ARC Centre for Kangaroo Genome. In this Australian-led Kangaroo Genome Project, we will map and characterize the tammar wallaby genome at the molecular level. Marsupial genomes are uniquely valuable because they provide comparisons that reveal new human genes, regulatory sequences and marsupial-specific genes. These will deliver new products and information useful for medicine, industry, agriculture and conservation. We will construct integrated genetic and physical maps of the genome, clone the ....ARC Centre for Kangaroo Genome. In this Australian-led Kangaroo Genome Project, we will map and characterize the tammar wallaby genome at the molecular level. Marsupial genomes are uniquely valuable because they provide comparisons that reveal new human genes, regulatory sequences and marsupial-specific genes. These will deliver new products and information useful for medicine, industry, agriculture and conservation. We will construct integrated genetic and physical maps of the genome, clone the whole genome as large inserts in BAC vectors, and build a "golden path" with minimal overlap. We will construct libraries of expressed genes from tammar tissues and array them for use in analysing gene expression.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561030
Funder
Australian Research Council
Funding Amount
$441,100.00
Summary
Developmental Imaging Facility. This application seeks to establish a facility to undertake expression profiling in vertebrate tissues on a genomic scale and at the highest resolution. Undertaking large scale projects of this nature requires specialised robotics and dedicated infrastructure for microscopy and tissue preparation. This facility will be the first of its type in Australia will permit researchers to perform genomic scale in situ screens, many as part of large international initiative ....Developmental Imaging Facility. This application seeks to establish a facility to undertake expression profiling in vertebrate tissues on a genomic scale and at the highest resolution. Undertaking large scale projects of this nature requires specialised robotics and dedicated infrastructure for microscopy and tissue preparation. This facility will be the first of its type in Australia will permit researchers to perform genomic scale in situ screens, many as part of large international initiatives in developmental and cellular biology. This large-scale, high-resolution expression profiling infrastructure is required to maintain international competitiveness and will dramatically improve our gene discovery, functional assessment and understanding of vertebrate development.Read moreRead less
Comprehensive characterisation of DNA demethylation pathways in vivo. This project aims to provide a better understanding of the roles that DNA methylation plays during embryonic development. DNA methylation is a major regulatory mark present in vertebrate genomes. It is well established that the genomic patterns of DNA methylation are being actively remodelled during vertebrate embryogenesis. Nevertheless, it remains unclear how these events impact gene regulation and embryonic development itse ....Comprehensive characterisation of DNA demethylation pathways in vivo. This project aims to provide a better understanding of the roles that DNA methylation plays during embryonic development. DNA methylation is a major regulatory mark present in vertebrate genomes. It is well established that the genomic patterns of DNA methylation are being actively remodelled during vertebrate embryogenesis. Nevertheless, it remains unclear how these events impact gene regulation and embryonic development itself. This project expects to unravel the functional contributions of DNA methylation to vertebrate embryogenesis by using latest cutting-edge genomics techniques. The project will be carried out on the highly tractable zebrafish model system which allows for easy genetic manipulation of the desired sequences. This project aims to provide a better understanding of embryonic development of vertebrates, including humans.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0560987
Funder
Australian Research Council
Funding Amount
$156,697.00
Summary
Robust High Resolution Gene and Protein Expression Analysis Facilities in WA. Biological research is playing an increasingly important role in keeping agriculture internationally competitive and helping to unravel the basic mechanisms underpinning plant and animal health. This collaborative research equipment will greatly enhance and extend our existing functional genomic facilities in WA, allowing robust pre-fractionation of samples for directed proteomic analysis within complex systems and al ....Robust High Resolution Gene and Protein Expression Analysis Facilities in WA. Biological research is playing an increasingly important role in keeping agriculture internationally competitive and helping to unravel the basic mechanisms underpinning plant and animal health. This collaborative research equipment will greatly enhance and extend our existing functional genomic facilities in WA, allowing robust pre-fractionation of samples for directed proteomic analysis within complex systems and allowing accurate and sensitive measurement of gene expression. Both of these are critical for analysis of low abundance components involved in signalling and regulatory functions in biological samples.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453722
Funder
Australian Research Council
Funding Amount
$385,240.00
Summary
Collaborative Genomics, Proteomics and Metabolomics Facility for Western Australia. Plant and animal agriculture in Western Australia contributes $6billion per annum to the nation. Biotechnology is playing an increasingly important role in keeping agriculture internationally competitive, and requires investment in platform technologies to underpin basic and applied research. This collaborative project will provide state-of-the-art equipment and extend existing joint facilities that will enable ....Collaborative Genomics, Proteomics and Metabolomics Facility for Western Australia. Plant and animal agriculture in Western Australia contributes $6billion per annum to the nation. Biotechnology is playing an increasingly important role in keeping agriculture internationally competitive, and requires investment in platform technologies to underpin basic and applied research. This collaborative project will provide state-of-the-art equipment and extend existing joint facilities that will enable WA researchers to carry out high quality research on genomics, proteomics and the metabolic functioning of plants and animals. This will generate new knowledge, provide advanced training and help ensure that Australian R&D in agricultural biotechnology stays at the forefront and benefits the nation.Read moreRead less
Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family protei ....Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family proteins as specific effectors. This project will address a new paradigm in genetics that is likely to underpin development.Read moreRead less
Sex determination in dragons: Genetics, epigenetics and environment. This project aims to discover the master sex-determining gene in a reptile, how that gene is differentially regulated in males and females and by temperature, and to identify evolutionary drivers of transitions between genetic and environmental sex determination. In many reptiles, like mammals, chromosomes determine sex. In others, the temperature at which their eggs are incubated determines sex. This project will study how tem ....Sex determination in dragons: Genetics, epigenetics and environment. This project aims to discover the master sex-determining gene in a reptile, how that gene is differentially regulated in males and females and by temperature, and to identify evolutionary drivers of transitions between genetic and environmental sex determination. In many reptiles, like mammals, chromosomes determine sex. In others, the temperature at which their eggs are incubated determines sex. This project will study how temperature reverses chromosomal sex determination in dragon lizards. This could show how climatic extremes affect the biology of climate sensitive reptiles, and understand their vulnerability to climate change.Read moreRead less
Lived experiences of treatment for hepatitis C in Australia. This project aims to support uptake of new hepatitis C treatments. With the introduction of new treatments in 2016, the Australian Government adopted the WHO’s goal of eliminating the disease by 2030. While early treatment rates were high, they have since plateaued, with stigma and poor information considered key obstacles. This project will generate new knowledge on treatment decisions and experiences, using a proven qualitative metho ....Lived experiences of treatment for hepatitis C in Australia. This project aims to support uptake of new hepatitis C treatments. With the introduction of new treatments in 2016, the Australian Government adopted the WHO’s goal of eliminating the disease by 2030. While early treatment rates were high, they have since plateaued, with stigma and poor information considered key obstacles. This project will generate new knowledge on treatment decisions and experiences, using a proven qualitative methodology. In doing so, it will produce a website covering personal experiences of treatment, issues in treatment decision-making, and advice on enhancing life on treatment and after. It will tackle hepatitis C-related stigma, and inform and benefit potential treatment users, families and relevant professionals.
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Discovery Early Career Researcher Award - Grant ID: DE140101962
Funder
Australian Research Council
Funding Amount
$395,220.00
Summary
Functional epigenomics interrogation of DNA methylation dynamics during vertebrate development and evolution. DNA methylation (mC) is an epigenetic signal essential for the maintenance of correct gene expression patterns. To investigate the causal relationships between mC and transcription during vertebrate embryonic development and evolution, this project will perform high-resolution mC profiling at different stages of teleost, amphibian and mammalian development. Highly conserved and syntenic, ....Functional epigenomics interrogation of DNA methylation dynamics during vertebrate development and evolution. DNA methylation (mC) is an epigenetic signal essential for the maintenance of correct gene expression patterns. To investigate the causal relationships between mC and transcription during vertebrate embryonic development and evolution, this project will perform high-resolution mC profiling at different stages of teleost, amphibian and mammalian development. Highly conserved and syntenic, methylated sequences will then be used as baits in proteomics screens to identify novel 5mC 'readers'. The generation of genomic profiles of mC 'readers' and their integration with developmental mC maps will reveal transient epigenome dynamics during vertebrate embryogenesis and provide new insights into the conservation of these crucial developmental mechanisms.Read moreRead less
The Epigenetics of Sex in the Dragon. Genetic codes do not directly translate to phenotypes -- environment acts through epigenetics to modify development. We use advanced molecular techniques to examine how epigenetics responds to temperature to reverse sex in our novel animal model, the dragon lizard. How does the cell sense temperature? Once the extrinsic signal is captured, how does it influence chromatin modification to release or suppress key genes in the sex differentiation pathway? Which ....The Epigenetics of Sex in the Dragon. Genetic codes do not directly translate to phenotypes -- environment acts through epigenetics to modify development. We use advanced molecular techniques to examine how epigenetics responds to temperature to reverse sex in our novel animal model, the dragon lizard. How does the cell sense temperature? Once the extrinsic signal is captured, how does it influence chromatin modification to release or suppress key genes in the sex differentiation pathway? Which sex genes are targets? Epigenetic enzymes are astonishingly conserved, providing exciting opportunities to draw from human systems to unravel novel signatures of temperature-induced sex switching in reptiles. This project will advance knowledge of developmental programming generally.Read moreRead less