Buried treasure: bioactive plant seed proteins evolving inside hosts. This project aims to examine how evolution in plants shortcuts the creation of new proteins by burying one within another. Scientists now realise that new genes and proteins appear frequently. A recent discovery in plant seeds involves DNA sequence insertions in a gene that makes two proteins instead of one. This project will reveal a new family of buried seed proteins, determine the rules for burying them and search plants fo ....Buried treasure: bioactive plant seed proteins evolving inside hosts. This project aims to examine how evolution in plants shortcuts the creation of new proteins by burying one within another. Scientists now realise that new genes and proteins appear frequently. A recent discovery in plant seeds involves DNA sequence insertions in a gene that makes two proteins instead of one. This project will reveal a new family of buried seed proteins, determine the rules for burying them and search plants for new examples. The first examples from plants create strongly bioactive products so the ability to dig for similar plant events will reveal new and bioactive natural products with biomedical and biotechnology applications.Read moreRead less
Revealing Enigma of Salt Bladders to Help Crops Cope with Salinity. In this project, the key transport systems mediating salt sequestration in halophytes are planned to be characterised and linked with cell genetic and metabolic profiles. Salinity is a major environmental hurdle affecting crop production around the world. Halophytes (naturally salt-loving plants) use specialised structures, called salt bladders, to sequester excessive salt outside their metabolically active parts. This feature i ....Revealing Enigma of Salt Bladders to Help Crops Cope with Salinity. In this project, the key transport systems mediating salt sequestration in halophytes are planned to be characterised and linked with cell genetic and metabolic profiles. Salinity is a major environmental hurdle affecting crop production around the world. Halophytes (naturally salt-loving plants) use specialised structures, called salt bladders, to sequester excessive salt outside their metabolically active parts. This feature is not utilised by crops however, and no information is available about the molecular mechanisms by which salt is pumped into bladder cells. This knowledge will allow breeders to utilise this, previously unexplored, trait to improve crop performance under conditions of salinity.Read moreRead less
A new signalling component in shoot architecture: trehalose 6-phosphate. This project aims to investigate the role of a new signalling pathway involved in shoot branching. New knowledge is expected on how plants regulate shoot branching via sugar or hormone levels and/or signalling. The aims to build on recent finding that trehalose 6-phosphate (Tre6P) promotes shoot branching and to investigate whether sucrose acts via Tre6P and what role sucrose and Tre6P have compared with plant hormones. S ....A new signalling component in shoot architecture: trehalose 6-phosphate. This project aims to investigate the role of a new signalling pathway involved in shoot branching. New knowledge is expected on how plants regulate shoot branching via sugar or hormone levels and/or signalling. The aims to build on recent finding that trehalose 6-phosphate (Tre6P) promotes shoot branching and to investigate whether sucrose acts via Tre6P and what role sucrose and Tre6P have compared with plant hormones. Significant benefits may include new genetic or management strategies to modify shoot architecture.Read moreRead less
New crop on the block: The genetic control of hempseed nutritional quality. Hempseed, which is rich in polyunsaturated oils and high-quality protein, is emerging as a functional food crop across Australia. However, very little is known about the genetic control of oil and protein contents and composition, crucial characteristics for the optimization of hempseed productivity and quality for the Australian industry. A unique genetic resource of 120 diverse hemp accessions, many provided by our ind ....New crop on the block: The genetic control of hempseed nutritional quality. Hempseed, which is rich in polyunsaturated oils and high-quality protein, is emerging as a functional food crop across Australia. However, very little is known about the genetic control of oil and protein contents and composition, crucial characteristics for the optimization of hempseed productivity and quality for the Australian industry. A unique genetic resource of 120 diverse hemp accessions, many provided by our industry partner, will be used to define the genetics underpinning nutritional variation and associated genotype-by-environment interactions. This fundamental knowledge will lay the foundation for targeted breeding and best management practice, for the benefit of farmers, the hemp industry and health-conscious consumers.Read moreRead less
Harnessing peptide hormone outputs to improve root nodulation’s efficacy. This project aims to transform our understanding of symbiotic nitrogen fixation in legume root nodules. Root nodulation sustainably fixes sizeable amounts of nitrogen to boost crop production worldwide yet its utilisation is waning in favour of using nitrogen fertilisers. The project applies cutting-edge tools to define how two hormone systems boost and limit nitrogen fixation, respectively. The project expects to reveal w ....Harnessing peptide hormone outputs to improve root nodulation’s efficacy. This project aims to transform our understanding of symbiotic nitrogen fixation in legume root nodules. Root nodulation sustainably fixes sizeable amounts of nitrogen to boost crop production worldwide yet its utilisation is waning in favour of using nitrogen fertilisers. The project applies cutting-edge tools to define how two hormone systems boost and limit nitrogen fixation, respectively. The project expects to reveal ways to reconfigure these hormone outputs to improve nodule number and the efficacy of nitrogen fixation. The findings will benefit agriculture by reducing the reliance on costly nitrogen fertilisers, thus mitigating the huge environmental damage they cause, and will provide more sustainable ways to ensure food security.Read moreRead less
