Characterising structural variation in the canola genome. Characterising structural variation in the canola genome. This project aims to develop and apply genomic tools to identify and characterise structural genome variation in canola, a major Australian export crop, to better understand genome evolution and accelerate canola breeding. Advances in DNA sequencing revolutionise our understanding of crop genomes, their evolution and impact on the inheritance on agronomic traits. Variation of genom ....Characterising structural variation in the canola genome. Characterising structural variation in the canola genome. This project aims to develop and apply genomic tools to identify and characterise structural genome variation in canola, a major Australian export crop, to better understand genome evolution and accelerate canola breeding. Advances in DNA sequencing revolutionise our understanding of crop genomes, their evolution and impact on the inheritance on agronomic traits. Variation of genome structure between individuals could be important in the inheritance of important agronomic traits. Recent advances in technology permit the detailed characterisation of structural variation on a previously unfeasible scale. Anticipated outcomes are enhanced global food security, supporting rural Australian economies, and accelerating the improvement of other major crops.Read moreRead less
Defining the Brassica pan-genome and establishing methods for gene conversion based crop improvement. Gene content varies between individual varieties. The project aims to apply novel genomic tools to identify and characterise the fixed and variable gene content in the important crop canola and use this to understand genome evolution as well as develop tools to accelerate canola breeding. The project team have developed and used a high-resolution genotyping approach to demonstrate that gene conv ....Defining the Brassica pan-genome and establishing methods for gene conversion based crop improvement. Gene content varies between individual varieties. The project aims to apply novel genomic tools to identify and characterise the fixed and variable gene content in the important crop canola and use this to understand genome evolution as well as develop tools to accelerate canola breeding. The project team have developed and used a high-resolution genotyping approach to demonstrate that gene conversions, short recombination events which lead to the non-reciprocal exchange of genomic regions during meiosis, are abundant in crop genomes. The project aims to develop methods and resources to characterise gene conversion in canola and establish a basis for gene conversion based crop improvement.Read moreRead less
Brassica genome organisation and evolution: unlocking the potential of using genome-specific repetitive elements for crop improvement. Introgression of chromosome segments from related Brassica species provides an opportunity to develop locally adapted varieties with improved agronomic and quality traits. There is a need to understand Brassica genome organisation and how this information can be used for enhancing the efficiency of cultivar development. Dispersed and tandem repetitive DNA sequen ....Brassica genome organisation and evolution: unlocking the potential of using genome-specific repetitive elements for crop improvement. Introgression of chromosome segments from related Brassica species provides an opportunity to develop locally adapted varieties with improved agronomic and quality traits. There is a need to understand Brassica genome organisation and how this information can be used for enhancing the efficiency of cultivar development. Dispersed and tandem repetitive DNA sequences provide valuable information on the organisation and evolution of plant chromosomes. Methods for monitoring chromosome segment transfer across Brassica species will be developed based on detecting and quantifying genome-specific repetitive DNA sequences. Australian Brassica improvement programs could benefit from this research by adopting methods to detect chromosome segment transfer during interspecific hybridisation.Read moreRead less
Exploiting the Arabidopsis genome sequence as a molecular 'toolbox' for Brassica improvement. Australia's position as a major exporter of canola (Brassica napus) is under threat from genetic improvements in yield and quality being made by our international competitors. We will identify genes from Arabidopsis (the 'tool-box') that will be used to increase the speed of selection of new canola varieties with improved oleic acid content, disease resistance, and agronomic traits such as early flower ....Exploiting the Arabidopsis genome sequence as a molecular 'toolbox' for Brassica improvement. Australia's position as a major exporter of canola (Brassica napus) is under threat from genetic improvements in yield and quality being made by our international competitors. We will identify genes from Arabidopsis (the 'tool-box') that will be used to increase the speed of selection of new canola varieties with improved oleic acid content, disease resistance, and agronomic traits such as early flowering and cold tolerance. Genome similarity between Arabidopsis and canola will be exploited to map specific genes from Arabidopsis directly into canola. Based on this knowledge, we will develop gene-specific molecular markers for rapid selection of Australian-adapted canola varieties.Read moreRead less
Identification of Traits and Function by Genomic Matching. Differences between individuals are largely inherited and therefore encoded within the DNA. The challenge is to develop practical means of detecting these differences irrespective of whether they are observable as a phenotype.
Here we focus on livestock. For example, most Australian cattle are horned rather than polled. The inheritance is relatively simple but there is still no DNA test to detect the recessive horning gene.
T ....Identification of Traits and Function by Genomic Matching. Differences between individuals are largely inherited and therefore encoded within the DNA. The challenge is to develop practical means of detecting these differences irrespective of whether they are observable as a phenotype.
Here we focus on livestock. For example, most Australian cattle are horned rather than polled. The inheritance is relatively simple but there is still no DNA test to detect the recessive horning gene.
