Synthetic Biology Derived Electroactive Whole Cell Microbial Biosensors. The aim of this project is to develop, using synthetic biology, electrically integrated microbial biosensors for the detection of heavy metals in the environment. Building on our existing technology, this project aims to produce novel ‘biobricks’ capable of electrically integrating electric microbes into real time environmental monitors for heavy metal contaminants. This expansion of synthetic biology, and integration of el ....Synthetic Biology Derived Electroactive Whole Cell Microbial Biosensors. The aim of this project is to develop, using synthetic biology, electrically integrated microbial biosensors for the detection of heavy metals in the environment. Building on our existing technology, this project aims to produce novel ‘biobricks’ capable of electrically integrating electric microbes into real time environmental monitors for heavy metal contaminants. This expansion of synthetic biology, and integration of electric bacteria into sensor systems, will result in a new platform technology that expands our abilities to protect the ecology, agriculture and health of terrestrial, marine and agricultural at risk areas from economic and environmental damage.Read moreRead less
Microbial infestation of pre-painted steel building materials: chemical and microbial characterization, model development and control strategies. Coated steel building materials are a multi-billion dollar Australian industry. Microorganisms form slime layers on those materials, which are unsightly and reduce their energy benefits. The project will identify the problem organisms, the factors that facilitate their growth and will develop novel biofilm resistant, functional building materials.
Biogeochemical remediation approaches for PFAS contaminated environments. This project aims to identify and harvest microorganisms capable of directly or indirectly affecting PFOS or PFOA degradation in the environment. Fluorinated compounds such as PFOS and PFOA in firefighting foams are contaminants of concern now routinely detected in contaminated groundwater and soil globally. Understanding the role of microorganisms, and the biogeochemical processes they perform in relation to fluorinated c ....Biogeochemical remediation approaches for PFAS contaminated environments. This project aims to identify and harvest microorganisms capable of directly or indirectly affecting PFOS or PFOA degradation in the environment. Fluorinated compounds such as PFOS and PFOA in firefighting foams are contaminants of concern now routinely detected in contaminated groundwater and soil globally. Understanding the role of microorganisms, and the biogeochemical processes they perform in relation to fluorinated compounds, will inform handling of contaminated sites and lead to development of cost effective and sustainable remediation technologies. Read moreRead less
The toxins of water-borne cyanobacteria: regulation and exploitation of their biosynthesis. Water quality is a major concern in Australia, as is the global need for new natural products with antibiotic activity. The mechanisms by which cyanobacteria produce toxins that reduce the quality of water may very well be the answer to the lack of novel medicinal compounds currently being discovered in nature. Encompassed in this one program are the aims of ameliorating the effects of toxic algal blooms ....The toxins of water-borne cyanobacteria: regulation and exploitation of their biosynthesis. Water quality is a major concern in Australia, as is the global need for new natural products with antibiotic activity. The mechanisms by which cyanobacteria produce toxins that reduce the quality of water may very well be the answer to the lack of novel medicinal compounds currently being discovered in nature. Encompassed in this one program are the aims of ameliorating the effects of toxic algal blooms as well as introducing the means for the design and synthesis of a range of novel bioactive products. The benefits include better water quality and biosafety management options, a new generation of drug design and discovery, and the associated transformation of environmental and medical research and education in Australia.Read moreRead less
Function and application of novel proteins from sponge symbionts. This project aims to determine the function of eukaryotic-like proteins (ELPs) from bacterial symbionts of sponges and apply this knowledge to develop new tools for biotechnology. This project will use innovative microscopy techniques and gene expression studies to define the molecular and cellular interactions of ELPs with sponges and how this is influenced by changing environmental conditions. ELPs will be further used to create ....Function and application of novel proteins from sponge symbionts. This project aims to determine the function of eukaryotic-like proteins (ELPs) from bacterial symbionts of sponges and apply this knowledge to develop new tools for biotechnology. This project will use innovative microscopy techniques and gene expression studies to define the molecular and cellular interactions of ELPs with sponges and how this is influenced by changing environmental conditions. ELPs will be further used to create new, artificial interactions between bacteria and eukaryotes. This project will provide fundamental knowledge on the evolution and function of newly discovered ELPs found in both beneficial and pathogenic bacteria and paves the way to control symbiosis for biotechnological applications.Read moreRead less
