Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, ....Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, cytosolic pH and aluminium uptake across the plasma membrane in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop culti ....Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop cultivars are important part of sustainable farming in Australia. This project will characterise early triggers of Al toxicity in plants, providing a foundation for increasing Al resistance in crop cultivars. Understanding the physiological basis of Al toxicity will lead to improved crop breeding strategies.Read moreRead less
IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the A ....IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the AMT family of ammonium transporters and associated signalling pathways which control the uptake and assimilation of ammonium in plants. This project will confirm the mechanisms involved in ammonium uptake from the soil and lead to the development of ammonium-nitrogen efficient crop plants.Read moreRead less
Plant cell wall - aluminium interactions: a role in aluminium stress. Soil acidity, resulting in Al toxicity, affects production on circa 50% of Australia's intensively used agricultural land, resulting in 8.5 times more foregone agricultural income than dryland salinity (National Land and Water Resources Audit, 2001). Often, remediation with lime is not possible or affordable. By understanding Al reactions with cell wall components, we aim to identify root parameters related to Al resistance by ....Plant cell wall - aluminium interactions: a role in aluminium stress. Soil acidity, resulting in Al toxicity, affects production on circa 50% of Australia's intensively used agricultural land, resulting in 8.5 times more foregone agricultural income than dryland salinity (National Land and Water Resources Audit, 2001). Often, remediation with lime is not possible or affordable. By understanding Al reactions with cell wall components, we aim to identify root parameters related to Al resistance by plants. These parameters will be useful as selection markers to identify and breed Al-tolerant crop genotypes. This, in turn, will improve yields and farm profitability, allowing farmers to use lime to prevent further acidification. This increases sustainability of cropping operations on the 50 Mha with acid soils.Read moreRead less
Functional characterisation of the necrotrophic effector proteins Tox1 and Tox3 from the wheat pathogen Stagonospora nodorum. Fungal pathogens cost the Australian agricultural industry over one billion dollars per year. This project will build upon recent key advances to provide a fundamental basis on how fungal pathogens cause disease. The results from this study will promote future advances in disease management with the aim of securing Australian wheat supplies.
Isolation and functional characterisation of a pathogen meta effector able to inhibit detection of multiple disease effectors by resistant plants. The rust fungi are a major economic threat to crop production in Australia. This project will investigate the molecular mechanism used by a rust fungus to prevent detection of multiple disease-inducing proteins by resistant plants and generate knowledge that will lead to the development of new and more effective disease control strategies.
Molecular basis of rust infection and host plant resistance. Plant diseases threaten agricultural productivity in Australia, with rust fungi being a major problem for cereal grain production. This project will investigate molecular processes underlying the infection of plants by rust fungi and will provide basic knowledge for development of novel and durable disease resistance strategies.
Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plan ....Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plants coordinate and adapt to changing water and salt conditions, addressing a missing link in how ions and water move in and out of plant vacuoles. Benefits include an expanded, innovative range of targets for plant breeding programs to improve plant productivity in our changing climate.Read moreRead less
Stomatal function in transgenic plants with altered guard cell metabolism. Guard cells on the surface of leaves control the rate of water loss and CO2 uptake by changing stomatal aperture in response to environmental signals such light, CO2, humidity and water status. Guard cells therefore play a major role in determining plant productivity and water use efficiency. This project aims to examine the contribution of guard cell energy and carbon metabolism in mediating stomatal responses to the env ....Stomatal function in transgenic plants with altered guard cell metabolism. Guard cells on the surface of leaves control the rate of water loss and CO2 uptake by changing stomatal aperture in response to environmental signals such light, CO2, humidity and water status. Guard cells therefore play a major role in determining plant productivity and water use efficiency. This project aims to examine the contribution of guard cell energy and carbon metabolism in mediating stomatal responses to the environment in intact plants through the generation and analysis of transgenic plants with altered guard cell function. This will aid in the development of strategies for direct manipulation of stomatal function.Read moreRead less
Investigating a new paradigm for plant-pathogen interactions; Identification of host-selective toxin proteins in the wheat pathogen Stagonospora nodorum. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum. Traditional breeding methods have yielded crops that are only mildly resistant leaving control of the disease to be worryingly reliant on fungicides. This project aims to identify and char ....Investigating a new paradigm for plant-pathogen interactions; Identification of host-selective toxin proteins in the wheat pathogen Stagonospora nodorum. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum. Traditional breeding methods have yielded crops that are only mildly resistant leaving control of the disease to be worryingly reliant on fungicides. This project aims to identify and characterise proteins that the fungus secretes to cause disease on wheat. By identifying these proteins, it is anticipated that strategies, both traditional and modern, could be employed to enable better control of the disease. This in turn would help provide a long term and secure supply of wheat and wheat based products to the community.Read moreRead less