Single cell glycomics: mapping the surface glycan signature of cells. This project aims to develop a platform technology for multiplexed glycan mapping of the surface of a single cell to address challenges of functional glycomics by utilising a conceptually new approach. By combining newly designed plasmonic nanoparticles with surface-enhanced Raman scattering tags and multiple specific carbohydrate-recognising lectins, this project expects to produce a generic technology that is capable of non- ....Single cell glycomics: mapping the surface glycan signature of cells. This project aims to develop a platform technology for multiplexed glycan mapping of the surface of a single cell to address challenges of functional glycomics by utilising a conceptually new approach. By combining newly designed plasmonic nanoparticles with surface-enhanced Raman scattering tags and multiple specific carbohydrate-recognising lectins, this project expects to produce a generic technology that is capable of non-destructive barcoding of the surface glycan signature of single cells in their native state and in response to metabolic perturbations. Expected project outcomes include advancing knowledge in nanobiotechnology, glycobiology and cell biology by being able to easily monitor changes to the surface of single cells.Read moreRead less
The combined use of proteomics and small molecules for target identification and pathway analysis. This project intends to investigate how a series of new small molecules identified from our research to improve the metabolic effects of insulin. This project will integrate medicinal chemistry with proteomics and metabolic biology to identify the cellular targets and their mechanism of action.
Discovery Early Career Researcher Award - Grant ID: DE120102556
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
The influence of crosstalk between protein post-translational modifications on the propagation of molecular signals. The ability of a cell to respond appropriately to its surroundings is a result of interactions between proteins and chemical modifiers termed post-translational modifications (PTM). This project will show how PTM interactions (competition/ cooperation) influence cellular outcomes in response to changes in the environment.