ISG60, A Novel Interferon-induced Protein: Cell Growth Inhibitory Actions
Funder
National Health and Medical Research Council
Funding Amount
$197,030.00
Summary
The interferons are signaling molecules produced by cells as part of an early warning sytsem to alert nearby tissue cells and immune cells to defend themselves against an impending viral attack or aberrant growth of cells. We have discovered ISG60, a new member of a group of proteins called the ISG54 family. The production of this family of proteins is turned on in all cells responding to the interferons. Exactly what the members of the protein family do within cells remains to be established. H ....The interferons are signaling molecules produced by cells as part of an early warning sytsem to alert nearby tissue cells and immune cells to defend themselves against an impending viral attack or aberrant growth of cells. We have discovered ISG60, a new member of a group of proteins called the ISG54 family. The production of this family of proteins is turned on in all cells responding to the interferons. Exactly what the members of the protein family do within cells remains to be established. However, by preparing cells which produce the ISG60 protein, we have found that it severely affects their growth, slowing the growth rate down and making the cells divide abnormally to become large, containing many nuclei and others dying. We propose that ISG60 binds to the structures involved in cell division and we have preliminary evidence that ISG60 interacts with an important protein involved in cell regulation, the retinoblastoma protein (pRb). The aim of this project is to more fully understand the role of ISG60 in cells. In particular, we aim to determine if ISG60 interacts with other important proteins inside cells. We shall explore the relationship of ISG60 function inside cells in greater detail as it should provide new insight into ways in which cell growth is regulated. This study will also provide insight into how the slowing of cell growth makes the cells less suitable for viral infection and reproduction, as well as providing new approaches for preventing the growth of cancer cells.Read moreRead less
Is High-school Screening For Hereditary Haemochromatosis Acceptable And Feasible?
Funder
National Health and Medical Research Council
Funding Amount
$728,573.00
Summary
Hereditary haemochromatosis (HH) is a preventable adult onset genetic iron overload disorder. In this research project we will offer senior high school students the opportunity to be tested for their risk of developing HH. Testing will be by a painless cheek brush test. Using questionnaires and interviews we will assess the psychological impact on the students. We will also assess whether those who are at increased risk of HH take steps to prevent disease.
A Healthy Promoting School Approach To Encourage Children To Wear Helmets
Funder
National Health and Medical Research Council
Funding Amount
$165,708.00
Summary
Each year around sixty Australians die as a result of bicycle crashes and thousands more are injured, some so severely they are permanently disabled. The largest proportion of the population affected by bicycle-related injuries are children and adolescents. These injuries are largely preventable if riders wore helmets. Some studies suggest wearing a helmet reduces the likelihood of head injury by more than 75%. Approximately 80% of Western Australian primary school students who ride to school we ....Each year around sixty Australians die as a result of bicycle crashes and thousands more are injured, some so severely they are permanently disabled. The largest proportion of the population affected by bicycle-related injuries are children and adolescents. These injuries are largely preventable if riders wore helmets. Some studies suggest wearing a helmet reduces the likelihood of head injury by more than 75%. Approximately 80% of Western Australian primary school students who ride to school wear a helmet. Students aged 10-12 years are on the cusp of a dramatic decline in helmet usage - secondary school students helmet usage rates are observed at 43%. This project aims to provide a supportive school environment for road users, and with the assistance of their peers, skill children with strategies and dialogue to resist peer pressure not to wear a helmet in order to arrest this rate of decline when they reach secondary school. This three-year study will compare the impact of a whole-school road safety intervention based on the health promoting school model to the standard road safety curriculum. For the study cohort ie: Year 5 - 6 students, the focus will be on bicycle safety, in particular, correct helmet usage. The intervention includes innovative participatory strategies to develop a supportive school environment for road users plus peer-teacher classroom activities that encourage the correct use of bicycle helmets for Year 5 - 6 students (and their parents). The intervention trial will be conducted with 30 Western Australian primary schools in 2000 and 2001. Other target groups of the intervention are teachers, Year 7 peer teachers, school administrators and the school community. Potential benefits of this project include the provision of a model health promoting school intervention to increase the frequency of correct helmet wearing by children which in time may lead to a reduction in bicycle-related morbidity and mortality in Western Australia.Read moreRead less
Developing And Applying Biologically Plausible Statistical Models For Normal And Non-normal Family Data
Funder
National Health and Medical Research Council
Funding Amount
$339,700.00
Summary
Although molecular and computing advances have enabled more detailed investigations of inherited diseases and the ability to fit realistic statistical models to these data, limitations still exist when analysing family data. Often only basic statistical analyses are performed, due to the lack of understanding of complexities within the data and-or inability of researchers to fit appropriate statistical models. These factors have hampered the search for genes and environmental factors influencing ....Although molecular and computing advances have enabled more detailed investigations of inherited diseases and the ability to fit realistic statistical models to these data, limitations still exist when analysing family data. Often only basic statistical analyses are performed, due to the lack of understanding of complexities within the data and-or inability of researchers to fit appropriate statistical models. These factors have hampered the search for genes and environmental factors influencing common diseases. This project aims to develop novel, biologically realistic statistical models for investigation of common, complex diseases, such as heart disease and cancer, in families. These models will incorporate both measured and unmeasured genetic and environmental factors, and will be applicable to both normally distributed and non-normally distributed traits. Model fitting will use computer-intensive simulation techniques. Application of the models to data from two large pre-existing studies of international renown, the Victorian Family Heart Study and the Australian Prostate Cancer Family Study, will enable a better understanding of the genetic and environmental factors influencing heart disease and cancer. The models will also be applicable to many other studies of diseases which use data from families, and allow more accurate and useful information to be obtained from data. Software will also be made freely available to other researchers. This will ultimately translate into better outcomes from familial genetic research, and eventually, better prevention, detection, and treatment of the diseases.Read moreRead less
Dynamics And Mechanisms Of Immune Complex-mediated Skin Inflammation
Funder
National Health and Medical Research Council
Funding Amount
$526,467.00
Summary
Type III hypersensitivity underlies a number of common autoimmune diseases, including rheumatoid arthritis and lupus erythematosus. These diseases are caused by the deposition of immune complexes (IC) and the accumulation of neutrophils within small blood vessels. We will use real time imaging to dissect in space and time the recruitment of neutrophils and IC deposition during type III hypersensitivity reactions in order to better understand the pathogenesis of these conditions.
The Role Of Non-classical MHC Class I Molecules In Adaptive Immunity
Funder
National Health and Medical Research Council
Funding Amount
$443,834.00
Summary
Specialised proteins called MHC class Ia molecules (MHC-Ia) stimulate killer T cells to lyse virus infected cells. In contrast, the function of the closely related MHC-Ib is uncertain. Recent findings have demonstrated that MHC-Ib can also be recognised by T cells and this interaction is important in the control of viral infections. However, despite the similarity to MHC-Ia, it is unclear how this interaction occurs. This project aims to investigate how killer T cells recognise MHC-Ib molecules.