiple types of resistance and against different races into new host varieties.Read moreRead less
Molecular approaches for the exploitation of genetic diversity in subterranean clover (Trifolium subterraneum L.) for profitable Australian farming systems. Subterranean clover is the most widely sown annual pasture legume species in southern Australia. It is native to the Mediterranean basin and has been sown over an estimated area of 22 million ha. This project will provide breeders with a focused core germplasm collection representing the range of genetic diversity of the around 8000 accessio ....Molecular approaches for the exploitation of genetic diversity in subterranean clover (Trifolium subterraneum L.) for profitable Australian farming systems. Subterranean clover is the most widely sown annual pasture legume species in southern Australia. It is native to the Mediterranean basin and has been sown over an estimated area of 22 million ha. This project will provide breeders with a focused core germplasm collection representing the range of genetic diversity of the around 8000 accessions in the original collection. The focused core collection will lead to more efficient and effective breeding of elite cultivars for sustainable and profitable farming systems to benefit wool, meat and grains industries.Read moreRead less
Turning sand into sheep feed - Lebeckia ambigua an agricultural perennial! This project aims to develop nitrogen-fixing legumes adapted to the changing climate. Nitrogen fixation from legumes is worth $3 billion to the Australian agricultural economy, but changing rainfall patterns threaten much of this. One solution is to transition pasture growth to a reliance on perennial plants, which are less affected by unseasonal rain. Lebeckia ambigua is an outstanding perennial legume to begin this chan ....Turning sand into sheep feed - Lebeckia ambigua an agricultural perennial! This project aims to develop nitrogen-fixing legumes adapted to the changing climate. Nitrogen fixation from legumes is worth $3 billion to the Australian agricultural economy, but changing rainfall patterns threaten much of this. One solution is to transition pasture growth to a reliance on perennial plants, which are less affected by unseasonal rain. Lebeckia ambigua is an outstanding perennial legume to begin this change, but its nitrogen fixation is compromised by nodulation failure caused by death of its symbiotic rhizobia. This project intends to improve the survival in acid and infertile soils of the unique rhizobial symbionts the research team has discovered for Lebeckia ambigua in South Africa.Read moreRead less
Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of th ....Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of the host-pathogen interactions. A successful outcome could contribute an additional 5-20% increase in crop yields (depending on the crop) through inherent resistance of crops to nematode pests. This would benefit rural communities and the national economy, and could also generate international royalties.Read moreRead less
Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important ....Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important nematode species and races, and to identify diagnostic proteins or epitopes. The diagnostic proteins will be used to generate specific monoclonal antibodies that will be incorporated into immunochemical 'Lateral Flow' devices. These will provide on-site tests to identify nematodes for growers and quarantine services. Read moreRead less
Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damag ....Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damage and persistence in groundwater, and biological control has had limited success. These problems are addressed in this project with development of synthetic plant resistance to nematodes, which will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basi ....Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basis of this discrepancy and to attempt to provide tools that circumvent the problem. It is expected the project will generate novel information on the environment in the plant experienced by the fungus.Read moreRead less
A novel method of broad-acre weed seedbank management using a naturally occuring germination stimulant. The discovery of a novel butenolide that promotes seed germination has potential to provide significant economic benefits for Australia's agricultural sector, providing a vehicle to move towards minimum-weed agricultural systems achieved through broad-acre stimulation of the weed seedbank. Our aim is for butenolide to promote uniform release of weed seed dormancy, increased germination, and gr ....A novel method of broad-acre weed seedbank management using a naturally occuring germination stimulant. The discovery of a novel butenolide that promotes seed germination has potential to provide significant economic benefits for Australia's agricultural sector, providing a vehicle to move towards minimum-weed agricultural systems achieved through broad-acre stimulation of the weed seedbank. Our aim is for butenolide to promote uniform release of weed seed dormancy, increased germination, and greater synchrony in early stage seedling growth and thus more effective knock-down following herbicide applications.Read moreRead less
Mechanisms and manipulation of seed dormancy maintenance in annual ryegrass and other weed species. A better understanding of seed dormancy in annual ryegrass will lead to a greater variety of management options for removal of this weed from cropping zones, focusing on diminishing the weed seed bank. Additionally, the knowledge gained from the study of ryegrass could be applied to native species in terms of optimising germination of difficult species and conservation of seeds of rare species.
Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across sout ....Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across southern Australia. Benefits include prevention of severe losses in canola from downy mildew, and more viable and sustainable production with less reliance upon fungicides. This research addresses the National Research Priority 'An Environmentally Sustainable Australia' and the Priority Goal of 'Transforming existing industries', and will particularly benefit southern Australian rural communities.Read moreRead less