Insulin resistance (the inability of ordinarily insulin-sensitive tissues such as muscle and adipose tissue to respond to insulin) contributes to a number of diseases including diabetes and obesity. A key metabolic step in these tissues is the uptake of glucose from the blood stream. This step is accelerated by insulin thus allowing efficient clearance of glucose from the bloodstream after a meal. Our laboratory has played a major role in showing that insulin regulates glucose uptake into muscle ....Insulin resistance (the inability of ordinarily insulin-sensitive tissues such as muscle and adipose tissue to respond to insulin) contributes to a number of diseases including diabetes and obesity. A key metabolic step in these tissues is the uptake of glucose from the blood stream. This step is accelerated by insulin thus allowing efficient clearance of glucose from the bloodstream after a meal. Our laboratory has played a major role in showing that insulin regulates glucose uptake into muscle and adipose tissue by stimulating the movement of a glucose transport protein from inside the cell to the cell surface (see http:--www.imb.uq.edu.au-groups-james-glut4 for an animated description of this process). The purpose of this proposal is to dissect the molecular mechanisms by which this glucose transporter can be held inside the cell in the absence of insulin and then allowed to be released from this site moving to the surface in the presence of insulin. Our studies over the past 5 years have brought us much closer to understanding this process in detail. The identification of the molecules responsible for this regulatory step will not only aid our understanding of this process but it will also provide a valuable target for development of therapeutic agents that can be used to combat insulin resistance.Read moreRead less
Mechanism Of Action Of Sec1p-like Proteins In Membrane Trafficking.
Funder
National Health and Medical Research Council
Funding Amount
$440,250.00
Summary
One of the most important evolutionary changes that has occurred is the development of intracellular compartments. All eukaryotic cells possess numerous membrane-encased structures which provide the basis for intracellular specialisation. For example, in order to degrade unwanted components cells have developed degradative enzymes. It is vital for the cell that these enzymes are sequestered away from other cellular components to avoid destruction of valuable molecules. In addition, the cell has ....One of the most important evolutionary changes that has occurred is the development of intracellular compartments. All eukaryotic cells possess numerous membrane-encased structures which provide the basis for intracellular specialisation. For example, in order to degrade unwanted components cells have developed degradative enzymes. It is vital for the cell that these enzymes are sequestered away from other cellular components to avoid destruction of valuable molecules. In addition, the cell has developed a complex assembly line of modifications that are added to proteins in a specific order as they travel to their final destination within the cell. This necessitates the accurate passage of molecules between compartments, a process known as vesicle transport. To orchestrate the complex network of vesicular transport steps between all of the various intracellular compartments it is necessary to employ complex machinery to guide and check that these steps occur with high fidelity. The goal of our research proposal is to define the function of one of the molecules involved in this control process, the so-called Sec1p proteins. The strength of our proposal lies in the diversity of our approach. We intend to explore the molecular advantages of a relatively simple eukaryotic organism, a yeast cell, and apply the findings obtained from this cell to a more complex but highly related vesicular transport process; that of the insulin-regulated movement of a glucose transporter in mammalian fat and muscle cells. While we intend to apply our findings to the treatment of patients with diabetes, it is our ultimate goal to be able to learn more about this fundamental cell biological process so that we can apply our knowledge to understanding many different disease states.Read moreRead less
The Molecular Mechanism Of Ion-coupled Transport In The Brain
Funder
National Health and Medical Research Council
Funding Amount
$441,407.00
Summary
Cells in the brain communicate through chemical signals called neurotransmitters. Neurotransmitter transporters reside in the membranes of cells and are responsible for regulating levels of these chemicals in the brain. They play an important role in the normal function of the human brain but their dysfunction is responsible for many diseases including Alzheimer's disease and motor neuron disease. It is crucial to understand how these proteins work in both normal and disease states.