In situ microbial conversion of coal to methane: Biotechnology development for clean use of Australian coal. We will develop a biotechnology that uses native microorganisms to accelerate the underground conversion of coal to methane. Approximately 90% of Australia’s coal resources cannot be accessed economically using traditional mining technologies. A technology that converts coal to methane could generate an energy supply worth an estimated $60 billion, foster the development of an energy indu ....In situ microbial conversion of coal to methane: Biotechnology development for clean use of Australian coal. We will develop a biotechnology that uses native microorganisms to accelerate the underground conversion of coal to methane. Approximately 90% of Australia’s coal resources cannot be accessed economically using traditional mining technologies. A technology that converts coal to methane could generate an energy supply worth an estimated $60 billion, foster the development of an energy industry now in its infancy, and generate numerous new employment opportunities. Environmentally, methane is a cleaner burning fuel than coal, uses much less water for processing and generates the same quantity of electricity with lower CO2 emissions. This project highlights the fact that Australia’s microbial diversity is a resource we cannot afford to ignore.Read moreRead less
A novel method for controlling microbial concrete corrosion in sewers. This project plans to use a newly discovered, low-cost and environmental benign antimicrobial agent to develop an innovative technology to control the development of corrosion-inducing sewer biofilms. Concrete sewer corrosion is a long-standing and costly problem for the water industry. Microbial hydrogen sulfide oxidation on concrete surfaces plays a critical role. The technology will be designed to prevent corrosion of new ....A novel method for controlling microbial concrete corrosion in sewers. This project plans to use a newly discovered, low-cost and environmental benign antimicrobial agent to develop an innovative technology to control the development of corrosion-inducing sewer biofilms. Concrete sewer corrosion is a long-standing and costly problem for the water industry. Microbial hydrogen sulfide oxidation on concrete surfaces plays a critical role. The technology will be designed to prevent corrosion of new concrete sewers by adding a precursor chemical into the cement, or to slow down the corrosion of existing sewers by infrequently (once every one to few years) spraying the precursor chemical directly onto the concrete surface. Potentially, the project will substantially reduce sewer corrosion.Read moreRead less
Exploring and harnessing mobile DNA: Integrons and gene cassettes in natural populations of Bacteria. Bacteria respond rapidly to environmental change by acquiring new genes via lateral gene transfer. The integron/gene cassette system is important in this process as it is found in an increasingly broad range of bacteria. As well as being common, we have shown that the system is associated with an unprecedented amount of genetic novelty. Here we explore the limits of this novelty and its con ....Exploring and harnessing mobile DNA: Integrons and gene cassettes in natural populations of Bacteria. Bacteria respond rapidly to environmental change by acquiring new genes via lateral gene transfer. The integron/gene cassette system is important in this process as it is found in an increasingly broad range of bacteria. As well as being common, we have shown that the system is associated with an unprecedented amount of genetic novelty. Here we explore the limits of this novelty and its contribution to bacterial evolution. In so doing we have the potential to identify new commercially important genes and develop enabling technologies. These discoveries could produce beneficial outcomes for exploitation by a wide range of Australian industries.Read moreRead less
Enhanced algal biofuel production: optimising photosynthesis in Australian strains of marine algae. Algal biofuel produces a sustainable liquid fuel to help meet our future energy needs. This project will pioneer a multifaceted approach in molecular biology and photophysiology to engineer the best biofuel producers from Australian marine algae and will advance innovation in Australia's biofuel biotechnology development.
Discovery Early Career Researcher Award - Grant ID: DE160100615
Funder
Australian Research Council
Funding Amount
$348,200.00
Summary
Harnessing chain-forming diatoms for improved lipid biofuel production. The aim of this project is to unlock the molecular secrets of highly productive chain-forming diatom microalgae that allow them to produce high levels of biofuel lipids. The formation of multicellular chains appears key to the success of some of the most widespread and productive diatom species. Through a combination of systems biology, bioinformatics, and genetics experiments, this project aims to investigate the relationsh ....Harnessing chain-forming diatoms for improved lipid biofuel production. The aim of this project is to unlock the molecular secrets of highly productive chain-forming diatom microalgae that allow them to produce high levels of biofuel lipids. The formation of multicellular chains appears key to the success of some of the most widespread and productive diatom species. Through a combination of systems biology, bioinformatics, and genetics experiments, this project aims to investigate the relationship between chain formation and biofuel lipid productivity in Chaetoceros diatoms, and to discover genes and molecules that encode and influence these traits. The knowledge and technology generated as a result may improve biofuel yields, increase the robustness of species growing in open pond systems, and reduce processing costs such as de-watering.Read moreRead less