Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561161
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our p ....Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our present capacity for Xenopus oocyte expression analysis of nutrient transport proteins. This facility will greatly aid our current research quantum in this field and allow for new discoveries related to nutrient transport in plants.Read moreRead less
The role of the ammonium transport bHLHm1/AMF1 regulatory loci in plants. This project aims to investigate the role of a regulatory locus in the regulation of ammonium transport in plants and the interacting genetic and biochemical signalling promoting the interaction. Ammonium is an important nutrient source for plant growth and development. It has been recently identified that a new transport mechanism (AMF1 ) mediates ammonium transport across legume root nodule cellular membranes. AMF1 was i ....The role of the ammonium transport bHLHm1/AMF1 regulatory loci in plants. This project aims to investigate the role of a regulatory locus in the regulation of ammonium transport in plants and the interacting genetic and biochemical signalling promoting the interaction. Ammonium is an important nutrient source for plant growth and development. It has been recently identified that a new transport mechanism (AMF1 ) mediates ammonium transport across legume root nodule cellular membranes. AMF1 was identified through a transcriptional interaction with a membrane localised bHLHm1 transcription factor. Both bHLHm1 and AMF1 belong to a unique chromosomal regulatory locus common across sequenced dicot plant species.Read moreRead less
Integrative behaviour: a new synthesis. The research will use a uniquely integrative behavioural approach to a) analyse swarming in locusts, and b) apply powerful new models in nutrition to address key problems in biology, agriculture and human health. Outcomes will include i) an understanding of the molecular and environmental mechanisms controlling swarming in locusts, providing new management and control strategies; ii) insights into human obesity, with health policy recommendations; iii) new ....Integrative behaviour: a new synthesis. The research will use a uniquely integrative behavioural approach to a) analyse swarming in locusts, and b) apply powerful new models in nutrition to address key problems in biology, agriculture and human health. Outcomes will include i) an understanding of the molecular and environmental mechanisms controlling swarming in locusts, providing new management and control strategies; ii) insights into human obesity, with health policy recommendations; iii) new technologies for designing nutritional regimes for animal production; iv) an understanding of the responses of insect pests to changing environments; and v) insights into the flow of nutrients across trophic levels within terrestrial ecosystems.Read moreRead less
Cover crops that decrease phosphorus (P) transport from agricultural soils by increasing the efficiency of P fertiliser application. The aim of this project is to develop cover crop practices for the heavily fertilised potato cropping soils of the Robertson district that will minimise erosion and runoff of water potentially rich in phosphorus (P), thereby resulting in significant improvements in the environmental management of this sensitive catchment area. The cover crops will: (i) improve infi ....Cover crops that decrease phosphorus (P) transport from agricultural soils by increasing the efficiency of P fertiliser application. The aim of this project is to develop cover crop practices for the heavily fertilised potato cropping soils of the Robertson district that will minimise erosion and runoff of water potentially rich in phosphorus (P), thereby resulting in significant improvements in the environmental management of this sensitive catchment area. The cover crops will: (i) improve infiltration, decrease erosion, and off-site transport of P; (ii)increase access to accumulated soil P, making it available to a subsequent potato crop, thus decreasing the P loading of the soil; and (iii) have a biofumigation effect that will reduce the use of soil fumigants.
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High temperature limits of leaf function. In arid and semi-arid central Australia, Acacia spp. dominate the over-storey, but this shifts to Eucalyptus and Corymbia spp. in more mesic coastal regions. Areas of central Australia are extremely hot, dry and sunny, and it is this combination of stresses that likely excludes Eucalyptus spp. from many landforms. There has been little research on high temperature tolerance of Acacia and Eucalyptus, despite the putative importance of this stress, in co ....High temperature limits of leaf function. In arid and semi-arid central Australia, Acacia spp. dominate the over-storey, but this shifts to Eucalyptus and Corymbia spp. in more mesic coastal regions. Areas of central Australia are extremely hot, dry and sunny, and it is this combination of stresses that likely excludes Eucalyptus spp. from many landforms. There has been little research on high temperature tolerance of Acacia and Eucalyptus, despite the putative importance of this stress, in combination with other stresses, in limiting species? distributions. Our program of collaborative research will examine the tolerance of Acacia and Eucalyptus to a combination of high temperatures, drought and high light.Read moreRead less
Symbiotic transport proteins in legumes. Some plants form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins wh ....Symbiotic transport proteins in legumes. Some plants form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins which govern nutrient exchange between the partners and development of the special structures in the roots that house the bacteria. Subsequent manipulation of these genes and proteins may allow us to identify control points and enhance nitrogen fixation.
