Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100114
Funder
Australian Research Council
Funding Amount
$560,000.00
Summary
High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as ....High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as well as validation of next generation sequencing data. The information generated is crucial to advancing knowledge in important research fields including infection and immunity, regenerative medicine, immune responses, biomarker discovery, drug discovery, biotechnology and agriculture.Read moreRead less
Characterisation of tumour variants of Devil Facial Tumour Disease. This project will take a new approach to cancer research by studying the evolution of Devil Facial Tumour Disease. The results will directly contribute to the conservation management of the Tasmanian devil, as well as generating new information on tumour growth, metastasis and emergence of resistance.
Epigenetics, environment, and evolution. This project will aim to understand how biological information can exist and be passed from one generation to the next without being encoded in the gene sequence, and also how our early environment can modify this so-called "epigenetic" information to alter disease risk.
Beyond the genome: unravelling the intricacies of epigenetic regulation using the honey bee model. Epigenetic mechanisms, such as DNA methylation, provide the interface between genome and environment. Abnormalities in epigenetic regulation lead to cancer and other diseases. The project will be using the alternative phenotypes in honeybees, fertile queens and sterile workers, to understand how dietary factors control conditional gene expression by methylation
Bacterial innovation and evolution: Molecular prospecting by targeting integrons and gene cassettes. Bacteria can respond rapidly to environmental change by acquiring new genes via lateral gene transfer. A DNA element called the integron can capture, mobilise and express genes, thereby playing a role in the transfer process. We have discovered that integrons are surprisingly abundant in the environment and are associated with a hitherto unsuspected diversity of novel genes. In this study we will ....Bacterial innovation and evolution: Molecular prospecting by targeting integrons and gene cassettes. Bacteria can respond rapidly to environmental change by acquiring new genes via lateral gene transfer. A DNA element called the integron can capture, mobilise and express genes, thereby playing a role in the transfer process. We have discovered that integrons are surprisingly abundant in the environment and are associated with a hitherto unsuspected diversity of novel genes. In this study we will assess the diversity of environmental integrons and examine their contribution to bacterial evolution. Further, we aim to use integron systems to prospect for novel genes and contract new enzyme pathways by directed evolution.
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Discovery Early Career Researcher Award - Grant ID: DE120100723
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
The inheritance of epigenetic information in mammals. This project aims to understand how biological information can be passed from one generation to the next without being encoded in the genes. This may explain questions as diverse as why twins look subtly different and why some families are more likely than others to suffer disease.
Charting the human epi-transcriptome. This project aims to use Oxford nanopore technologies and phage display technologies, to obtain quantitative, single-nucleotide resolution maps for any RNA modification of choice. This will allow systematic mapping of RNA modifications for which we currently lack transcriptome-wide maps, as well as investigate the roles, regulation and impact of RNA modifications in proper cellular functioning and cell differentiation. The project will provide significant be ....Charting the human epi-transcriptome. This project aims to use Oxford nanopore technologies and phage display technologies, to obtain quantitative, single-nucleotide resolution maps for any RNA modification of choice. This will allow systematic mapping of RNA modifications for which we currently lack transcriptome-wide maps, as well as investigate the roles, regulation and impact of RNA modifications in proper cellular functioning and cell differentiation. The project will provide significant benefits, such as to the economy by offering a cost-effective alternative to sequencing methods currently used to map DNA and RNA modifications.Read moreRead less
Epigenetic inheritance: how does it work? This project aims to determine the mechanisms responsible for the inheritance of acquired traits. Sometimes the environment can have effects on the phenotype of not only the exposed individual, but also their children and grandchildren. While it is clear that this can occur, what is not clear is the mechanism by which this happens and the frequency at which it happens. This project will use the model organism Caenorhabditis elegans, innovative new techni ....Epigenetic inheritance: how does it work? This project aims to determine the mechanisms responsible for the inheritance of acquired traits. Sometimes the environment can have effects on the phenotype of not only the exposed individual, but also their children and grandchildren. While it is clear that this can occur, what is not clear is the mechanism by which this happens and the frequency at which it happens. This project will use the model organism Caenorhabditis elegans, innovative new techniques for the discovery of low abundance RNA molecules, and fluorescence microscopy to analyse structures within the cell nucleus to determine what the molecular mechanism is by which this "transgenerational epigenetic inheritance" occurs. This will have an impact on evolutionary theory, as well as long-term consequences for the understanding of human health and disease, and the impact of a changing climate on agriculture.Read moreRead less
Challenging current dogma on the inheritance of mitochondrial DNA. Mutations in mitochondrial DNA are often used to infer genetic relationships and have been associated with the expression of human diseases. This project examines the exact mechanism of inheritance of mitochondrial genes to enhance biological interpretations and our understanding of the heritability of specific diseases.
Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cel ....Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cellular processes. The outcomes will include fundamental new knowledge in cell biology and lead to the development of unique biological models that can be used to understand disease.Read moreRead less