Photodissociation mass spectrometry for lipidome analysis. This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisatio ....Photodissociation mass spectrometry for lipidome analysis. This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisation of the, potentially, tens of thousands of individual molecular lipid species that may be present within a sample of interest. This project will develop and optimise novel, ultraviolet photodissociation-tandem mass spectrometry methods which will be integrated within an automated lipidome analysis workflow, to enable comprehensive global lipidome profiling and to reveal the structural diversity of lipids involved in fundamental cellular signalling processes.Read moreRead less
Does phonological awareness help children learn to read? An almost universally-accepted view in the field of reading acquisition is that phonological awareness, or the ability to perceive and manipulate speech sounds, causes a child to be good at learning to read. We argue that, despite the voluminous literature on this issue, it has not been conclusively established that such a causal link exists. To do so requires a project, proposed here, in which completely pre-literate children are selec ....Does phonological awareness help children learn to read? An almost universally-accepted view in the field of reading acquisition is that phonological awareness, or the ability to perceive and manipulate speech sounds, causes a child to be good at learning to read. We argue that, despite the voluminous literature on this issue, it has not been conclusively established that such a causal link exists. To do so requires a project, proposed here, in which completely pre-literate children are selected, their phonological awareness measured, and its relationship with subsequent literacy acquisition followed. Settling this issue will have significant consequences for both theory and practice in reading acquisition and dyslexia.Read moreRead less
The RGG/RG motif as an RNA chaperone: advancing CRISPR-Cas RNA technology. This project investigates the way in which protein molecules interact effectively with RNA molecules and also aims to enhance the CRISPR-Cas13a system for RNA detection. Innovative approaches will be used to test the role of a particular protein motif, called the RGG/RG motif, in remodelling RNA structure and enhancing the Cas13a protein. This knowledge is expected to shift our understanding of protein-RNA interactions th ....The RGG/RG motif as an RNA chaperone: advancing CRISPR-Cas RNA technology. This project investigates the way in which protein molecules interact effectively with RNA molecules and also aims to enhance the CRISPR-Cas13a system for RNA detection. Innovative approaches will be used to test the role of a particular protein motif, called the RGG/RG motif, in remodelling RNA structure and enhancing the Cas13a protein. This knowledge is expected to shift our understanding of protein-RNA interactions that are fundamental to almost every aspect of cell biology. The project is intended to benefit Australia through contributing to fundamental knowledge in the field, facilitating the development of new CRISPR-Cas biotechnologies for RNA detection and through the training of young researchers in frontier technologies. Read moreRead less
Determinants of Audio-Visual effects in degraded and non-degraded speech. Seeing a speaker's face can affect the perception of their speech in a number of ways. This project proposes a detailed comparison of factors that affect Audio-Visual (AV) facilitation of degraded speech detection and identification. Detection-based tasks should be more sensitive to signal based correlations whereas identification-based effects more sensitive to complementary information. The significance of the current pr ....Determinants of Audio-Visual effects in degraded and non-degraded speech. Seeing a speaker's face can affect the perception of their speech in a number of ways. This project proposes a detailed comparison of factors that affect Audio-Visual (AV) facilitation of degraded speech detection and identification. Detection-based tasks should be more sensitive to signal based correlations whereas identification-based effects more sensitive to complementary information. The significance of the current proposal is that it offers both a strategy and a connected series of experiments for determining key behavioural constraints on AV speech integration. Understanding AV interactions will build links between neurophysiological processes and coherent perception and have important implications for AV application.Read moreRead less
Developing a Unified Theory of Episodic Memory. This project aims to develop a model of episodic memory and to apply the model to both adult and child development data. Unlike current approaches, the model is expected to address multiple memory tasks including item recognition, associative recognition, source recognition and cued recall, and also aims to address reaction time data, allowing different sources of interference causing forgetting in adults to be identified. By addressing both encodi ....Developing a Unified Theory of Episodic Memory. This project aims to develop a model of episodic memory and to apply the model to both adult and child development data. Unlike current approaches, the model is expected to address multiple memory tasks including item recognition, associative recognition, source recognition and cued recall, and also aims to address reaction time data, allowing different sources of interference causing forgetting in adults to be identified. By addressing both encoding and retrieval processes, the model can assess how changes in different sources of interference modulate performance through the trajectory of early development. Hierarchical Bayesian estimation aims to enable a simultaneous account of multiple tasks and support future deployment in applied contexts.Read moreRead less
