Enhancing Selectivity and Detection in Miniaturised Analytical Separation Systems. Miniaturisation-compatible stationary phases and detection techniques will be developed for microseparation techniques of capillary electrochromatography and chip-based separation systems, and their analytical applications will be investigated. Replaceable stationary phases (RSP) of chromatographic particles suspended in reversible gels will be created by delivering a liquid RSP to the column followed by in situ ....Enhancing Selectivity and Detection in Miniaturised Analytical Separation Systems. Miniaturisation-compatible stationary phases and detection techniques will be developed for microseparation techniques of capillary electrochromatography and chip-based separation systems, and their analytical applications will be investigated. Replaceable stationary phases (RSP) of chromatographic particles suspended in reversible gels will be created by delivering a liquid RSP to the column followed by in situ immobilisation. This approach offers flexibility in optimising a number of parameters of the column and of its use for sample pre-treatment and preconcentration. Pulsed potentiometric detection will be developed for the abovementioned microseparation techniques, combining the advantages of pulsed amperometric techniques with the more universally responding potentiometric detection.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0668471
Funder
Australian Research Council
Funding Amount
$262,706.00
Summary
Hyphenated Capillary Electrophoresis - Mass Spectrometry Facility. The requested funding will facilitate the expansion of the activities of the University of Tasmania (UTas) node of the Australian Centre for Research on Separation Science and its collaborators. This initiative will involve the application of integrated, high resolution technologies for the separation and identification of complex chemical and biological samples. The instrument is to be shared by a number of highly research-activ ....Hyphenated Capillary Electrophoresis - Mass Spectrometry Facility. The requested funding will facilitate the expansion of the activities of the University of Tasmania (UTas) node of the Australian Centre for Research on Separation Science and its collaborators. This initiative will involve the application of integrated, high resolution technologies for the separation and identification of complex chemical and biological samples. The instrument is to be shared by a number of highly research-active groups at UTas in the fields of chemistry, biochemistry, plant and agricultural science, Antarctic studies, and pharmacy where detailed structural identification of components separated from complex mixtures is essential. These projects all focus on fundamental and applied research of great national significance.Read moreRead less
Probing the Tasmanian Devil Serum Proteome for Preclinical Diagnosis of Devil Facial Tumour Disease. The Tasmanian Devil (Sarcophilus harrisii) is a carnivorous marsupial endemic to the island state of Tasmania, and is found all over the state. Over the past decade, a dramatic decline has occurred in the Devil population in association with the emergence of Devil Facial Tumour Disease (DFTD). This project aims to develop a preclinical diagnostic test for DFTD that will provide vital information ....Probing the Tasmanian Devil Serum Proteome for Preclinical Diagnosis of Devil Facial Tumour Disease. The Tasmanian Devil (Sarcophilus harrisii) is a carnivorous marsupial endemic to the island state of Tasmania, and is found all over the state. Over the past decade, a dramatic decline has occurred in the Devil population in association with the emergence of Devil Facial Tumour Disease (DFTD). This project aims to develop a preclinical diagnostic test for DFTD that will provide vital information for use in developing management strategies to ensure the ongoing survival of the Tasmanian Devil. The benefit to Australia in performing this research rests in the new technology that will be developed and the application of this technology to key areas of national and international significance.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100107
Funder
Australian Research Council
Funding Amount
$400,000.00
Summary
Purchase of a multi-purpose Schottky field emission gun scanning electron microscope. Scanning electron microscopy is a basic analytical tool for imaging surfaces of natural and synthetic materials and identification of nanometre-scale features and their compositions. At the University of Tasmania, it supports four of our six designated priority research themes: Antarctic and Marine Studies, Environment, Frontier Technologies, and Sustainable Primary Production. Our research depending on this te ....Purchase of a multi-purpose Schottky field emission gun scanning electron microscope. Scanning electron microscopy is a basic analytical tool for imaging surfaces of natural and synthetic materials and identification of nanometre-scale features and their compositions. At the University of Tasmania, it supports four of our six designated priority research themes: Antarctic and Marine Studies, Environment, Frontier Technologies, and Sustainable Primary Production. Our research depending on this technique includes many fundamental and applied topics from a wide range of disciplines, such as developing portable detection devices for explosives, finding more efficient and sustainable ways to explore for ore, investigating the effects of climate change on marine ecosystems and improving salinity and drought tolerance of crops.Read moreRead less
