Industrial biotechnology for improved manufacture of medicinal alkaloids. Industrial biotechnology for improved manufacture of medicinal alkaloids. This project aims to develop a new semi-synthetic route for the manufacture of Codeine from Thebaine. Codeine is manufactured by the chemical conversion of poppy-derived Morphine, which is low yielding and environmentally unfriendly. This project will investigate and optimise two key enzymes in the biosynthetic pathway to Codeine and develop an innov ....Industrial biotechnology for improved manufacture of medicinal alkaloids. Industrial biotechnology for improved manufacture of medicinal alkaloids. This project aims to develop a new semi-synthetic route for the manufacture of Codeine from Thebaine. Codeine is manufactured by the chemical conversion of poppy-derived Morphine, which is low yielding and environmentally unfriendly. This project will investigate and optimise two key enzymes in the biosynthetic pathway to Codeine and develop an innovative bioreactor to achieve high yield. It will research industrial biotransformations, providing a greener and cheaper process for increased global access to Codeine. Australian opiate manufacturing is expected to benefit from value adding and increased flexibility introduced by a biotechnology approach.Read moreRead less
The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cance ....The Other Side: Long-distance Feedback and Cross-talk in the Arabidopsis Branching Gene Network. Shoot structure in nearly all plants impacts on water relations, yield and sustainability. Isolation of novel genes and plant hormone pathways that affect shoot structure should allow us to capture IP for Australia and enhance plant improvement and sustainability. One of the pathways we will investigate produces glucosinolates, small plant-specific molecules that have considerable value as anti-cancer agents in humans. Flavonoids, another pathway regulated in our shoot branching lines, also benefit human health. Graduate students and postdoctoral researchers will gain skills in research management, design, ethics and experimental methods pertinent to the growing biotechnology industry in Australia.Read moreRead less
Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes o ....Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes of this project are cost-effective and strongly enhanced approaches for biopharmaceuticals recovery, thereby providing significant benefits to accelerate research and development in early stage discovery and manufacture of biologics, therapeutic proteins and vaccines.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE160101570
Funder
Australian Research Council
Funding Amount
$352,000.00
Summary
Inducible hyper-expression as a tool for metabolic engineering. This project aims to develop methods to improve plant natural products yields and diversify the products manufactured from a wide array of plant species. Valuable plant metabolites, including chemotherapeutics and opiates, are usually synthesised in minute amounts and can be so complex they can’t be chemically manufactured, limiting their use and affordability. This project aims to develop an inducible system that confers hyper-elev ....Inducible hyper-expression as a tool for metabolic engineering. This project aims to develop methods to improve plant natural products yields and diversify the products manufactured from a wide array of plant species. Valuable plant metabolites, including chemotherapeutics and opiates, are usually synthesised in minute amounts and can be so complex they can’t be chemically manufactured, limiting their use and affordability. This project aims to develop an inducible system that confers hyper-elevated levels of gene expression and independent control of multiple genes involved in metabolite synthesis in the same host. This technology is expected to enable flexible multi-trait bio-factories to be developed.Read moreRead less
The use of molecular sponges to inhibit small Ribonucleic acid activity in plants. The deletion of gene activity is the most powerful way to understand gene function; however for genes encoding small Ribonucleic acids (RNAs) no current methodology can efficiently achieve this. Here, we aim to develop a gene silencing technology for small RNA encoding genes, which can be utilised to determine their function and used for biotechnological applications.