Stochastic, Neurally-Plausible Models for Selective Attention and Decision Making. An understanding of the basic cognitive processes involved in attention and decision making is the goal of international research effort in a number of disciplines. The benefits expected from such understanding include improvements in the diagnosis and treatment of a variety of cognitive deficits and improved occupational safety and efficiency in settings involving interaction with complex systems, such air traffi ....Stochastic, Neurally-Plausible Models for Selective Attention and Decision Making. An understanding of the basic cognitive processes involved in attention and decision making is the goal of international research effort in a number of disciplines. The benefits expected from such understanding include improvements in the diagnosis and treatment of a variety of cognitive deficits and improved occupational safety and efficiency in settings involving interaction with complex systems, such air traffic control, airline cockpits, motor vehicles, and process management. By developing mathematical models of these processes and the neural mechanisms that underlie them, this project will contribute to this understanding. It will also provide international postdoctoral opportunties for Australian-trained Ph.D. graduates.Read moreRead less
Understanding the relationship between child maltreatment and language competence: An evidential interviewing perspective. This research aims to develop the first comprehensive and controlled examination of the oral language profiles of maltreated children and how these impact performance in an investigative interview paradigm. This examination is essential for developing strategies for improving the elicitation of evidence from child abuse witnesses. By improving child witness evidence, this re ....Understanding the relationship between child maltreatment and language competence: An evidential interviewing perspective. This research aims to develop the first comprehensive and controlled examination of the oral language profiles of maltreated children and how these impact performance in an investigative interview paradigm. This examination is essential for developing strategies for improving the elicitation of evidence from child abuse witnesses. By improving child witness evidence, this research will improve the prosecution and conviction rates of child abuse offences, which will have a deterrent effect on potential offenders. Finally, this research will reduce the stress of witnesses involved in the legal process and provide better screening of children in need of specialised language intervention.Read moreRead less
An Integrated Theory of Attention and Decision Making. Biologically, the fundamental computational task carried out by the human brain is the translation of perception into action. To perform this translation, attentional processes select relevant stimuli from the environment and decision processes then identify the selected stimuli. This project develops an integrated theory of attention and decision making in simple visual tasks, which will help us understand how these tasks are carried out i ....An Integrated Theory of Attention and Decision Making. Biologically, the fundamental computational task carried out by the human brain is the translation of perception into action. To perform this translation, attentional processes select relevant stimuli from the environment and decision processes then identify the selected stimuli. This project develops an integrated theory of attention and decision making in simple visual tasks, which will help us understand how these tasks are carried out in the brain. Understanding how these tasks are performed biologically will aid in the design of autonomous information-gathering and decision-making agents whose actions simulate human behaviour. Read moreRead less
The Stochastic Dynamics of Visual Attention. This project develops and tests a class of mathematical models of how information is represented statistically in the human visual system and how that representation is modified by selective attention. These models synthesise current theories about how the visual system encodes information and how people make judgements on the basis of that information. The domain of application of the existing class of models will be extended to allow them to predi ....The Stochastic Dynamics of Visual Attention. This project develops and tests a class of mathematical models of how information is represented statistically in the human visual system and how that representation is modified by selective attention. These models synthesise current theories about how the visual system encodes information and how people make judgements on the basis of that information. The domain of application of the existing class of models will be extended to allow them to predict performance in more complex settings than has hitherto been the case. A program of experimental studies will test the models by measuring human performance in response to computer-generated stimuli.
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Spectroscopic investigations into nano-scale drug interactions and molecular processes in single living cells and isolated molecules. This project will develop micro-spectroscopy and nano-spectroscopy molecular imaging based techniques to spatially locate and determine the bonding sites of a new range of chemotherapeutic drugs designed to treat cancer and malaria. This project will adopt a translational approach looking first at drug interactions directly with molecules such as DNA. Secondly, in ....Spectroscopic investigations into nano-scale drug interactions and molecular processes in single living cells and isolated molecules. This project will develop micro-spectroscopy and nano-spectroscopy molecular imaging based techniques to spatially locate and determine the bonding sites of a new range of chemotherapeutic drugs designed to treat cancer and malaria. This project will adopt a translational approach looking first at drug interactions directly with molecules such as DNA. Secondly, investigating these interactions in living cells and finally applying the technology to tissue samples. The outcome of this research will be new drug screening technologies and methodologies to address two of the most devastating diseases to afflict human kind, offering hope to the countless millions suffering from these diseases.Read moreRead less