"Painting" the 3D proteome: folding, conformation and interactions. The project aims to develop a "residue painting approach", employing novel chemical biology reagents and advanced quantitative proteomics, to monitor changes in protein folding, conformations and interactions in cells, in response to stimuli. Proteins direct almost all functions required to sustain life. The project expects to map the dynamic 3D-structures of thousands of proteins that inform the networks they are in, and of the ...."Painting" the 3D proteome: folding, conformation and interactions. The project aims to develop a "residue painting approach", employing novel chemical biology reagents and advanced quantitative proteomics, to monitor changes in protein folding, conformations and interactions in cells, in response to stimuli. Proteins direct almost all functions required to sustain life. The project expects to map the dynamic 3D-structures of thousands of proteins that inform the networks they are in, and of the conformations they adopt. Expected outcomes include the development of novel biotechnology tools for protein structure and function analysis, the illumination of important cell biology pathways underpinning molecular responses to stimuli and stress, and the training of our next generation of scientists.Read moreRead less
Making muscle: molecular dissection of membrane domain formation. For a muscle to contract efficiently in response to an electrical signal it requires the formation of an extensive system of hollow membranous tubules through which the signal can be propagated. This proposal addresses the molecular mechanisms involved in the formation of this tubule system in skeletal muscle. This project will develop cell biology in a whole organism rather than a cell culture system and provide a new framework f ....Making muscle: molecular dissection of membrane domain formation. For a muscle to contract efficiently in response to an electrical signal it requires the formation of an extensive system of hollow membranous tubules through which the signal can be propagated. This proposal addresses the molecular mechanisms involved in the formation of this tubule system in skeletal muscle. This project will develop cell biology in a whole organism rather than a cell culture system and provide a new framework for Australian and international cell biologists. It will generate new knowledge, train young Australian scientists, help build international collaborative networks and engage the public outside the research community.Read moreRead less
The core inflammasome as a model for caspase activation. This project aims to change the paradigm for the structure of the active inflammasome. Inflammasomes activate caspases, enzymes central to cell death and inflammatory processes. The current concept of inflammasomes is that caspases are recruited into a single massive protein complex seen as a “speck” in the cell. This project proposes the speck is a terminal stage, after the major enzymatic activity is over. This project aims to purify sma ....The core inflammasome as a model for caspase activation. This project aims to change the paradigm for the structure of the active inflammasome. Inflammasomes activate caspases, enzymes central to cell death and inflammatory processes. The current concept of inflammasomes is that caspases are recruited into a single massive protein complex seen as a “speck” in the cell. This project proposes the speck is a terminal stage, after the major enzymatic activity is over. This project aims to purify smaller early stage inflammasome complexes, for structural analysis. The outcome will be a clearer understanding of processes of caspase activation and inflammasome formation. This will provide significant benefits, such as improve our understanding of processes of cell death and innate immunity, and train students.Read moreRead less
Applications-oriented elucidation of germination triggers for Emu Bush seed. The project aims to determine the environmental and genetic mechanisms that currently limit seed germination in Emu Bush (Eremophila) species. The anticipated project outcomes aim to develop new technologies for efficient and mass production of Emu Bush seedlings. The outcomes will improve land restoration by increasing plant diversity and reducing establishment costs, and will also provide the nursery industry with nov ....Applications-oriented elucidation of germination triggers for Emu Bush seed. The project aims to determine the environmental and genetic mechanisms that currently limit seed germination in Emu Bush (Eremophila) species. The anticipated project outcomes aim to develop new technologies for efficient and mass production of Emu Bush seedlings. The outcomes will improve land restoration by increasing plant diversity and reducing establishment costs, and will also provide the nursery industry with novel products for home gardens. The intended project benefits are to increase the diversity of Australian native plants used for restoration and ornamental purposes and to promote the conservation of species in this plant family and its genetic diversity.Read moreRead less
An integrated nano-bioengineered chip for enhanced molecular evolution. This project aims to develop a novel molecular evolution platform technology for the rapid selection of high value target binding molecules from diverse molecular libraries using an electrically activated nanofluidic chip coated with target. Significant outcomes from the project is the controlled selection of target binding molecules that is not possible with current methods and improved understanding of nanoforce driven mol ....An integrated nano-bioengineered chip for enhanced molecular evolution. This project aims to develop a novel molecular evolution platform technology for the rapid selection of high value target binding molecules from diverse molecular libraries using an electrically activated nanofluidic chip coated with target. Significant outcomes from the project is the controlled selection of target binding molecules that is not possible with current methods and improved understanding of nanoforce driven molecular collisions on nano-bioengineered surfaces. This provides significant benefits, creating new knowledge in nanomaterials and advanced manufacturing of nanofabricated devices, creating commercial interest and positioning Australia at the forefront of molecular discovery technology, a highly valuable global market.
