Discovery Early Career Researcher Award - Grant ID: DE120101706
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Worth its salt: advancing knowledge of salinity tolerance with mangroves as a model system. This project aims to understand how salinity tolerance is achieved in mangroves, which are highly salt tolerant plants. Using a combination of physiological and gene expression technologies the project will measure the response of mangroves to elevated salinity and atmospheric carbon dioxide, contributing to the development of salt tolerant next generation crops.
Regulation of ion homeostasis by two-component signalling elements. Dryland salinity is continuously claiming Australian lands and is a serious threat to our agricultural industry, native flora and fauna, and infrastructure. Attempting to feed an increasing population whilst agricultural land is disappearing, places an ever-increasing burden on our remaining land. While there is no simple solution to this problem, understanding how plants regulate cellular ion concentrations will help to generat ....Regulation of ion homeostasis by two-component signalling elements. Dryland salinity is continuously claiming Australian lands and is a serious threat to our agricultural industry, native flora and fauna, and infrastructure. Attempting to feed an increasing population whilst agricultural land is disappearing, places an ever-increasing burden on our remaining land. While there is no simple solution to this problem, understanding how plants regulate cellular ion concentrations will help to generate plants that are suitable for restoration of damaged lands or crop plants that are more tolerant to saline soils. Furthermore, knowledge acquired from the proposed project will also be useful for generating healthier crop plants with enhanced levels of ions essential for the human diet. Read moreRead less
The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will st ....The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will study for the first time the role of the beta-gamma subunit in plants. We will use a comprehensive approach combining biochemical, physiological, phenotypic, genomic and proteomic studies.Read moreRead less
Top-down rehydration: role of multiple water sources in mangrove function. This project aims to combine cutting-edge analytical and imaging techniques to assess contributions of atmospheric water sources to shoot-water balances, identify leaf traits associated with top-down rehydration, and determine the relative importance of different sources of water used by mangroves in maintenance of photosynthetic carbon assimilation along natural gradients in salinity and aridity. The capacity of shoots ....Top-down rehydration: role of multiple water sources in mangrove function. This project aims to combine cutting-edge analytical and imaging techniques to assess contributions of atmospheric water sources to shoot-water balances, identify leaf traits associated with top-down rehydration, and determine the relative importance of different sources of water used by mangroves in maintenance of photosynthetic carbon assimilation along natural gradients in salinity and aridity. The capacity of shoots to absorb atmospheric water could profoundly affect the diversity, survival and productivity of mangroves where high soil salinity limits water uptake by roots, particularly during hot, dry conditions.Read moreRead less
Development of cryopreservation for high value provenance collections of recalcitrant plant species used in post-mining restoration. This project will develop new and innovative ways to store highly valued native plant germplasm at ultra cold temperatures (-196 °C, in liquid nitrogen) as a means to ensure that elite genotypes used in minesite restoration and critically endangered species are not lost forever to extinction. This project will be the first of its type in Australia utilising a multi ....Development of cryopreservation for high value provenance collections of recalcitrant plant species used in post-mining restoration. This project will develop new and innovative ways to store highly valued native plant germplasm at ultra cold temperatures (-196 °C, in liquid nitrogen) as a means to ensure that elite genotypes used in minesite restoration and critically endangered species are not lost forever to extinction. This project will be the first of its type in Australia utilising a multidisciplinary approach to answer key storage questions and will provide significant national benefits to conservation programs and landcare groups, providing these with additional resources to ensure the long-term survival of native plant germplasm collections. Read moreRead less
Potential of Corymbia torelliana hybrids for hardwood forestry and investigation of their seed dispersal by Trigona bees. Cadaghi (Corymbia torelliana) and their hybrids with spotted gums (C. variegata complex) have enormous potential for plantation forestry. These hybrids have many excellent features that make them exciting as hardwood species, such as resistence to disease, tolerance of marginal environments, good wood properties, and fast growth rates. This project will create hybrids between ....Potential of Corymbia torelliana hybrids for hardwood forestry and investigation of their seed dispersal by Trigona bees. Cadaghi (Corymbia torelliana) and their hybrids with spotted gums (C. variegata complex) have enormous potential for plantation forestry. These hybrids have many excellent features that make them exciting as hardwood species, such as resistence to disease, tolerance of marginal environments, good wood properties, and fast growth rates. This project will create hybrids between Corymbia torelliana and spotted gums to identify hybrids which are suitable for sustainable wood production on marginal agricultural lands.
