Structural and functional investigations of the human transcription machinery by ion mobility-mass spectrometry. This project will apply emerging mass spectrometric technologies to gain previously inaccessible insight into human transcription factor proteins. This will reveal new avenues for intervention in human disease states related to aberrant gene expression, while developing innovative methods for the study of complex protein assemblies.
Three-dimensional structures, substrate specificities and catalytic mechanisms of polysaccharide synthases. Plant wall polysaccharides are extensively used for food and fibre, and are important dietary components in human nutrition. A precise knowledge of mechanisms used by plants to synthesize these polysaccharides is unavailable, but would reveal potential routes to manipulate their biosynthesis in important crop species. For example, the levels or structures of polysaccharides might be modif ....Three-dimensional structures, substrate specificities and catalytic mechanisms of polysaccharide synthases. Plant wall polysaccharides are extensively used for food and fibre, and are important dietary components in human nutrition. A precise knowledge of mechanisms used by plants to synthesize these polysaccharides is unavailable, but would reveal potential routes to manipulate their biosynthesis in important crop species. For example, the levels or structures of polysaccharides might be modified to improve their efficacy as anti-cancer agents in human diets, to enhance digestibility of animal stock feeds, or to synthesise pharmologically valuable compounds. Thus, economic, social and environmental benefits will flow to both producers and consumers. Read moreRead less
Structural and molecular studies of endocrine disruption in Australia fauna. Contamination of waterways with compounds that disrupt hormone (endocrine) function is a major environmental problem and threat to the health and fertility of animals. Specifically, we lack an understanding of how these potent endocrine disrupting compounds function in native species. Using an innovative combination of structural and molecular biology approaches we will elucidate the mechanisms of action of environmenta ....Structural and molecular studies of endocrine disruption in Australia fauna. Contamination of waterways with compounds that disrupt hormone (endocrine) function is a major environmental problem and threat to the health and fertility of animals. Specifically, we lack an understanding of how these potent endocrine disrupting compounds function in native species. Using an innovative combination of structural and molecular biology approaches we will elucidate the mechanisms of action of environmental endocrine disrupting compounds in native aquatic species - model fish and the platypus; and develop novel technologies for their detection. This work will provide an understanding of the environmental threat of these pollutants to our unique wildlife and will guide future waterway management. Read moreRead less
Human Leukocyte Antigen-A and -B regulation of Natural Killer cell function. The aim of this project is to determine how genetic variation in the genes encoding cell surface receptors expressed by innate lymphocytes and the molecules they recognise diversifies their capacity to sense and respond to infection. This knowledge is critical for understanding why there are intrinsic differences between individuals with respect to their capacity to respond to different types of infection and will ultim ....Human Leukocyte Antigen-A and -B regulation of Natural Killer cell function. The aim of this project is to determine how genetic variation in the genes encoding cell surface receptors expressed by innate lymphocytes and the molecules they recognise diversifies their capacity to sense and respond to infection. This knowledge is critical for understanding why there are intrinsic differences between individuals with respect to their capacity to respond to different types of infection and will ultimately inform our capacity to better deploy personalised medicines.Read moreRead less
Fibre reinforced polymer retrofitting of reinforced concrete flexural members. A cost-effective and durable technique for retrofitting reinforced concrete beams and slabs is to adhesively bond new advanced fibre reinforced polymer (FRP) composites to their surfaces. The potential plating market in Australia for bridges is $350 million, however, the technique can also be applied to strengthening other structures such as buildings. Tests have shown that FRP plates or sheets can debond prematurely, ....Fibre reinforced polymer retrofitting of reinforced concrete flexural members. A cost-effective and durable technique for retrofitting reinforced concrete beams and slabs is to adhesively bond new advanced fibre reinforced polymer (FRP) composites to their surfaces. The potential plating market in Australia for bridges is $350 million, however, the technique can also be applied to strengthening other structures such as buildings. Tests have shown that FRP plates or sheets can debond prematurely, which at present severely limits the application. The aim of this research project is to quantify the debonding mechanisms, strengths and ductilities of adhesively bonded FRP plated beams to provide design rules that can be used in practice.Read moreRead less
New biocatalysts for selective chemical oxidations under extreme conditions. This project will identify and design new enzyme biocatalysts which function under extreme conditions such as elevated temperature and high concentrations of peroxides. These enzymes will be sourced from microorganisms which are located in extreme biological environments e.g. hot springs (the so-called extremophiles). The expected outcome of this project are the identification of robust enzymes which can catalyse select ....New biocatalysts for selective chemical oxidations under extreme conditions. This project will identify and design new enzyme biocatalysts which function under extreme conditions such as elevated temperature and high concentrations of peroxides. These enzymes will be sourced from microorganisms which are located in extreme biological environments e.g. hot springs (the so-called extremophiles). The expected outcome of this project are the identification of robust enzymes which can catalyse selective oxidation reactions in complex organic molecules, such as steroids. The new biocatalysts developed in this project will have significant benefit in the development of new routes to access bespoke molecules of value in fine chemical synthesis and drug development.