The genomic matching technique is an in-house patented procedure for identifying such DNA differences. If successful, our test will assist industry to eliminate horning and thereby painful dehorning whilst reducing damage to workers and product.
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Establishment of the Australian Cane Toad Genome Program. The Cane Toad is one of Australia's greatest environmental menaces, and is in the top 100 of the "World's Worst invader species". Over a billion toads infest Northern Australia, and they will soon invade WA and move further into NSW. Their poisons and voracious appetite could make many native mammals, birds and reptiles extinct. The only possibility to eradicate the Toad is by biological control, but there is no known control agent. We wi ....Establishment of the Australian Cane Toad Genome Program. The Cane Toad is one of Australia's greatest environmental menaces, and is in the top 100 of the "World's Worst invader species". Over a billion toads infest Northern Australia, and they will soon invade WA and move further into NSW. Their poisons and voracious appetite could make many native mammals, birds and reptiles extinct. The only possibility to eradicate the Toad is by biological control, but there is no known control agent. We will identify the 'Toad's Achilles' heel' against which control agents can be developed. We can do this by identifying every Toad gene. This project forms the first step to this goal by establishing the Australian Cane Toad Genome Program. Toad control will help preserve Australia's unique natural heritage.Read moreRead less
New biotech methods for crop quality assurance. Quality assurance of crop products is a key for Australia to be competitive in the world marketplace. The power of molecular diagnostics has not been applied to this important but neglected part of the produce handling chain. In this project research will be undertaken that will lead to low cost on site assays to test for variety preservation, contamination, and presence of pests and diseases. It employs the tools of genomics and proteomics to p ....New biotech methods for crop quality assurance. Quality assurance of crop products is a key for Australia to be competitive in the world marketplace. The power of molecular diagnostics has not been applied to this important but neglected part of the produce handling chain. In this project research will be undertaken that will lead to low cost on site assays to test for variety preservation, contamination, and presence of pests and diseases. It employs the tools of genomics and proteomics to provide basic understanding of processes which can be developed into cost effective analyses for practical use by industry to ensure quality assurance.Read moreRead less
Expanding the gene pool of canola (Brassica napus) by introgressing valuable genes from related species. Canola is a high value export crop from Australia, and an important rotational crop which improves sustainability of agriculture through a disease and weed break for cereal crops. While breeders have improved quality, disease resistance and adaptation of canola to Australian conditions over the past 30 years, this has reduced genetic variation to dangerously low levels. Wide crossing with d ....Expanding the gene pool of canola (Brassica napus) by introgressing valuable genes from related species. Canola is a high value export crop from Australia, and an important rotational crop which improves sustainability of agriculture through a disease and weed break for cereal crops. While breeders have improved quality, disease resistance and adaptation of canola to Australian conditions over the past 30 years, this has reduced genetic variation to dangerously low levels. Wide crossing with drought tolerant Brassica carinata (Ethiopean mustard) will help to alleviate this problem. New uses of biotechnology, combined with molecular genetics, will help to overcome species barriers to introduce useful new genes into canola for Australian canola breeders.Read moreRead less
Genomic Synteny in Legumes; Application to Crop Breeding. Synteny is defined as the degree of colinearity between the order of orthologous genes in chromosomes of related species. New data suggests that legumes share widespread synteny. Extensive genomic resources are being accumulated for the model legume, Medicago truncatula including the full genome sequence. We propose to use these resources to uncover patterns of synteny between M. truncatula and the cultivated legumes chickpeas, lupins, ....Genomic Synteny in Legumes; Application to Crop Breeding. Synteny is defined as the degree of colinearity between the order of orthologous genes in chromosomes of related species. New data suggests that legumes share widespread synteny. Extensive genomic resources are being accumulated for the model legume, Medicago truncatula including the full genome sequence. We propose to use these resources to uncover patterns of synteny between M. truncatula and the cultivated legumes chickpeas, lupins, faba-beans, lentils and lucerne. In addition to revealing patterns of chromosomal evolution in this large plant family, we will generate genetic maps and molecular markers for use in practical plant breeding of these important crops.Read moreRead less
Vulnerable families: A study of the impact of parental offending and incarceration on children's developmental outcomes. The limited evidence available suggests that parental incarceration severely compromises the development and well-being of children and increases the risk of delinquency. The financial and personal costs to the community of individuals embarking on an offending career are substantial. Significant cost-savings and prevention of victimisation could result from effectively target ....Vulnerable families: A study of the impact of parental offending and incarceration on children's developmental outcomes. The limited evidence available suggests that parental incarceration severely compromises the development and well-being of children and increases the risk of delinquency. The financial and personal costs to the community of individuals embarking on an offending career are substantial. Significant cost-savings and prevention of victimisation could result from effectively targeting this high-risk population. This research will identify where and when scarce government resources should be targeted. Appropriately timed programs can deliver a range of long-term benefits for children, families and communities. The research will also investigate the reintegration of prisoners into their families, with the aim of reducing prisoner recidivism.Read moreRead less