Contribution of comammox bacteria to soil nitrification. This project aims to understand the diversity, activity and environmental relevance of comammox bacteria, the newly-discovered complete nitrifiers, in Australian soils, and to evaluate their relative contributions to nitrification processes compared to other canonical nitrifying prokaryotes. Nitrogen transformations are pivotal microbial processes, with nitrification largely responsible for nitrogen losses through nitrous oxide emissions a ....Contribution of comammox bacteria to soil nitrification. This project aims to understand the diversity, activity and environmental relevance of comammox bacteria, the newly-discovered complete nitrifiers, in Australian soils, and to evaluate their relative contributions to nitrification processes compared to other canonical nitrifying prokaryotes. Nitrogen transformations are pivotal microbial processes, with nitrification largely responsible for nitrogen losses through nitrous oxide emissions and nitrate leaching in the terrestrial ecosystems. The expected outcomes will develop new knowledge on the comammox bacteria and provide novel insights into refined strategies to manipulate nitrification processes for improved nitrogen use efficiency and sustainable agricultural management.Read moreRead less
Interaction of Cryptosporidium lifecycle stages with aquatic biofilm communities. Cryptosporidium is the most common non-viral cause of diarrhoeal disease in humans worldwide, and of increasing significance as a cause of disease in livestock and wildlife. It is one of the most significant waterborne pathogens and a major challenge to the provision of safe drinking water by water utilities. Biofilms are a poorly studied component of Cryptosporidium's ecosystem, and can act as an environmental res ....Interaction of Cryptosporidium lifecycle stages with aquatic biofilm communities. Cryptosporidium is the most common non-viral cause of diarrhoeal disease in humans worldwide, and of increasing significance as a cause of disease in livestock and wildlife. It is one of the most significant waterborne pathogens and a major challenge to the provision of safe drinking water by water utilities. Biofilms are a poorly studied component of Cryptosporidium's ecosystem, and can act as an environmental reservoir of the parasite in water storages and pipes and an unpredictable source of water contamination. This project will investigate the nature of this reservoir and factors that support the parasite's survival with a view to providing information of value in limiting the public health significance of the biofilm reservoir.Read moreRead less
Causes of and Cures for Microbiological Foams in Activated Sludge Wastewater Treatment Plants. Little is known of the true diversity of the bacteria causing foaming in activated sludge systems, or why and how they cause these foams. This application seeks funds for an interdisciplinary approach to address these issues, involving input from microbiologists and surface chemists and physicists. Molecular techniques will be used to determine precisely the foaming bacterial communities and their phys ....Causes of and Cures for Microbiological Foams in Activated Sludge Wastewater Treatment Plants. Little is known of the true diversity of the bacteria causing foaming in activated sludge systems, or why and how they cause these foams. This application seeks funds for an interdisciplinary approach to address these issues, involving input from microbiologists and surface chemists and physicists. Molecular techniques will be used to determine precisely the foaming bacterial communities and their physiology, while their surface chemistry and organisation will be studied to understand the mechanisms involved in foaming with the intention of developing control strategies for this global problem suitable for large scale application.Read moreRead less
Metagenomics and the genetic basis of ecology and evolution of communities - complex microbial communities in industrial processes as excellent paradigms. Benefits accrue on two fronts: the international reputation of Australian science in contributing significantly to two new, challenging and highly topical questions in ecology and evolution, and in pioneering a route to better efficiency and control of an important industrial process. Wastewater is rich in organic phosphorus that is damaging t ....Metagenomics and the genetic basis of ecology and evolution of communities - complex microbial communities in industrial processes as excellent paradigms. Benefits accrue on two fronts: the international reputation of Australian science in contributing significantly to two new, challenging and highly topical questions in ecology and evolution, and in pioneering a route to better efficiency and control of an important industrial process. Wastewater is rich in organic phosphorus that is damaging to the environment if untreated, but current understanding enables only rudimentary control of the microbial communities that are the basis of the treatment process. The work will provide unprecedented insight into the mechanisms underpinning the dynamics of phosphorus absorbing microbes in industrial facilities by integrating from gene-to-ecosystem.Read moreRead less
Transition from phosphate mining to an economically, environmentally and socially viable agricultural industry on Christmas Island. The main industry on Christmas Island is mining of rock phosphate, but supplies will run out between 2025 and 2030. Consequently, there is an urgent and compelling need to develop other economic industries to support the resident island population by the time mining ceases. This project aims to utilise the project team’s extensive knowledge on legumes, nitrogen fixi ....Transition from phosphate mining to an economically, environmentally and socially viable agricultural industry on Christmas Island. The main industry on Christmas Island is mining of rock phosphate, but supplies will run out between 2025 and 2030. Consequently, there is an urgent and compelling need to develop other economic industries to support the resident island population by the time mining ceases. This project aims to utilise the project team’s extensive knowledge on legumes, nitrogen fixing bacteria and plant growth promoting bacteria to establish the basis for a viable agricultural industry on the Island. The project aims to provide: reliable and sustainable food sources for the local population; potential food export opportunities to southeast Asia; environmental benefits from the improvement of soils; and economic development of allied agricultural industries.Read moreRead less
