Controlling the adhesome to regulate cell fate on biomaterials. Mesenchymal stem cell-based tissue engineering practices are hampered worldwide by the lack of appreciation and understanding of the matrix-mediated cues that must be provided during adhesion and spreading to drive cells to definitive tissue end points. This project will address these knowledge deficiencies by combining high throughput array technologies, a set of tailorable self-assembling biomaterials and real-time biosensors to r ....Controlling the adhesome to regulate cell fate on biomaterials. Mesenchymal stem cell-based tissue engineering practices are hampered worldwide by the lack of appreciation and understanding of the matrix-mediated cues that must be provided during adhesion and spreading to drive cells to definitive tissue end points. This project will address these knowledge deficiencies by combining high throughput array technologies, a set of tailorable self-assembling biomaterials and real-time biosensors to rapidly, at high resolution, elucidate how mechanotransductive cues determine the fate choice of mesenchymal stem cells, and furthermore, how to manipulate them with smart biomaterial design to achieve desired outcomes for tissue engineering. Read moreRead less
Bone tissue engineering using innovative tubular dual-layered nanofiber meshes. Lifetime risks for long-bone fractures in Caucasians over the age of 50 are 17 per cent for women and 6 per cent for men. A clear therapeutic need exists to address the ever-increasing problems of diminished productivity and reduced quality of life associated with bone disorders as the population ages. To address this challenge, the project’s multidisciplinary, international team will develop technologies to heal tib ....Bone tissue engineering using innovative tubular dual-layered nanofiber meshes. Lifetime risks for long-bone fractures in Caucasians over the age of 50 are 17 per cent for women and 6 per cent for men. A clear therapeutic need exists to address the ever-increasing problems of diminished productivity and reduced quality of life associated with bone disorders as the population ages. To address this challenge, the project’s multidisciplinary, international team will develop technologies to heal tibial defects. Furthermore, it will establish Australia's prominence in the tissue engineering field, training the next generation of young scientists and engineers. This technology will be of interest to numerous research groups and companies worldwide and will foster international collaboration, placing Australia at the forefront of this emerging field.Read moreRead less
Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that ....Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that have identified the earliest marker of endocardial differentiation and devised the method to make endocardium from stem cells. Knowledge from this work will inform future research into growing and regenerating damaged tissue.Read moreRead less
Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family protei ....Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family proteins as specific effectors. This project will address a new paradigm in genetics that is likely to underpin development.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE130100986
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
An innovative platform using non-coding ribonucleic acids (RNAs) to control stem cell differentiation outcomes. It is difficult to control the tissue type that stem cells will form when combined with biomaterials, as the outcome is influenced by the 'stiffness' of the surface to which the stem cells attach. This project will determine how non-coding ribonucleic acids (RNAs) control stem cell behaviours and use this information to direct stem cell differentiation outcomes.