Prediction of coronal mass ejections and their radio emissions. This project aims to explain in detail the motion and properties of coronal mass ejections (CMEs) leaving the sun, the radio emissions they generate, and space weather triggers for the sun and other stars. The project expects to create new knowledge in the fields of space, plasma, and astro-physics and space weather research. Expected outcomes include capabilities to accurately predict CMEs and space weather triggers from the sun to ....Prediction of coronal mass ejections and their radio emissions. This project aims to explain in detail the motion and properties of coronal mass ejections (CMEs) leaving the sun, the radio emissions they generate, and space weather triggers for the sun and other stars. The project expects to create new knowledge in the fields of space, plasma, and astro-physics and space weather research. Expected outcomes include capabilities to accurately predict CMEs and space weather triggers from the sun to Earth and theories for type II and IV solar radio bursts. Benefits include high-level training and enhanced human capital and scientific prominence for Australia.Read moreRead less
Integrated Observation, Theory, and Simulation of Type II Solar Radio Bursts. Type II solar radio bursts are associated with solar flares, coronal mass ejections (CMEs), and space weather events at Earth. They are the archetype of collective radio emission associated with shocks. This project aims to: answer longstanding fundamental scientific questions about type IIs using the new Murchison Widefield Array (MWA) and NASA spacecraft, while developing a new Australian capability in solar radio ph ....Integrated Observation, Theory, and Simulation of Type II Solar Radio Bursts. Type II solar radio bursts are associated with solar flares, coronal mass ejections (CMEs), and space weather events at Earth. They are the archetype of collective radio emission associated with shocks. This project aims to: answer longstanding fundamental scientific questions about type IIs using the new Murchison Widefield Array (MWA) and NASA spacecraft, while developing a new Australian capability in solar radio physics; perform new observations and theoretical calculations of relevant emission mechanisms, and, develop a new integrated data-tested theory and simulation capability for type II bursts that explains the emissions quantitatively, and positions us to predict the arrival of CMEs and related space weather at Earth. Read moreRead less
Biomaterials with multifaceted tunability and bio-specificity. Polyurethanes, a family of polymers with independently tunable mechanical and biodegradation properties, will be developed as a versatile platform material for biomedical implants. Novel energetic ion treatments that allow the coupling of bioactive agents to surfaces will eliminate adverse reactions and enable integration with surrounding tissue.
Plasma-catalytic bubbles for sustainable ammonia. Ammonia is one of the world’s most important chemicals directly sustaining over 50% of our food supply. But the current means of its production is highly eco-destructive and responsible for over 1% of global CO2 emissions, a similar value to global air travel. This project aims to produce ammonia from renewable sources of water, electricity and air, which can provide farmers with a zero-carbon fertilizer under a decentralized and even farm-level ....Plasma-catalytic bubbles for sustainable ammonia. Ammonia is one of the world’s most important chemicals directly sustaining over 50% of our food supply. But the current means of its production is highly eco-destructive and responsible for over 1% of global CO2 emissions, a similar value to global air travel. This project aims to produce ammonia from renewable sources of water, electricity and air, which can provide farmers with a zero-carbon fertilizer under a decentralized and even farm-level approach. Moreover, if driven by renewables, ammonia offers an effective means of exporting hydrogen from Australia. Hydrogen has been highlighted by the federal government as a priority technology in its Technology Investment Roadmap with ammonia seen as the best approach for its exportation. Read moreRead less
High productivity of hybrid plasma electrocatalytic fertiliser production. Non-thermal plasma-driven electrocatalytic production of nitrogen fertilisers. The project aims to develop scalable technology for ambient production of fertilisers using renewable energy, air, water, and captured CO2. This project is anticipated to generate new knowledge in plasma catalysis and electrochemical coupling through designing and fine-tuning catalyst-loaded 3D scaffolds. Expected outcomes of this project inclu ....High productivity of hybrid plasma electrocatalytic fertiliser production. Non-thermal plasma-driven electrocatalytic production of nitrogen fertilisers. The project aims to develop scalable technology for ambient production of fertilisers using renewable energy, air, water, and captured CO2. This project is anticipated to generate new knowledge in plasma catalysis and electrochemical coupling through designing and fine-tuning catalyst-loaded 3D scaffolds. Expected outcomes of this project include increasing the capacity to adopt low-cost and decentralised methods for renewable energy utilisation. This should provide substantial technological capacity that can be applied to other sectors of Australia's developing hydrogen economy and expand the use of renewable energy Power-to-X for zero-emissions energy vectors.Read moreRead less
Plasma driven electrochemical synthesis of urea. Urea is the most used nitrogen fertilizer in the world, with more urea manufactured by mass than any other organic chemical. However, the world is experiencing a major shortage of the compound, impacting our food costs and security along with dependent products such as AdBlue (diesel exhaust fluid). Commercial urea production relies on a complex reaction between ammonia and carbon dioxide at high temperatures, which consumes more than 2% of the w ....Plasma driven electrochemical synthesis of urea. Urea is the most used nitrogen fertilizer in the world, with more urea manufactured by mass than any other organic chemical. However, the world is experiencing a major shortage of the compound, impacting our food costs and security along with dependent products such as AdBlue (diesel exhaust fluid). Commercial urea production relies on a complex reaction between ammonia and carbon dioxide at high temperatures, which consumes more than 2% of the world’s energy. This project aims to produce more sustainable urea driven by electricity and using air and captured CO2, through the use of a plasma-driven electrochemical technology, providing farmers with a low-cost fertilizer under a decentralized and secure supply. Read moreRead less