Identifying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley. Agrifood production faces the dual challenges of an increasing world population and the threats of abiotic stresses arising from climate change and the erosion of arable land. Cereals, the major food crops, are poorly adapted to tolerate most abiotic stresses, including salinity. This project applies new technologies investigating spatial and temporal biochemical mechanisms a model cereal, Horde ....Identifying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley. Agrifood production faces the dual challenges of an increasing world population and the threats of abiotic stresses arising from climate change and the erosion of arable land. Cereals, the major food crops, are poorly adapted to tolerate most abiotic stresses, including salinity. This project applies new technologies investigating spatial and temporal biochemical mechanisms a model cereal, Hordeum vulgare (barley), utilises to adapt and tolerate salinity. The aims are to investigate the role of specifically plasma membrane lipids modulating either signalling pathways or membrane fluidity that impacts on adaptation during salinity. The results will provide new leads for the development of cereal germplasm with increased salt tolerance.Read moreRead less
Functional analysis of novel mitochondrial outer membrane proteins in Arabidopsis. Mitochondria play central roles in the life and death of cells. This project will characterise the functions of proteins on the mitochondrial surface, which mediate signals that define mitochondrial function, providing novel approaches to modify mitochondrial function and plant growth.
Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for de ....Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for developing crops that are high yielding and also climate resilient. Expected outcomes include full genome sequences for all of Australia’s unique native sorghums, confirmation of new theories on the interrelationships between defence and growth and identification of new traits vital for developing the crops of the future. Read moreRead less
Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We ....Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We will assign
function to each protein and investigate the importance in regulating organelle biogenesis. This will allow us to
modulate plant energy production for optimal growth and to withstand abiotic stress, all of which have
agriculturally beneficial consequences. Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150100825
Funder
Australian Research Council
Funding Amount
$360,000.00
Summary
Characterization of Novel Import/Assembly Pathways in Plant Mitochondria. In addition to their central role in metabolism, plant mitochondria have emerged as important hubs for both sensing and responding to a variety of stimuli. However, as yet there are still many unanswered basic questions about how mitochondria are built in plant cells. This project aims to characterise two novel protein import/assembly pathways, specifically, the newly identified twin-arginine translocation (Tat) protein as ....Characterization of Novel Import/Assembly Pathways in Plant Mitochondria. In addition to their central role in metabolism, plant mitochondria have emerged as important hubs for both sensing and responding to a variety of stimuli. However, as yet there are still many unanswered basic questions about how mitochondria are built in plant cells. This project aims to characterise two novel protein import/assembly pathways, specifically, the newly identified twin-arginine translocation (Tat) protein assembly pathway, and the disulphide relay system of the mitochondrial intermembrane space which displays unique characteristics compared to other systems. A mechanistic understanding of these pathways can be used to design novel strategies to alter plant growth and performance.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210101200
Funder
Australian Research Council
Funding Amount
$453,675.00
Summary
Deciphering how nutrient status impacts plant defence. This project aims to transform our understanding of the relationship between nutrient availability and plant defence. Plant defences are activated by responses to cell wall damage, caused by pathogens. My preliminary data uncovered that the response to cell wall damage depends on the nitrogen status of the plant; providing a direct link between nutrients and defence. The research will use new mutants that disengage this link to uncover molec ....Deciphering how nutrient status impacts plant defence. This project aims to transform our understanding of the relationship between nutrient availability and plant defence. Plant defences are activated by responses to cell wall damage, caused by pathogens. My preliminary data uncovered that the response to cell wall damage depends on the nitrogen status of the plant; providing a direct link between nutrients and defence. The research will use new mutants that disengage this link to uncover molecular mechanisms underlying this process. The outcomes will provide new approaches to breed crop plants with improved nitrogen use efficiency and disease resistance. It will benefit agriculture by reducing the use of costly fertilisers and pesticides and mitigate the huge environmental damage they cause.Read moreRead less
The role of the ammonium transport bHLHm1/AMF1 regulatory loci in plants. This project aims to investigate the role of a regulatory locus in the regulation of ammonium transport in plants and the interacting genetic and biochemical signalling promoting the interaction. Ammonium is an important nutrient source for plant growth and development. It has been recently identified that a new transport mechanism (AMF1 ) mediates ammonium transport across legume root nodule cellular membranes. AMF1 was i ....The role of the ammonium transport bHLHm1/AMF1 regulatory loci in plants. This project aims to investigate the role of a regulatory locus in the regulation of ammonium transport in plants and the interacting genetic and biochemical signalling promoting the interaction. Ammonium is an important nutrient source for plant growth and development. It has been recently identified that a new transport mechanism (AMF1 ) mediates ammonium transport across legume root nodule cellular membranes. AMF1 was identified through a transcriptional interaction with a membrane localised bHLHm1 transcription factor. Both bHLHm1 and AMF1 belong to a unique chromosomal regulatory locus common across sequenced dicot plant species.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE130100320
Funder
Australian Research Council
Funding Amount
$374,000.00
Summary
Proteomic analysis of thermal response in plants. This project will identify macromolecules that regulate temperature response in plants. Understanding how plants perceive changes in temperature will allow crop improvement in the face of likely increasing temperatures.
Molecular and cellular mechanisms of action of novel plant guanylyl cyclase enzymes - a new class of overlapping dual-domain molecules. A group of highly unusual catalytic molecules in plants has been identified. The mechanisms of action of these molecules will be studied in this project to learn their role in regulating plant growth in changing climates. The results will reveal how these molecules function and also provide new insights for the development of multi-functional artificial molecule ....Molecular and cellular mechanisms of action of novel plant guanylyl cyclase enzymes - a new class of overlapping dual-domain molecules. A group of highly unusual catalytic molecules in plants has been identified. The mechanisms of action of these molecules will be studied in this project to learn their role in regulating plant growth in changing climates. The results will reveal how these molecules function and also provide new insights for the development of multi-functional artificial molecules.Read moreRead less
Re-balancing global resources: Manipulating toxic prussic acid (dhurrin) to improve nitrogen use efficiency in forage sorghum in a changing climate. Sorghum is grown widely is Australia and world-wide for forage, grain (mostly for animal feed) and biofuels. It grows well in dry areas. The problem is that the leaves contain a toxin that releases prussic acid (cyanide) that can reduce animal production or even kill stock feeding on it, especially when water stressed. The problem will get worse wit ....Re-balancing global resources: Manipulating toxic prussic acid (dhurrin) to improve nitrogen use efficiency in forage sorghum in a changing climate. Sorghum is grown widely is Australia and world-wide for forage, grain (mostly for animal feed) and biofuels. It grows well in dry areas. The problem is that the leaves contain a toxin that releases prussic acid (cyanide) that can reduce animal production or even kill stock feeding on it, especially when water stressed. The problem will get worse with climate change. Low-cyanide plants developed by us using non-GM methods grow fast, but accumulate nitrate instead which is also toxic. This is a waste of expensive fertiliser too. We aim to develop plants that divert resources to growth instead of toxins in order to reduce fertiliser use and help prepare for the future. The fast growing plants may also be useful as a biofuel crop.Read moreRead less