Plant Protein Signalling Networks. We will assess the functional role of PNPs (novel plant protein hormones) at a biochemical, molecular and cellular level. Importantly, as stresses from climatic extremes are increasing, this will lead to new insights and critical appreciation of the processes plants use to regulate their water status. Since water and solute status underpins the regulation of plant growth and development, these findings will have a major impact on both agriculture and horticul ....Plant Protein Signalling Networks. We will assess the functional role of PNPs (novel plant protein hormones) at a biochemical, molecular and cellular level. Importantly, as stresses from climatic extremes are increasing, this will lead to new insights and critical appreciation of the processes plants use to regulate their water status. Since water and solute status underpins the regulation of plant growth and development, these findings will have a major impact on both agriculture and horticulture in Australia. The new insights that we gain can be used to directly accelerate progress towards the development of plants with improved drought and salinity tolerance that will lead to better crop and pasture productivity under harsh Australian conditions.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0668294
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth anal ....Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth analysis of both nitrogen transport mechanisms and the ability to model root development and water allocation in crop species. This research will ultimately lead to improved knowledge on how plants respond to their environment and where modifications can be made to generate sustainable crops suited to Australian agriculture.Read moreRead less
Mechanistic characterisation of genotype x environment interactions in sorghum and arabidopsis. Sorghum is an economically important cereal crop for Australia. In Australia, sorghum is used as a staple animal feed and it is very important for the live stock industry. With the predicted changes of temperature and rainfall patterns due to climate change, negative effects on sorghum yield are expected, which can have adverse effects on Australian economy. Our studies will identify and mark genes th ....Mechanistic characterisation of genotype x environment interactions in sorghum and arabidopsis. Sorghum is an economically important cereal crop for Australia. In Australia, sorghum is used as a staple animal feed and it is very important for the live stock industry. With the predicted changes of temperature and rainfall patterns due to climate change, negative effects on sorghum yield are expected, which can have adverse effects on Australian economy. Our studies will identify and mark genes that regulate flowering and seed production in sorghum in response to changes in temperature and light interactions. These studies will help to develop novel sorghum varieties with desirable characters through plant-breeding programmes.Read moreRead less
Molecular, physiological and environmental regulation of toxic prussic acid levels (cyanogenesis) in forage sorghum. Forage sorghum is grown widely in dry, tropical areas of Australia. The leaves contain dhurrin, a natural defence product that liberates prussic acid (cyanide) when leaf tissue is disrupted (eg when chewed). The problem is that young plants or those experiencing drought are highly toxic, resulting in financial loss through reduced nutritive value, livestock loss and wasted feed. U ....Molecular, physiological and environmental regulation of toxic prussic acid levels (cyanogenesis) in forage sorghum. Forage sorghum is grown widely in dry, tropical areas of Australia. The leaves contain dhurrin, a natural defence product that liberates prussic acid (cyanide) when leaf tissue is disrupted (eg when chewed). The problem is that young plants or those experiencing drought are highly toxic, resulting in financial loss through reduced nutritive value, livestock loss and wasted feed. Using new, non-GM technology we will identify novel genetically altered sorghum lines with negligible prussic acid. Lines with enhanced levels could be used as soil biofumigants. Breeders can use this germplasm to develop varieties tailored for increasingly dry Australian conditions. The new varieties with controlled dhurrin content will be suitable for export.Read moreRead less
Re-balancing global resources: Manipulating toxic prussic acid (dhurrin) to improve nitrogen use efficiency in forage sorghum in a changing climate. Sorghum is grown widely is Australia and world-wide for forage, grain (mostly for animal feed) and biofuels. It grows well in dry areas. The problem is that the leaves contain a toxin that releases prussic acid (cyanide) that can reduce animal production or even kill stock feeding on it, especially when water stressed. The problem will get worse wit ....Re-balancing global resources: Manipulating toxic prussic acid (dhurrin) to improve nitrogen use efficiency in forage sorghum in a changing climate. Sorghum is grown widely is Australia and world-wide for forage, grain (mostly for animal feed) and biofuels. It grows well in dry areas. The problem is that the leaves contain a toxin that releases prussic acid (cyanide) that can reduce animal production or even kill stock feeding on it, especially when water stressed. The problem will get worse with climate change. Low-cyanide plants developed by us using non-GM methods grow fast, but accumulate nitrate instead which is also toxic. This is a waste of expensive fertiliser too. We aim to develop plants that divert resources to growth instead of toxins in order to reduce fertiliser use and help prepare for the future. The fast growing plants may also be useful as a biofuel crop.Read moreRead less
More than defence: primary roles for cyanogenic glucosides. The tropical crop, sorghum, produces toxic cyanide to avoid being eaten by herbivores, but this diverts resources away from growth and reproduction. Using non-toxic sorghum mutants, this project seeks to explain how cyanide production is regulated and enhance agricultural efficiency in the face of climate change.
Identifying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley. Agrifood production faces the dual challenges of an increasing world population and the threats of abiotic stresses arising from climate change and the erosion of arable land. Cereals, the major food crops, are poorly adapted to tolerate most abiotic stresses, including salinity. This project applies new technologies investigating spatial and temporal biochemical mechanisms a model cereal, Horde ....Identifying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley. Agrifood production faces the dual challenges of an increasing world population and the threats of abiotic stresses arising from climate change and the erosion of arable land. Cereals, the major food crops, are poorly adapted to tolerate most abiotic stresses, including salinity. This project applies new technologies investigating spatial and temporal biochemical mechanisms a model cereal, Hordeum vulgare (barley), utilises to adapt and tolerate salinity. The aims are to investigate the role of specifically plasma membrane lipids modulating either signalling pathways or membrane fluidity that impacts on adaptation during salinity. The results will provide new leads for the development of cereal germplasm with increased salt tolerance.Read moreRead less
Functional analysis of novel mitochondrial outer membrane proteins in Arabidopsis. Mitochondria play central roles in the life and death of cells. This project will characterise the functions of proteins on the mitochondrial surface, which mediate signals that define mitochondrial function, providing novel approaches to modify mitochondrial function and plant growth.
Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for de ....Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for developing crops that are high yielding and also climate resilient. Expected outcomes include full genome sequences for all of Australia’s unique native sorghums, confirmation of new theories on the interrelationships between defence and growth and identification of new traits vital for developing the crops of the future. Read moreRead less
Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We ....Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We will assign
function to each protein and investigate the importance in regulating organelle biogenesis. This will allow us to
modulate plant energy production for optimal growth and to withstand abiotic stress, all of which have
agriculturally beneficial consequences. Read moreRead less