Molecular structure and function of the glycine receptor. This proposal will employ a cutting edge approach to reveal fundamental new insights into the ways that ligand-gated ion channels, and proteins in general, work. The new knowledge and technology developed here will broaden and strengthen Australia's research expertise across a number of basic scientific disciplines. The results will also have relevance to human health. Glycine receptors have an essential role in brain function and are ....Molecular structure and function of the glycine receptor. This proposal will employ a cutting edge approach to reveal fundamental new insights into the ways that ligand-gated ion channels, and proteins in general, work. The new knowledge and technology developed here will broaden and strengthen Australia's research expertise across a number of basic scientific disciplines. The results will also have relevance to human health. Glycine receptors have an essential role in brain function and are targets for anaesthetics and drugs of abuse. GlyRs are also important in modulating pain sensation by the brain. New insights into how natural agonists and drugs affect ion channel structure and function may lead to novel therapeutic opportunities and improved drug structure predictions.Read moreRead less
Membrane proteins in innate immunity. The application of smarter and faster methods for understanding membrane proteins, targets of most drugs, is vital to a knowledge-based economy and a healthy society. The long-term benefits will include fundamental new knowledge on immunity, and implementation of new approaches that streamline costs and efforts of challenging, high-impact research.
Structure and function of human zinc transporter membrane proteins. The aim of this project is to create fundamental new knowledge on how important mammalian membrane proteins operate. Membrane proteins are key drug targets and are significantly under-represented in structural databases. The project plans to combine innovative membrane protein screening technology with gene expression, structural biology, biophysics and cell biology. The project outcomes may elucidate specific molecular mechanis ....Structure and function of human zinc transporter membrane proteins. The aim of this project is to create fundamental new knowledge on how important mammalian membrane proteins operate. Membrane proteins are key drug targets and are significantly under-represented in structural databases. The project plans to combine innovative membrane protein screening technology with gene expression, structural biology, biophysics and cell biology. The project outcomes may elucidate specific molecular mechanisms underpinning the essential biological process of zinc homeostasis.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100149
Funder
Australian Research Council
Funding Amount
$590,000.00
Summary
Reaching new heights in high-resolution electron microscopy . High-resolution electron microscopy (EM): Direct electron detection cameras are a recent technological breakthrough delivering one of the greatest single advancements to the field of molecular cryo-EM. The aim of this project is to enable a 'first of a kind' cryo-EM platform in Australia enabling high-throughput atomic resolution protein structure determination. This will be achieved by integrating a state-of-the-art Gatan K2 Summit D ....Reaching new heights in high-resolution electron microscopy . High-resolution electron microscopy (EM): Direct electron detection cameras are a recent technological breakthrough delivering one of the greatest single advancements to the field of molecular cryo-EM. The aim of this project is to enable a 'first of a kind' cryo-EM platform in Australia enabling high-throughput atomic resolution protein structure determination. This will be achieved by integrating a state-of-the-art Gatan K2 Summit Direct Electron Detection camera system into the established cryo-EM facility managed by the University of Queensland node of the Australian Microscopy and Microanalysis Facility. This will offer unique and significantly improved capabilities for atomic resolution protein structure analysis, and will support a broad range of projects across the biological sciences.Read moreRead less
Soil microbial ecology and function in forest nutrient cycling: Improving the understanding and management with bio-molecular and stable isotope techniques. An improved understanding of soil microbial ecology and function is required to advance our knowledge and management of important carbon and nutrient cycling processes underpinning forest productivity and sustainability. Recent advances in bio-molecular techniques and application of stable isotope 13C and 15N methodologies have highlighted t ....Soil microbial ecology and function in forest nutrient cycling: Improving the understanding and management with bio-molecular and stable isotope techniques. An improved understanding of soil microbial ecology and function is required to advance our knowledge and management of important carbon and nutrient cycling processes underpinning forest productivity and sustainability. Recent advances in bio-molecular techniques and application of stable isotope 13C and 15N methodologies have highlighted the exciting opportunities and potential for studying soil microbial ecology and function in forest nutrient cycling. These innovative bio-molecular and stable isotope techniques will be effectively used to examine the impacts of management practices and global change on the soil carbon and nutrient cycling processes for enhancing both productivity and environmental benefits of forest ecosystems.Read moreRead less
Structural insights of virus-glycan interactions. Influenza virus, rotavirus and Dengue virus infect the body by adhering to certain types of sugars on the human cell surface. This project will develop a detailed structural understanding of how viruses interact with those sugar molecules for the development of novel drugs and vaccines to combat influenza and rotaviral infections.
Unravelling the structural complexity of ancient Australian arthropod venoms. Animal venoms contain a complex mix of molecules that have evolved over millions of years to target various biological processes. Their exquisite specificity and potent activity has made them an attractive source for development as therapeutic drugs with fewer side effects or as environmentally friendly insecticides. This project aims to for the first time explore the three-dimensional structure of all known classes of ....Unravelling the structural complexity of ancient Australian arthropod venoms. Animal venoms contain a complex mix of molecules that have evolved over millions of years to target various biological processes. Their exquisite specificity and potent activity has made them an attractive source for development as therapeutic drugs with fewer side effects or as environmentally friendly insecticides. This project aims to for the first time explore the three-dimensional structure of all known classes of toxins in the venom of two ancient Australian arthropods (spiders and centipedes). This will provide a comprehensive overview of these venoms and provide opportunities in engineering new classes of venom based drugs and insecticides. Read moreRead less
Automated structural analysis of proteins by nuclear magnetic resonance (ASAP-NMR): a leap forward in structural studies of proteins using NMR spectroscopy. Proteins form the machinery that makes life possible, and this research will revolutionise study of their three-dimensional structure by making the process faster and cheaper. This approach will be applied to study proteins isolated from animal venoms in order to identify and develop novel pharmaceuticals and bio-insecticides.
Elucidation of structure-function relationships in biological systems utilising advanced electron spin resonance. The human growth hormone receptor and mammalian P450 enzymes are implicated in numerous human disorders and the key to treatment is the rational design and delivery of drugs. This project will require a comprehensive understanding of the biological structure-function relationship which we will acquire using high-resolution electron spin resonance spectroscopy.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100128
Funder
Australian Research Council
Funding Amount
$237,000.00
Summary
Overcoming membrane protein research roadblocks: A Queensland facility for membrane protein production and crystallisation. Many drugs target specialised molecules called membrane proteins that reside at the interface between cells and the cells' environment. This membrane protein production facility will address significant challenges in the production and analysis of membrane proteins, which will enable a better understanding of how they function and provide a platform for the design of new an ....Overcoming membrane protein research roadblocks: A Queensland facility for membrane protein production and crystallisation. Many drugs target specialised molecules called membrane proteins that reside at the interface between cells and the cells' environment. This membrane protein production facility will address significant challenges in the production and analysis of membrane proteins, which will enable a better understanding of how they function and provide a platform for the design of new and better drugs. Read moreRead less