A new route to controlling root system architecture and drought tolerance. This project aims to transform our understanding of the relationship between root architecture and water and nitrogen acquisition, factors critical to determining yield. We have discovered that mutants affected in a peptide hormone receptor have unique root architectural features relevant to acquiring water and nitrogen. The mutants are drought tolerant and their roots are nitrate insensitive. The project aims to define t ....A new route to controlling root system architecture and drought tolerance. This project aims to transform our understanding of the relationship between root architecture and water and nitrogen acquisition, factors critical to determining yield. We have discovered that mutants affected in a peptide hormone receptor have unique root architectural features relevant to acquiring water and nitrogen. The mutants are drought tolerant and their roots are nitrate insensitive. The project aims to define the receptor’s genetic outputs and expects to uncover new ways to improve water and nitrate acquisition and determine if our findings apply to crops. The application of these findings will reduce the severe environmental damage caused by poor nitrogen fertiliser uptake and provide sustainable ways to ensure food security.Read moreRead less
REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespirat ....REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespiration and nitrogen assimilation. It will exploit stable isotopes to quantify metabolic partitioning and show coordination between major processes. It will establish key mechanisms by which respiration dictates plant carbon balance and contributes to identifying metabolic bottle-necks in plant primary production.Read moreRead less
Stomata functioning in halophytes for improved plant stress tolerance. This project aims to determine how halophytes balance photosynthesis and water loss under extreme soil conditions. Salinity and drought affect crop production. Plants’ ability to balance carbon dioxide uptake and water loss through stomata determines how they cope with stressors. Halophytes can achieve this balance at conditions that normally kill 99% of crops, but how they do so is unknown. This project will characterise the ....Stomata functioning in halophytes for improved plant stress tolerance. This project aims to determine how halophytes balance photosynthesis and water loss under extreme soil conditions. Salinity and drought affect crop production. Plants’ ability to balance carbon dioxide uptake and water loss through stomata determines how they cope with stressors. Halophytes can achieve this balance at conditions that normally kill 99% of crops, but how they do so is unknown. This project will characterise the transport systems mediating stomata function in halophytes and contribute to understanding the molecular and physiological basis of their operation. This should allow breeders to use this trait to improve crop performance under conditions of extreme salinity and drought.Read moreRead less
How scissors learn to glue: the catalysis of ligation by proteases. This project proposes to study protein-cutting enzymes from plants that are drawn into biosyntheses where they paradoxically perform protein-joining (ligation) reactions. Enzymes are everywhere, from detergents to digestion to detoxifying drugs. Industry uses artificial evolution to improve enzymes or create enzymes with new activities. By exploring the changes that allowed one such cutting enzyme to ligate, the knowledge acquir ....How scissors learn to glue: the catalysis of ligation by proteases. This project proposes to study protein-cutting enzymes from plants that are drawn into biosyntheses where they paradoxically perform protein-joining (ligation) reactions. Enzymes are everywhere, from detergents to digestion to detoxifying drugs. Industry uses artificial evolution to improve enzymes or create enzymes with new activities. By exploring the changes that allowed one such cutting enzyme to ligate, the knowledge acquired may enable protein engineering to develop designer enzymes with enhanced or suppressed ligating ability. Although protein evolution can be studied with artificial systems, natural systems are extremely valuable. Insights from studying this natural evolution of ligation may test hypotheses developed with data from artificial evolution.Read moreRead less
How do plant roots align nitrogen uptake to soil opportunities? Improved nitrogen use efficiency (NUE) in crop plants is required to achieve sustainable plant agriculture practices that maximise productivity while minimising nitrogen fertiliser-dependent pollution. Current high-input monoculture plant production systems suffer from poor NUE and can contribute to local and global nitrogen pollution outcomes. Improving how plants manage their nitrogen uptake will improve NUE and help support Aust ....How do plant roots align nitrogen uptake to soil opportunities? Improved nitrogen use efficiency (NUE) in crop plants is required to achieve sustainable plant agriculture practices that maximise productivity while minimising nitrogen fertiliser-dependent pollution. Current high-input monoculture plant production systems suffer from poor NUE and can contribute to local and global nitrogen pollution outcomes. Improving how plants manage their nitrogen uptake will improve NUE and help support Australian plant agriculture. This project will investigate novel technologies that re-engineer nitrate transport activity. The project will also investigate the biochemical and molecular links between nitrogen uptake on root development required for improved plant growth.Read moreRead less