Understanding an exotic disease: Initiation of sex and infection by the sugarcane smut Ustilago scitaminea. Australian sugar exports generate almost $2 billion in annual sales, making the sugar industry a critical facet of the Australian economy. In 2006, Australia's primary sugar producing region came under threat when an outbreak of sugarcane smut caused by the fungus Ustilago scitaminea first appeared in Queensland. Management of this potentially devastating disease has focused on breeding pr ....Understanding an exotic disease: Initiation of sex and infection by the sugarcane smut Ustilago scitaminea. Australian sugar exports generate almost $2 billion in annual sales, making the sugar industry a critical facet of the Australian economy. In 2006, Australia's primary sugar producing region came under threat when an outbreak of sugarcane smut caused by the fungus Ustilago scitaminea first appeared in Queensland. Management of this potentially devastating disease has focused on breeding programmes aimed at developing resistant sugarcane cultivars, a complex process hampered by a lack of information about the mechanisms of smut resistance. Our research will provide key insight into the mechanisms by which U. scitaminea infects sugarcane, directing future breeding efforts and protecting this valuable industry against further outbreaks.Read moreRead less
A New Window into Transgene Silencing in Plants: mechanisms of copy-number independent, 5' sequence dependent, post-transcriptional silencing in a complex polyploid. Silencing of introduced genes is a major problem limiting plant molecular improvement. Sugarcane, a complex polyploid, shows the most efficient transgene silencing ever observed in plants. Silencing operates on the RNA, depends on the upstream sequence of the gene, and is independent of copy number. Other plant species develop endop ....A New Window into Transgene Silencing in Plants: mechanisms of copy-number independent, 5' sequence dependent, post-transcriptional silencing in a complex polyploid. Silencing of introduced genes is a major problem limiting plant molecular improvement. Sugarcane, a complex polyploid, shows the most efficient transgene silencing ever observed in plants. Silencing operates on the RNA, depends on the upstream sequence of the gene, and is independent of copy number. Other plant species develop endopolyploidy with age, and show unpredictable or patchy silencing. We speculate that differential silencing is a natural control mechanism in the exploitation of polyploidy in plants. The sugarcane system provides an exceptional opportunity to identify the sequences that trigger and protect from silencing, and to develop approaches to avoid the problem.Read moreRead less
Modification of lignin biosynthesis in sugarcane for the improved efficiency of pre-treatment in ethanol production. Sugarcane is one of Australia's most important rural industries. However, as a single product industry, declining sugar prices threaten the industry's long term economic sustainability unless alternative markets for sugarcane are created. Utilising the sugarcane waste for cellulosic ethanol would provide a new revenue stream, injecting life into the Australian sugarcane industry. ....Modification of lignin biosynthesis in sugarcane for the improved efficiency of pre-treatment in ethanol production. Sugarcane is one of Australia's most important rural industries. However, as a single product industry, declining sugar prices threaten the industry's long term economic sustainability unless alternative markets for sugarcane are created. Utilising the sugarcane waste for cellulosic ethanol would provide a new revenue stream, injecting life into the Australian sugarcane industry. In addition, cellulosic ethanol from sugarcane has the potential to substantially decrease the cost of biofuel production and significantly reduce greenhouse gas emissions. The research proposed here will advance our ability to improve sugarcane through biotechnology.Read moreRead less
Plant transformation: exploiting anti-apoptosis genes for very high efficiency transformation. Crop improvement through genetic modification depends on the ability to transform target species. The most desirable method is Agrobacterium mediated transformation. However, plant species and cultivars differ significantly in their ability to be efficiently transformed by Agrobacterium. This is particularly true for the economically important cereals. We have discovered that anti-apoptosis genes, whic ....Plant transformation: exploiting anti-apoptosis genes for very high efficiency transformation. Crop improvement through genetic modification depends on the ability to transform target species. The most desirable method is Agrobacterium mediated transformation. However, plant species and cultivars differ significantly in their ability to be efficiently transformed by Agrobacterium. This is particularly true for the economically important cereals. We have discovered that anti-apoptosis genes, which inhibit programmed cell death, dramatically increase the Agrobacterium transformation efficiency in bananas and sugarcane. We will utilise this information and develop the use of these genes to increase the efficiency of transformation in those crops and cultivars that are difficult to transform using Agrobacterium.Read moreRead less
Modelling soft filled viscoelastic solids. Wheat flour doughs are made in vast quantities daily, and improvements in processing, especially in sheeting (rolling) and extrusion are needed. To enable this we will create a novel mathematical description of the material which will be useful for predicting the shapes of processed products. Wheat breeding cycles can be shortened by using the model rheological parameters, since baking quality is closely linked to dough rheology. A number of Australian ....Modelling soft filled viscoelastic solids. Wheat flour doughs are made in vast quantities daily, and improvements in processing, especially in sheeting (rolling) and extrusion are needed. To enable this we will create a novel mathematical description of the material which will be useful for predicting the shapes of processed products. Wheat breeding cycles can be shortened by using the model rheological parameters, since baking quality is closely linked to dough rheology. A number of Australian wheats will be considered and their rheologies will be compared.Read moreRead less