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Molecular analysis of the symbiotic interface of nitrogen-fixing legumes. Some legumes form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on ....Molecular analysis of the symbiotic interface of nitrogen-fixing legumes. Some legumes form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins which govern nutrient exchange between the partners and development of the special structures in the roots that house the bacteria. Subsequent manipulation of these genes and proteins may allow us to identify control points and enhance nitrogen fixation.Read moreRead less
Understanding plant uptake of organic and inorganic nitrogen for optimal fertiliser application in forestry. Nitrogen (N) in soils occurs in both organic and inorganic forms. Plants can take up inorganic N - nitrate and ammonium - but, on average, these account for only 5% of the soluble N in soils. Recent evidence suggests that plants may be able to tap into some of the 95% of N that occurs in organic forms. We will investigate the importance of organic N uptake for two plantation Eucalyptus sp ....Understanding plant uptake of organic and inorganic nitrogen for optimal fertiliser application in forestry. Nitrogen (N) in soils occurs in both organic and inorganic forms. Plants can take up inorganic N - nitrate and ammonium - but, on average, these account for only 5% of the soluble N in soils. Recent evidence suggests that plants may be able to tap into some of the 95% of N that occurs in organic forms. We will investigate the importance of organic N uptake for two plantation Eucalyptus species by tracing the uptake of different N forms by bacteria, fungi and eucalypts. This information will redefine what is meant by 'available N' and will guide the development of a new test for soil N status.Read moreRead less
Coal-ash as a resource for sustainable soil-management in plant production systems. Acidification and salinisation are major causes of land degradation in Australia, costing $1-2 billion to combat. Farmers used 1.9M t of lime and 1.0M t of gypsum to manage their soils in 2000. Power stations annually produce 12M t of coal-ash that have functional characteristics of lime and gypsum and can potentially ameliorate degraded land. Presently only 43% of the ash is used and almost entirely for constr ....Coal-ash as a resource for sustainable soil-management in plant production systems. Acidification and salinisation are major causes of land degradation in Australia, costing $1-2 billion to combat. Farmers used 1.9M t of lime and 1.0M t of gypsum to manage their soils in 2000. Power stations annually produce 12M t of coal-ash that have functional characteristics of lime and gypsum and can potentially ameliorate degraded land. Presently only 43% of the ash is used and almost entirely for construction with the rest going into landfills. This project will determine the sustainable use of coal-ash to improve structure and nutritive properties of the soil and to raise crop yield across several sites in Australia.Read moreRead less
Exploring the genetic and functional diversity nexus in ericoid mycorrhizal and related symbioses. Epacrids are important components of the Australian flora and several are considered threatened, yet we know relatively little regarding the importance of ericoid mycorrhizal fungal diversity to their survival. The proposed work will provide essential information on the functional significance of ericoid mycorrhizal endophyte diversity in the growth and survival of epacrids. It will further lead to ....Exploring the genetic and functional diversity nexus in ericoid mycorrhizal and related symbioses. Epacrids are important components of the Australian flora and several are considered threatened, yet we know relatively little regarding the importance of ericoid mycorrhizal fungal diversity to their survival. The proposed work will provide essential information on the functional significance of ericoid mycorrhizal endophyte diversity in the growth and survival of epacrids. It will further lead to improved propagation of epacrids and better informed decisions for sustainable management of Australian native vegetation.Read moreRead less