Towards Microfluidic-Based Advanced Remote Analysis. The research under this project will establish and systematically develop Advanced Remote Analysis as a new inter-disciplinary area and establish a leadership role for Australia. By addressing pressing needs such as monitoring the environment, remote medical diagnostics, advancing Australian science and technology, or monitoring for traces of explosives, this project falls directly into all four of the National Research Priorities with applica ....Towards Microfluidic-Based Advanced Remote Analysis. The research under this project will establish and systematically develop Advanced Remote Analysis as a new inter-disciplinary area and establish a leadership role for Australia. By addressing pressing needs such as monitoring the environment, remote medical diagnostics, advancing Australian science and technology, or monitoring for traces of explosives, this project falls directly into all four of the National Research Priorities with applications addressing corresponding Priority Goals. Other areas benefiting from the outcomes of this project will be remote monitoring of agricultural production including living species, and a number of other industries such as biotechnology, mineral processing, power generation etc.Read moreRead less
Resolving dissolved organic matter: new multi-dimensional separation approaches. To fully understand and model global carbon cycles the source, nature and fate of oceanic dissolved organic carbon is an essential element. This project will develop, model and apply new orthogonol and complementary separation science based technologies to further the comprehensive characterisation and understanding of these complex systems.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0237784
Funder
Australian Research Council
Funding Amount
$707,000.00
Summary
Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-T ....Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-TOFMS technology will validate our newly patented multidimensional separation techniques. Q-TOF-TOFMS technology, novel patented protein prefractionation approaches, and sample handling with high resolution characterisation / identification of new target proteins allows advanced proteomics developments. Proteomics depends critically upon sophisticated MS techniques. These technologies will: ·enhance the capabilities and expertise in these sciences in the SE Australian area; ·ensure the ACROSS initiative achieves internationally competitive research capabilities; ·provide commercial endpoints in fields associated with analysis of proteins, essential oils and other natural (bio)substances.Read moreRead less
Micro-electrofluidic platforms for monitoring 3D human biological models. The ability to study living cells and human biological models (cell cultures) delivers greater understanding of basic biological function and response to applied (bio)chemical stimuli. Creating the physical environments to sustain biological models, and mimic natural conditions and fluidic pathways, is immensely challenging, yet essential to deliver meaningful observational data. This project will deliver this capability t ....Micro-electrofluidic platforms for monitoring 3D human biological models. The ability to study living cells and human biological models (cell cultures) delivers greater understanding of basic biological function and response to applied (bio)chemical stimuli. Creating the physical environments to sustain biological models, and mimic natural conditions and fluidic pathways, is immensely challenging, yet essential to deliver meaningful observational data. This project will deliver this capability through the convergence of expertise and innovation in analytical chemistry, materials science and cellular biology, ultilising the latest technology and understanding of 3D micro/electrofluidics, to enable the study and stimulation of advanced biological models, sustained within precisely controlled 3D micro-environments.Read moreRead less
Lectin based open tubular micro-reactors for probing protein-protein binding. This project seeks to develop non-invasive technology for the measurement and quantitation of lectin-carbohydrate binding events, in the first instance glycopeptides and glycoproteins. The goal is not only to provide accurate protein-protein association and dissociation constant data within the developed system, but to do so within an enclosed micro-fluidic environment, with the added advantages of also providing ‘trap ....Lectin based open tubular micro-reactors for probing protein-protein binding. This project seeks to develop non-invasive technology for the measurement and quantitation of lectin-carbohydrate binding events, in the first instance glycopeptides and glycoproteins. The goal is not only to provide accurate protein-protein association and dissociation constant data within the developed system, but to do so within an enclosed micro-fluidic environment, with the added advantages of also providing ‘trap and release’ extraction capabilities, and being easily coupled to both chromatographic and mass spectrometry systems. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100018
Funder
Australian Research Council
Funding Amount
$630,000.00
Summary
Purchase of a high resolution Nuclear Magnetic Resonance spectrometer with liquid chromatography module. A high resolution Nuclear Magnetic Resonance spectrometer and liquids separation module will support Tasmanian research of international significance across the biological and medical sciences, chemistry and Tasmanian industries including profiling studies in human health, plant biology, molecular basis of disease and complex mixture analysis.