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Nanoscale characterisation of the dynamics of artificial lipid membranes - model systems for drug binding studies. This project will see the development of artificial membranes replicating the physiological behaviour of cell membranes providing a novel platform for in vitro drug evaluation clearing the way for the development of effective new therapies with fewer side effects.
Earthquake protection of masonry buildings using fibre reinforced polymer strengthening. The importance of this research is highlighted, both nationally and internationally, by the work of the insurance industry which reported that the economic risk posed by a moderate earthquake in any of the capital cities in Australia is of the order of billions of dollars (Blong, 1993). For example, a 'design magnitude' earthquake in Sydney is predicted to cause, just in domestic construction, over $10 bill ....Earthquake protection of masonry buildings using fibre reinforced polymer strengthening. The importance of this research is highlighted, both nationally and internationally, by the work of the insurance industry which reported that the economic risk posed by a moderate earthquake in any of the capital cities in Australia is of the order of billions of dollars (Blong, 1993). For example, a 'design magnitude' earthquake in Sydney is predicted to cause, just in domestic construction, over $10 billion damage and more than 5000 deaths. Most of this damage would be associated with unreinforced masonry construction such as low-rise apartment buildings. The damage bill for the entire built environment (with business interruption costs) could easily be an order of magnitude greater.Read moreRead less
Ductile FRP retrofit of concrete frames subject to static and earthquake loading. Australia needs to spend at least $500 million annually on the repair and upgrading of concrete structures alone just to maintain our existing civil infrastructure. Furthermore, it has been estimated that more than 500 deaths and $10 billion damage will be caused by a moderate earthquake in Sydney or Melbourne. Most of this damage will be due to the collapse of buildings. This research will develop a new form of ....Ductile FRP retrofit of concrete frames subject to static and earthquake loading. Australia needs to spend at least $500 million annually on the repair and upgrading of concrete structures alone just to maintain our existing civil infrastructure. Furthermore, it has been estimated that more than 500 deaths and $10 billion damage will be caused by a moderate earthquake in Sydney or Melbourne. Most of this damage will be due to the collapse of buildings. This research will develop a new form of ductile plating that will reduce the cost of retrofitting concrete bridges and buildings. This project will also help train two PhD students and one Research Associate and further enhance the existing collaboration between Australia and Italy.Read moreRead less
A unified reinforced concrete model for flexure and shear. The catastrophic Minnesota River Bridge collapse in the USA in 2007 highlighted the importance of accurately assessing, maintaining, upgrading and prolonging the design life of our aging infrastructure. The problem, which is just as severe in Australia, is further exacerbated by increasing man-made (terrorist) hazards and natural hazards such as earthquake, tsunami, flood and bushfire. Reinforced concrete and composite steel-concrete st ....A unified reinforced concrete model for flexure and shear. The catastrophic Minnesota River Bridge collapse in the USA in 2007 highlighted the importance of accurately assessing, maintaining, upgrading and prolonging the design life of our aging infrastructure. The problem, which is just as severe in Australia, is further exacerbated by increasing man-made (terrorist) hazards and natural hazards such as earthquake, tsunami, flood and bushfire. Reinforced concrete and composite steel-concrete structures comprise a very large part of Australia's bridge and building infrastructure. This project will provide a safe and more economical tool for engineers to both extend the working-life of existing infrastructure and design new infrastructure.Read moreRead less