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Intelligent bioprocessing for next-generation nutritional yeast extracts. This project aims to provide new, science-based levers for optimising the industrial production of tailormade yeast extracts for food applications. Advanced biochemical and engineering methods will be used to develop new knowledge of the links between yeast growth conditions, cell biochemistry, processing and the flavour and texture profiles of yeast hydrolysates. This understanding will allow the properties of yeast hydro ....Intelligent bioprocessing for next-generation nutritional yeast extracts. This project aims to provide new, science-based levers for optimising the industrial production of tailormade yeast extracts for food applications. Advanced biochemical and engineering methods will be used to develop new knowledge of the links between yeast growth conditions, cell biochemistry, processing and the flavour and texture profiles of yeast hydrolysates. This understanding will allow the properties of yeast hydrolysates to be accurately tuned during yeast production and processing. The resulting process improvements and innovations will increase the efficiency and quality of current yeast extract products and allow the development of new food products.Read moreRead less
Molecular networks underlying mitochondrial biogenesis in humans. Mitochondria are essential for life, and we propose a highly-innovative approach (employing multiple, cutting-edge ‘omic’ technologies and bioinformatics) to advance the fundamental understanding of how mitochondria respond and adapt to exercise in humans. The project outcomes should include significant new knowledge and advanced expertise that can be used by others to facilitate additional research outcomes. The project anticipa ....Molecular networks underlying mitochondrial biogenesis in humans. Mitochondria are essential for life, and we propose a highly-innovative approach (employing multiple, cutting-edge ‘omic’ technologies and bioinformatics) to advance the fundamental understanding of how mitochondria respond and adapt to exercise in humans. The project outcomes should include significant new knowledge and advanced expertise that can be used by others to facilitate additional research outcomes. The project anticipates the contribution of innovative tools for molecular biology research, benefiting therapeutic and biotechnology applications. This project will support advanced training of young researchers in frontier technologies, which will expand Australian research capabilities and help produce a higher quality workforce.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE240100134
Funder
Australian Research Council
Funding Amount
$796,206.00
Summary
Super-resolution platform to accelerate biological and molecular research. This application aims to establish a new molecular analysis platform integrating a microfluid capillary electrophoresis interface directly to a mass spectrometer with advanced data scanning technology. This enables label-free detection, quantitation and characterisation of intact proteins, lipids and metabolites with unprecedented sensitivity, resolution and throughput. It will enhance ARC projects spanning natural produc ....Super-resolution platform to accelerate biological and molecular research. This application aims to establish a new molecular analysis platform integrating a microfluid capillary electrophoresis interface directly to a mass spectrometer with advanced data scanning technology. This enables label-free detection, quantitation and characterisation of intact proteins, lipids and metabolites with unprecedented sensitivity, resolution and throughput. It will enhance ARC projects spanning natural product discovery, biotechnology, agriculture, and animal, plant and marine biology, as well as single-cell proteomics, lipidomics and metabolomics. It will ensure Australia remains at the forefront of molecular and biological research and create new training and collaborative opportunities both nationally and internationally.Read moreRead less
The role of protein glycosylation in erythropoiesis . This project aims to understand how the sugar code of key-signalling proteins influences the development of red blood cells. This project expects to generate new fundamental knowledge in the area of stem cell signalling by innovative integration of biological and computational molecular characterisation techniques. The expected outcomes of this project include the development of novel workflows to study key regulators of cell development and ....The role of protein glycosylation in erythropoiesis . This project aims to understand how the sugar code of key-signalling proteins influences the development of red blood cells. This project expects to generate new fundamental knowledge in the area of stem cell signalling by innovative integration of biological and computational molecular characterisation techniques. The expected outcomes of this project include the development of novel workflows to study key regulators of cell development and the generation of new knowledge in stem cell signalling that will find applications in transforming stem cell therapies and associated research for future applications such as the laboratory manufacturing of red blood cells to close the availability gap for transfusion purposes.Read moreRead less
Cellular Ageing: Is the Plasma Membrane the Control Hub? This project aims to determine whether the plasma membrane lipid composition is a major driver of cellular ageing. It expects to generate new knowledge in the molecular mechanism of cellular ageing, utilising our team’s deep expertise in lipid biology, bioinformatics, biophysics, extracellular vesicle biology and cellular ageing. Expected outcomes include the identification of novel cellular ageing markers and anti-ageing targets while als ....Cellular Ageing: Is the Plasma Membrane the Control Hub? This project aims to determine whether the plasma membrane lipid composition is a major driver of cellular ageing. It expects to generate new knowledge in the molecular mechanism of cellular ageing, utilising our team’s deep expertise in lipid biology, bioinformatics, biophysics, extracellular vesicle biology and cellular ageing. Expected outcomes include the identification of novel cellular ageing markers and anti-ageing targets while also cementing long-standing partnerships and fostering new interdisciplinary collaborations. This cellular ageing study will provide novel insights into the basic principles of cellular behaviour, e.g. growth, differentiation, communication and death, reinforcing Australia’s leadership in biological science.Read moreRead less