Functional genomics of light stress resistance in the model organism Chlamydomonas: combining molecular genetics, transcriptome and proteome analysis. This project aims at combining molecular genetics, transcriptome and proteome analysis to identify genes and pathways underlying high light stress tolerance in previously isolated mutants of the chlorophyte Chlamydomonas reinhardtii. Comprehensive profiles of transcriptome-proteome linkage will be constructed without the complications of multicel ....Functional genomics of light stress resistance in the model organism Chlamydomonas: combining molecular genetics, transcriptome and proteome analysis. This project aims at combining molecular genetics, transcriptome and proteome analysis to identify genes and pathways underlying high light stress tolerance in previously isolated mutants of the chlorophyte Chlamydomonas reinhardtii. Comprehensive profiles of transcriptome-proteome linkage will be constructed without the complications of multicellularity for this unicellular photosynthetic model organism. We will establish a public proteome reference database and provide new microarrays and molecular markers beneficial for research in Chlamydomonas. We expect to advance understanding of high light resistance mechanisms so that it will eventually be applicable to improve productivity in crop plants growing under various environmental stress conditions.Read moreRead less
Novel photoprotective mechanisms and functional biodiversity of high light tolerance in the model alga Chlamydomonas. Most plants have limited capacity to avoid high light (HL) stress which commonly accompanies drought and high temperature stress. We will identify novel genes and proteins that underlie diverse mechanisms of photoprotection in unique very high light resistant (VHLR) mutants in the alga Chlamydomonas and develop new tools to screen other plants for these attributes. Depending on p ....Novel photoprotective mechanisms and functional biodiversity of high light tolerance in the model alga Chlamydomonas. Most plants have limited capacity to avoid high light (HL) stress which commonly accompanies drought and high temperature stress. We will identify novel genes and proteins that underlie diverse mechanisms of photoprotection in unique very high light resistant (VHLR) mutants in the alga Chlamydomonas and develop new tools to screen other plants for these attributes. Depending on progress, we expect to express them in the higher plant Arabidopsis as a first step towards utilization of VHLR genes for crop improvement. Understanding the mechanisms conferring HL photoprotection is a research priority in plant sciences and will further strengthen Australia's innovative contributions to the internationally networked Chlamydomonas Genome Project.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989589
Funder
Australian Research Council
Funding Amount
$550,000.00
Summary
A massively parallel genome analysis facility for the ACT region. Maintaining a healthy environment, supporting a sustainable agriculture industry and providing excellent healthcare are three key requirements for the future of Australia and Australians. Modern biological research has a major role to play in all three areas. The success and application of this research requires a serious investment in the new technologies that enable a systems-wide high throughput approach to biological questions ....A massively parallel genome analysis facility for the ACT region. Maintaining a healthy environment, supporting a sustainable agriculture industry and providing excellent healthcare are three key requirements for the future of Australia and Australians. Modern biological research has a major role to play in all three areas. The success and application of this research requires a serious investment in the new technologies that enable a systems-wide high throughput approach to biological questions. Co-investing in a massively parallel genome analysis facility to underpin cutting edge environmental, agricultural and biomedical research in the ACT region, will facilitate progress in areas of the utmost importance to the community and future of the nation.Read moreRead less