Evolution of halophytes: a phyloinformatic approach to understanding and exploiting the traits underlying salt-tolerance in plants. Salinity is an increasing burden on the Australian economy & environment, with >2 million ha of salt-affected land, at an annual cost to agriculture over $187 million. One solution is to exploit naturally salt-tolerant plants to increase productive agricultural land and restore salt-affected environments. To do this, we must increase basic knowledge of the diversity ....Evolution of halophytes: a phyloinformatic approach to understanding and exploiting the traits underlying salt-tolerance in plants. Salinity is an increasing burden on the Australian economy & environment, with >2 million ha of salt-affected land, at an annual cost to agriculture over $187 million. One solution is to exploit naturally salt-tolerant plants to increase productive agricultural land and restore salt-affected environments. To do this, we must increase basic knowledge of the diversity & distribution of salt-tolerance. This project is the first to use DNA sequences from thousands of species to understand the evolution of salt-tolerance in order to provide the foundation for the development of new crop varieties, selection of species that can be developed for bioremediation, and identification of traits that will be profitable targets for breeding programs. Read moreRead less
In-Vitro Production of Baculovirus Biopesticides - A Systems Biology Approach. This project has the potential to develop an in-vitro production process that can produce large quantities of Baculoviruses at costs comparable to selective chemicals. This could transform agriculture allowing farmers to choose an insect control option that is both safe and efficacious to use as well as economically and environmentally superior to chemicals, and less controversial than transgenic plants. This outcome ....In-Vitro Production of Baculovirus Biopesticides - A Systems Biology Approach. This project has the potential to develop an in-vitro production process that can produce large quantities of Baculoviruses at costs comparable to selective chemicals. This could transform agriculture allowing farmers to choose an insect control option that is both safe and efficacious to use as well as economically and environmentally superior to chemicals, and less controversial than transgenic plants. This outcome would enhance Australia's reputation in the animal cell technology field (related technology is used to produce protein pharmaceutical products), and will earn significant export dollars through licensing out of the technology or through large scale manufacturing and export of the product itself. Read moreRead less
Effect of Chemo-Mechanical Grinding on Surface Integrity of Single Crystal Silicon Substrates. Silicon substrates or wafers are extensively used in electronic and optic/photonic industries. A long-time standing problem in silicon wafer machining is the surface and subsurface damage induced by machining. This may significantly affect the mechanical, optical and electronic characteristics of wafer-based components. The issue becomes increasingly more critical as the application of silicon wafers i ....Effect of Chemo-Mechanical Grinding on Surface Integrity of Single Crystal Silicon Substrates. Silicon substrates or wafers are extensively used in electronic and optic/photonic industries. A long-time standing problem in silicon wafer machining is the surface and subsurface damage induced by machining. This may significantly affect the mechanical, optical and electronic characteristics of wafer-based components. The issue becomes increasingly more critical as the application of silicon wafers is extending further as structural components. The research outcomes will contribute an improved understanding of Chemo Mechanical Grinding process and help to develop innovative technologies for silicon industries.Read moreRead less
Skid mounted process for on-demand acetylene production. Skid mounted process for on-demand acetylene production. This project aims to develop a new, cheaper, safer and easier process for providing acetylene for the metal fabrication and construction industries. Mains gas (methane) will be converted into acetylene, on-site and on-demand, using a highly compact, transportable skid-based gas generator that is self-operating with a single touch ON-OFF system. This should remove the need to deliver ....Skid mounted process for on-demand acetylene production. Skid mounted process for on-demand acetylene production. This project aims to develop a new, cheaper, safer and easier process for providing acetylene for the metal fabrication and construction industries. Mains gas (methane) will be converted into acetylene, on-site and on-demand, using a highly compact, transportable skid-based gas generator that is self-operating with a single touch ON-OFF system. This should remove the need to deliver bottled acetylene to metal fabrication works, eliminating road transport and storage risks. There is a high potential for export of the plants to countries like China and India that use a calcium carbide-based process as a distributed acetylene source.Read moreRead less
Transforming Australian bio-based industries through multiscale modelling. Agricultural and forestry biomass can be converted into feedstocks for production of biofuels and biomaterials via synthetic biology. A key challenge is the complex biomass microstructure renders it highly resistant to conversion, and pretreatment is crucial for enhancing process efficiency. Micro-CT imaging will enable particle characterisation and identification of changes in the fibre composition during pretreatment. T ....Transforming Australian bio-based industries through multiscale modelling. Agricultural and forestry biomass can be converted into feedstocks for production of biofuels and biomaterials via synthetic biology. A key challenge is the complex biomass microstructure renders it highly resistant to conversion, and pretreatment is crucial for enhancing process efficiency. Micro-CT imaging will enable particle characterisation and identification of changes in the fibre composition during pretreatment. This information will be used to create a virtual biomass particle model for an in silico investigation to inform optimal process design. The framework will transform the way biomass is processed, contributing to the growth of the Australian bio-manufacturing industry by making it more productive, profitable and sustainable.Read moreRead less