An unusual feature of Cadaghi is that native Trigona bees disperse their seeds. This project will investigate this unique seed dispersal mechanism and identify features of hybrids that are not attractive to bees. This will prevent environmental problems by preventing hybrids from dispersing seeds, becoming weedy and harming the Trigona bees.
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Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a soluti ....Efficient organelle transformation. Chloroplasts and mitochondria are the powerhouses of plant and animal cells. Ability to express introduced genes in these organelles has enormous biotechnological potential in agriculture and medicine, but practical development has been almost stalled for 15 years by very low transformation efficiency. Plastid transformation is today routine only in tobacco; and mitochondrial transformation has been achieved only in yeasts and algae. We have developed a solution, and achieved the key technical requirements for proof of concept. This collaboration between industry, government and university partners will deliver key Australian-owned IP, for environmentally-friendly plant biofactories, and for treatment of mitochondrial genetic disorders.Read moreRead less
Crosstalk between branching and flowering regulatory pathways in shoot development. This project will explore how a newly discovered plant hormone communicates with other plant and environmental signals to regulate shoot branching. Understanding this process is an important step towards enhancing the yield, productivity and sustainability of commercially important plant species.
Australia's Distinctive Succulent Flora. The project plans to investigate why Australia, the driest vegetated continent, has no landscape dominated by large succulents but nevertheless supports a distinctive, diverse and widespread succulent flora. Focusing on terrestrial succulents and epiphytic orchids, the project plans to explore the evolution, assembly and biodiversity of Australia’s succulent flora, evaluating the roles of genetic composition, photosynthetic physiology, aridity, fire, soil ....Australia's Distinctive Succulent Flora. The project plans to investigate why Australia, the driest vegetated continent, has no landscape dominated by large succulents but nevertheless supports a distinctive, diverse and widespread succulent flora. Focusing on terrestrial succulents and epiphytic orchids, the project plans to explore the evolution, assembly and biodiversity of Australia’s succulent flora, evaluating the roles of genetic composition, photosynthetic physiology, aridity, fire, soil nutrients and salinity in its historical expansion, and assessing the resilience of the assemblages to changing climate. Of particular interest will be how the most water-use efficient type of photosynthesis, crassulacean acid metabolism, is expressed across the succulent landscape.Read moreRead less
The Role of Sugar Demand in Apical Dominance: Branching in a New Direction. For centuries, people around the world have made use of the knowledge that pruning the growing tips of shoots causes buds on the stem below to grow out into branches. Yields in crop, horticulture and forestry industries depend on shoot architecture. Since the discovery of auxin in the 1930s, shoot branching has been thought to be regulated by plant hormones. However, in this project we propose a new theory whereby shoot ....The Role of Sugar Demand in Apical Dominance: Branching in a New Direction. For centuries, people around the world have made use of the knowledge that pruning the growing tips of shoots causes buds on the stem below to grow out into branches. Yields in crop, horticulture and forestry industries depend on shoot architecture. Since the discovery of auxin in the 1930s, shoot branching has been thought to be regulated by plant hormones. However, in this project we propose a new theory whereby shoot tip demand for sugar is predominant in apical dominance; enhanced sucrose, a mobile sugar, is necessary and sufficient for the initial growth of buds. This project aims to expand this theory, revealing underlying components involved in sucrose action and describe how the network of sugar demand and hormones acts to control shoot architecture.Read moreRead less