How evolution is constrained by trade-offs between the multiplication and survival of organisms. The negative correlation between reproduction (production of large numbers of progeny) and survival (resistance to external challenges) is a crucial trade-off that limits the evolution of perfect organisms. Such trade-offs are extremely difficult to study in closely controlled experiments because of the complexities in biological organisation and life-cycles. This project will explore trade-offs usin ....How evolution is constrained by trade-offs between the multiplication and survival of organisms. The negative correlation between reproduction (production of large numbers of progeny) and survival (resistance to external challenges) is a crucial trade-off that limits the evolution of perfect organisms. Such trade-offs are extremely difficult to study in closely controlled experiments because of the complexities in biological organisation and life-cycles. This project will explore trade-offs using a novel synthetic biology strategy. Genes in bacteria will be engineered to produce strains with a range of fixed but different trade-off settings. The strain sets will allow unprecedented analysis of reproduction-survival trade-offs and testing of important models of how trade-offs control fitness and evolutionary outcomes.Read moreRead less
The biology of integrons and their role in bacterial adaptation. Bacteria evolve in ways that animals and plants do not. One of the tools available is the ability to share genes amongst individuals in a community. One example of this is the very rapid spread of antibiotic resistance genes in pathogens. Here we will be studying a genetic element that greatly contributes to this horizontal spread of genes. This will lead to a better understanding of how bacteria work, the direct benefits of whic ....The biology of integrons and their role in bacterial adaptation. Bacteria evolve in ways that animals and plants do not. One of the tools available is the ability to share genes amongst individuals in a community. One example of this is the very rapid spread of antibiotic resistance genes in pathogens. Here we will be studying a genetic element that greatly contributes to this horizontal spread of genes. This will lead to a better understanding of how bacteria work, the direct benefits of which includes the discovery of new pathways and genes for the biotechnology industry and greater understanding of how bacteria cause disease in us, other animals and in commercial crops.Read moreRead less
A functional genomic approach for understanding metal ion adaptation in marine cyanobacteria. Unicellular marine cyanobacteria constitute 20-40% of total marine chlorophyll biomass and carbon fixation, and hence significantly impact the global carbon cycle and are very relevant to combating global warming. This research will reveal some of the major mechanisms by which marine cyanobacteria have adapted to metal levels in coastal and oligotrophic environments. Thus these results will help us und ....A functional genomic approach for understanding metal ion adaptation in marine cyanobacteria. Unicellular marine cyanobacteria constitute 20-40% of total marine chlorophyll biomass and carbon fixation, and hence significantly impact the global carbon cycle and are very relevant to combating global warming. This research will reveal some of the major mechanisms by which marine cyanobacteria have adapted to metal levels in coastal and oligotrophic environments. Thus these results will help us understand the distribution and diversity of these organisms in relation to global primary productivity. They will also lead to the development of more robust biomarkers for metal stress and pollution in coastal environments.Read moreRead less
The only constant is change: ecology and evolution of phage-host interactions in a model ecosystem. Microorganisms underpin life on Earth, but our understanding of their diversity and activity is limited by our inability to grow most of them in the laboratory. Recently, new techniques have emerged that allow access to the genetic information of all microorganisms by directly sequencing DNA and RNA from the environment. In this research we will further develop these frontier technologies, promoti ....The only constant is change: ecology and evolution of phage-host interactions in a model ecosystem. Microorganisms underpin life on Earth, but our understanding of their diversity and activity is limited by our inability to grow most of them in the laboratory. Recently, new techniques have emerged that allow access to the genetic information of all microorganisms by directly sequencing DNA and RNA from the environment. In this research we will further develop these frontier technologies, promoting this new area of science in Australia. We will apply these techniques to microbial communities involved in wastewater treatment in order to understand the interactions between microorganisms and the viruses that infect them. Understanding this interaction will have important implications for optimising these treatment processes.Read moreRead less
The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples. Macquarie University and the University of Copenhagen have expertise in fluorescence detection and whole cell biosensors respectively. The project will take advantage of these skills and develop a sensitive assay for monitoring biosensor bacteria in soil. The technology will be significant as it will enable real time analysis of antibiotic production in situ through the de ....The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples. Macquarie University and the University of Copenhagen have expertise in fluorescence detection and whole cell biosensors respectively. The project will take advantage of these skills and develop a sensitive assay for monitoring biosensor bacteria in soil. The technology will be significant as it will enable real time analysis of antibiotic production in situ through the detection of GFP expression. This work will then be used to isolate new antibiotic produces and will be extended to research into the bioavailability of toxic compounds and stress. An existing collaboration between the two institutions will be extended enabling the transfer and application of biosensor technology to Australia.Read moreRead less