Sodium homeostasis and the molecular basis for neurotoxin production by bacteria and algae. An understanding of the physiology of saxitoxin-producing microorgansims in response to salt stress is critical for the prevention of toxic blooms and for risk assessment of contaminated water bodies. This is nowhere more relevant than in the depleted and increasingly saline water resources of inland Australia. This project will develop genetic tests to assay for saxitoxin-producers and to monitor toxin p ....Sodium homeostasis and the molecular basis for neurotoxin production by bacteria and algae. An understanding of the physiology of saxitoxin-producing microorgansims in response to salt stress is critical for the prevention of toxic blooms and for risk assessment of contaminated water bodies. This is nowhere more relevant than in the depleted and increasingly saline water resources of inland Australia. This project will develop genetic tests to assay for saxitoxin-producers and to monitor toxin production in response to the environment, representing an easier, more economic and ethical alternative to current tests. The market for this type of predictive test includes environmental, anti-bioterrorism and fishery organisations. These genes will also allow the bioengineering of novel therapeutic drugs based on neuroactive alkaloids.Read moreRead less
Carbon costs of plant nutrient and water uptake. This project aims to investigate how much carbon plants need to invest belowground in return for water and nutrients. By using economic principles of supply and demand the project will quantify carbon expenditure for water and nutrients in grasslands and crops under different climate and land management scenarios. This project will use triple and quadruple isotope labelling techniques and explore the dependency of carbon investment on plant-microb ....Carbon costs of plant nutrient and water uptake. This project aims to investigate how much carbon plants need to invest belowground in return for water and nutrients. By using economic principles of supply and demand the project will quantify carbon expenditure for water and nutrients in grasslands and crops under different climate and land management scenarios. This project will use triple and quadruple isotope labelling techniques and explore the dependency of carbon investment on plant-microbial interactions and availability of belowground resources. Expected outcomes include new knowledge to build a universal framework about plant carbon-water-nutrient economics. This will benefit global carbon cycling models and efforts to increase nutrient and water use efficiencies in agricultural crops.Read moreRead less
Development and implementation of biodiversity information for sustainable management of South Australian groundwater. Clean potable water is one of the most important resources for human health and a successful economy. Increasingly, subterranean aquifers are used for storage and recovery of water. These aquifers contain dynamic ecosystems, but little is known about species composition or about the importance of the presence of various species for water quality. We will use the latest laborator ....Development and implementation of biodiversity information for sustainable management of South Australian groundwater. Clean potable water is one of the most important resources for human health and a successful economy. Increasingly, subterranean aquifers are used for storage and recovery of water. These aquifers contain dynamic ecosystems, but little is known about species composition or about the importance of the presence of various species for water quality. We will use the latest laboratory techniques and DNA identification methods to provide a template for determining ground water diversity and food web dynamics throughout Australia. This project will lead to a better understanding of how to manage ground water in a sustainable manner.Read moreRead less
Maintenance of Australia's soil resource - water, microbial diversity and function. Water availability is the major limitation to biological activity in semi-arid regions of Australia. We aim to quantify the dynamic relationships between organic matter cycling, microbial diversity and function in relation to seasonality, drought and land management. This will lead to a comprehensive understanding of how water mediates the diversity of soil organisms and their associated functions. This is of sp ....Maintenance of Australia's soil resource - water, microbial diversity and function. Water availability is the major limitation to biological activity in semi-arid regions of Australia. We aim to quantify the dynamic relationships between organic matter cycling, microbial diversity and function in relation to seasonality, drought and land management. This will lead to a comprehensive understanding of how water mediates the diversity of soil organisms and their associated functions. This is of specific relevance to maintaining biodiversity within the unique soil ecosystems that have developed under Australian climatic conditions.Read moreRead less
The characterisation of wastewater distribution patterns for the production of decision support systems for pathogenic risk analysis in water catchments. The project will aim to collate data and map base-level distribution patterns of molecular and microbial markers of human sewage and natural/agricultural contamination with reference to diurnal and seasonal fluctuations. These data will then be used to develop decision support systems that predict contaminant dispersion and identify high-risk c ....The characterisation of wastewater distribution patterns for the production of decision support systems for pathogenic risk analysis in water catchments. The project will aim to collate data and map base-level distribution patterns of molecular and microbial markers of human sewage and natural/agricultural contamination with reference to diurnal and seasonal fluctuations. These data will then be used to develop decision support systems that predict contaminant dispersion and identify high-risk contamination sources. The diurnal collection of data is expected to show significant variations in the measured parameters associated with time of day, light intensity and temperature. Mapping of the sewage and nutrient loads throughout the year will provide base line data for identifying potential hotspots for targeting system upgrades or improved management programs.Read moreRead less