Experimental and Numerical Investigation of Granular Flow in Hoppers. Handling and processing of bulk solids such as coal and metal ores perform a key function in many industries, and hoppers are the most common devices for storage and discharge of granular materials in this field. The proper design of this equipment is very important. This project provides a systematic investigation of granular flow in hoppers, which is essential in order to avoid structure failures and associated costs. It wil ....Experimental and Numerical Investigation of Granular Flow in Hoppers. Handling and processing of bulk solids such as coal and metal ores perform a key function in many industries, and hoppers are the most common devices for storage and discharge of granular materials in this field. The proper design of this equipment is very important. This project provides a systematic investigation of granular flow in hoppers, which is essential in order to avoid structure failures and associated costs. It will lead to a fundamental understanding of relevant industrial processes, improvement of their efficiency, and hence improved competitiveness of Australian mining/mineral/metallurgical industries.Read moreRead less
Establishing the relations between starch nano- and mesostructure and macroscopic physical properties. Starch is the major energy component within human diets, and the most abundant polymer that can be readily extracted from annual crop plants, leading to many actual and potential industrial applications. There are major opportunities to optimise the nutritional value of starches in the human diet, and to enhance the properties of extracted starches as renewable alternatives to petrochemical pol ....Establishing the relations between starch nano- and mesostructure and macroscopic physical properties. Starch is the major energy component within human diets, and the most abundant polymer that can be readily extracted from annual crop plants, leading to many actual and potential industrial applications. There are major opportunities to optimise the nutritional value of starches in the human diet, and to enhance the properties of extracted starches as renewable alternatives to petrochemical polymers. This project will open up our understanding of the structure of starch polymers and show how this relates to important properties such as enzyme digestibility rates, leading to new opportunities for public health and commercial benefits.Read moreRead less
Targeting and stabilizing proteins in sugar storage vacuoles for metabolic engineering in sugarcane. We have isolated a novel gene for an enzyme that efficiently converts sucrose into a product of much higher value. We have shown that the enzyme functions in sugarcane, a first example of the potential for new biosynthetic capacities in this highly productive crop. Because 90% of stored sucrose is in specialized vacuoles, the enzyme needs to be directed into these vacuoles, and made stable and ac ....Targeting and stabilizing proteins in sugar storage vacuoles for metabolic engineering in sugarcane. We have isolated a novel gene for an enzyme that efficiently converts sucrose into a product of much higher value. We have shown that the enzyme functions in sugarcane, a first example of the potential for new biosynthetic capacities in this highly productive crop. Because 90% of stored sucrose is in specialized vacuoles, the enzyme needs to be directed into these vacuoles, and made stable and active there. This is feasible by building on recent discoveries about vacuolar targeting in plants. The outputs include scientific understanding to underpin metabolic engineering in plants, and a profitable high-technology export industry for Australia.Read moreRead less
Seeing the discrete in a continuum: an integrated numerical-rheological-experimental approach towards high resolution micromechanical continuum models of granular media. Processes involving granular materials are complex and rarely reach more than 60% of the design capacity, due to inadequate understanding of granular rheology. The short term benefits of the proposed project are: improved insights on the rheology of granular media; experimentally validated micromechanical constitutive models ....Seeing the discrete in a continuum: an integrated numerical-rheological-experimental approach towards high resolution micromechanical continuum models of granular media. Processes involving granular materials are complex and rarely reach more than 60% of the design capacity, due to inadequate understanding of granular rheology. The short term benefits of the proposed project are: improved insights on the rheology of granular media; experimentally validated micromechanical constitutive models with unmatched predictive capabilities; modelling techniques in the analysis of multiscale processes, germane to the Science of Complex Materials. The long term benefits are models of the required reliability for computer-aided design, production and management of particulate systems. These simulation tools will enhance Australia's competitive edge in the multi-billion dollar particulate and geotechnical industries.Read moreRead less
Cellulose-based composites as models for primary plant cell walls of cereals and grasses. Cereals and grasses are the lynchpins of the Australian Agri-Food industry. Cell walls provide shape, form and barrier properties to the plant and are the basis for both post-harvest mechanical properties and direct nutritional benefits. There is as yet no validated model for the molecular assembly, architecture and mechanical behaviour of cereal/grass cell walls. This project aims to derive such a model, s ....Cellulose-based composites as models for primary plant cell walls of cereals and grasses. Cereals and grasses are the lynchpins of the Australian Agri-Food industry. Cell walls provide shape, form and barrier properties to the plant and are the basis for both post-harvest mechanical properties and direct nutritional benefits. There is as yet no validated model for the molecular assembly, architecture and mechanical behaviour of cereal/grass cell walls. This project aims to derive such a model, so that predictions can be made concerning the effects of tailoring either plant composition (e.g. at the gene level) or post-harvest treatment in order to achieve desired plant or food properties. The findings will also be relevant to understanding how individual features of cell walls affect digestibility and nutritional properties.Read moreRead less