Genome-level insight into the dynamics of a model coral microbiome. The aim of the project is to examine structural and functional microbiome dynamics in an ecologically important coral on the Great Barrier Reef along a natural temperature gradient. Microorganisms form an intimate symbiotic relationship with corals and are critical to their health. However, the microbiome can be disrupted by environmental perturbations, including higher-than-normal ocean temperatures, leaving the coral susceptib ....Genome-level insight into the dynamics of a model coral microbiome. The aim of the project is to examine structural and functional microbiome dynamics in an ecologically important coral on the Great Barrier Reef along a natural temperature gradient. Microorganisms form an intimate symbiotic relationship with corals and are critical to their health. However, the microbiome can be disrupted by environmental perturbations, including higher-than-normal ocean temperatures, leaving the coral susceptible to disease and bleaching. Currently, our understanding of how the microbiome composition and metabolic function change in response to seasonal temperature variation and disease is limited. This project is designed to provide insight into the role the microbiome plays in maintaining coral health and may aid in the long-term preservation of the reefs.Read moreRead less
Defining how bacteriophage shape the biofilm lifecycle of bacteria. Bacteriophages are viruses that infect bacteria and they represent a significant selective pressure that drives the evolution of bacteria. We will study the genetic mechanisms by which genes encoded by a bacteriophage can contribute to increased survival of bacteria in the environment.
Environmental metagenomics, metaproteomics and novel bioactives from microbial communities in Antarctic lakes. This program will derive an integrated understanding of microbial ecology which is essential for determining ways of preserving the health of the World's ecosystems. Through this, Australia will remain a world leader in Antarctic biology, strengthening Australia's reputation in technologically innovative scientific programs of global significance, training local scientists in cutting ed ....Environmental metagenomics, metaproteomics and novel bioactives from microbial communities in Antarctic lakes. This program will derive an integrated understanding of microbial ecology which is essential for determining ways of preserving the health of the World's ecosystems. Through this, Australia will remain a world leader in Antarctic biology, strengthening Australia's reputation in technologically innovative scientific programs of global significance, training local scientists in cutting edge genomic biology and fostering the interests of the international community in sciences ranging from microbial ecology to bioprospecting. Novel biodegradable enzymes will be developed to replace harsh chemicals providing environmentally friendly, cheaper and more effective agents for use in medical, biotechnological, industrial and biodefense applications.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE180100046
Funder
Australian Research Council
Funding Amount
$365,058.00
Summary
Capturing highly beneficial and persistent legume symbionts. This project aims to investigate impacts of climate change on the survival of symbiotic soil bacteria and the nutritional benefits they provide plants, using molecular technology. This project will generate new knowledge about the process of adaptation in symbiotic bacteria, by measuring genomic changes. Expected outcomes of this project include enhanced capacity to design ecological or genetic manipulations of soil bacteria to augment ....Capturing highly beneficial and persistent legume symbionts. This project aims to investigate impacts of climate change on the survival of symbiotic soil bacteria and the nutritional benefits they provide plants, using molecular technology. This project will generate new knowledge about the process of adaptation in symbiotic bacteria, by measuring genomic changes. Expected outcomes of this project include enhanced capacity to design ecological or genetic manipulations of soil bacteria to augment plant survival and health. Anticipated benefits include enhanced woodland restoration in a biodiversity hotspot, options to mitigate habitat damage from climate change, and strategies to increase agricultural productivity with less fertiliser.Read moreRead less
Evolution of diverse symbiotic phenotypes among native soil bacteria following spread of a genomic island from a rhizobial inoculant. The quality of legume protein depends on symbiotic nitrogen fixation by root nodule bacteria (RNB). Sustainable legume production in Australian agriculture depends on legume inoculation with effective strains of these bacteria. Unfortunately inoculant strains transfer DNA to other soil bacteria resulting in soil populations of RNB that compete for nodulation but a ....Evolution of diverse symbiotic phenotypes among native soil bacteria following spread of a genomic island from a rhizobial inoculant. The quality of legume protein depends on symbiotic nitrogen fixation by root nodule bacteria (RNB). Sustainable legume production in Australian agriculture depends on legume inoculation with effective strains of these bacteria. Unfortunately inoculant strains transfer DNA to other soil bacteria resulting in soil populations of RNB that compete for nodulation but are less effective in nitrogen fixation. This transfer of DNA threatens a $2 billion asset in Australian agriculture. We will use molecular microbial ecology to investigate the mechanisms of genetic transfer of symbiotic DNA in RNB, and use this knowledge to prevent it.Read moreRead less