Special Research Initiatives - Grant ID: SR1101002
Funder
Australian Research Council
Funding Amount
$21,000,000.00
Summary
Stem Cells Australia. Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw upon strengths within Australia’s premier stem cell research universities and institutes. This collaboration between leading bioengineering, nanotechnology, stem cell and advanced molecular analysis experts, will fast-track efforts to deliver a fundamental understanding of the mechanisms of stem cell regulation and differentiation, and ....Stem Cells Australia. Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw upon strengths within Australia’s premier stem cell research universities and institutes. This collaboration between leading bioengineering, nanotechnology, stem cell and advanced molecular analysis experts, will fast-track efforts to deliver a fundamental understanding of the mechanisms of stem cell regulation and differentiation, and the ability to control and influence this process. Stem Cells Australia will deliver new methods for stem cell propagation and manipulation, new translational technologies for therapeutic applications, and will prepare Australia’s future stem cell scientific leaders.Read moreRead less
Failure Mechanisms of Roof Cladding under Fluctuating Wind Loads. Wind damage to low-rise buildings cause disruption to communities and result in economic losses. Improvements to the most vulnerable part (i.e. roof) will therefore have national benefits and associated economic benefits. Currently, roofing systems used in cyclonic areas are evaluated to DABM in the Northern Territory and to TR440 elsewhere, requiring the same product be tested under two different (and unsatisfactory) specificat ....Failure Mechanisms of Roof Cladding under Fluctuating Wind Loads. Wind damage to low-rise buildings cause disruption to communities and result in economic losses. Improvements to the most vulnerable part (i.e. roof) will therefore have national benefits and associated economic benefits. Currently, roofing systems used in cyclonic areas are evaluated to DABM in the Northern Territory and to TR440 elsewhere, requiring the same product be tested under two different (and unsatisfactory) specifications. This is due to the limited understanding of wind-induced fatigue of cladding. This project will provide the framework for understanding and minimizing cladding fatigue. A realistic single test will also reduce cost and result in better design systems.Read moreRead less
The response of beams subjected to axial load and lateral soil movements. Beams (piles, soil nails, and pipelines) are not only subjected to axial (vertical, axial and longitudinal)loading, but often withstand simultaneous lateral loading, due to either explicit structural loads, or due to loads induced by movement of the soils in which they are founded. Bridge piles adjacent to an approach embankment are one example. This project will provide experimental evidence to assist with the estimation ....The response of beams subjected to axial load and lateral soil movements. Beams (piles, soil nails, and pipelines) are not only subjected to axial (vertical, axial and longitudinal)loading, but often withstand simultaneous lateral loading, due to either explicit structural loads, or due to loads induced by movement of the soils in which they are founded. Bridge piles adjacent to an approach embankment are one example. This project will provide experimental evidence to assist with the estimation of capacity, axial and shear resistance, the validation of unified solutions for beams subjected to simultaneous lateral soil movements and axial loads. Such experimental evidence and analytical solutions are not currently available. The evidence will also assist with solving a contradictory, existing design principle, which will lead to an economic and efficient design approach.Read moreRead less
Harnessing properties of liquid metals for future devices. This project aims to hybridise low toxicity liquid metal alloys of gallium with surface confined functional micro/nano materials and explore fundamental new fluidic and physical-chemistry phenomena. Liquid metals are an under-used group of materials, but their combination of flexibility, bestowed by their room temperature fluidity, and metallic properties means they demonstrate startling behaviour. The expected outcomes are new devices a ....Harnessing properties of liquid metals for future devices. This project aims to hybridise low toxicity liquid metal alloys of gallium with surface confined functional micro/nano materials and explore fundamental new fluidic and physical-chemistry phenomena. Liquid metals are an under-used group of materials, but their combination of flexibility, bestowed by their room temperature fluidity, and metallic properties means they demonstrate startling behaviour. The expected outcomes are new devices and systems such as reconfigurable and highly efficient actuators/generators, catalysts, sensors, and electronic and optical components.Read moreRead less
Role of Reactive Particles in Explosive Emulsions. Concentrated water-in oil explosive emulsions are widely used in the minerals industry because they are cheap, easily detonated and relatively safe to handle. Their explosive energy can be significantly increased when reactive particles are introduced into the emulsion matrix. To do this, the interaction between the solid, oil, and water phases needs to be optimised. This investigation will increase our basic understanding of the physical and ch ....Role of Reactive Particles in Explosive Emulsions. Concentrated water-in oil explosive emulsions are widely used in the minerals industry because they are cheap, easily detonated and relatively safe to handle. Their explosive energy can be significantly increased when reactive particles are introduced into the emulsion matrix. To do this, the interaction between the solid, oil, and water phases needs to be optimised. This investigation will increase our basic understanding of the physical and chemical interactions that occur between the particle and the oil-water interface, and develop a more efficient explosive that can be produced continuously on a commercial scale.Read moreRead less