Pre-blast screening of improvised explosive devices - a National counter-terrorism initiative. The proposed research is focused on the specific needs of Australian counter-terrorism interests, including those of border protection, the customs service, transport authorities, forensic laboratories, etc. The support of this proposal will ensure that Australia, and its States and Territories are protected against terrorist threats. The support provided by the collaborating organisations from the var ....Pre-blast screening of improvised explosive devices - a National counter-terrorism initiative. The proposed research is focused on the specific needs of Australian counter-terrorism interests, including those of border protection, the customs service, transport authorities, forensic laboratories, etc. The support of this proposal will ensure that Australia, and its States and Territories are protected against terrorist threats. The support provided by the collaborating organisations from the various Federal and State police and forensic agencies, and the customs service, etc, highlights the importance of this project to the nation. Finally, a PhD student and a research assistant will be involved with the project and will gain specialised skills positioning them to make strong contributions to Australia's counter-terrorism measures.Read moreRead less
Green Manufacturing of Graphene from Indigenous Natural Graphite and Graphene-based Nanofiltration Membranes. This project aims to investigate radical new approaches to reduce chemical and energy requirements for transformation of indigenous natural graphite to a high-value material graphene. The graphite which will be used is the by-product of the Uley mines of South Australia. Graphene is an atomically thin arrangement of carbon atoms with combinations of remarkable chemical inertness, strengt ....Green Manufacturing of Graphene from Indigenous Natural Graphite and Graphene-based Nanofiltration Membranes. This project aims to investigate radical new approaches to reduce chemical and energy requirements for transformation of indigenous natural graphite to a high-value material graphene. The graphite which will be used is the by-product of the Uley mines of South Australia. Graphene is an atomically thin arrangement of carbon atoms with combinations of remarkable chemical inertness, strength, and massive surface area. Utilising fluid phase dispersed graphene, this project aims to develop scalable and industrially-adaptable methods to manufacture thin yet mechanically robust, inert, fouling-resistant, highly-permeable graphene-based asymmetric membranes. These advanced membranes are expected to find wide application in reducing discharge of mining effluents and recovery of precious metals.Read moreRead less
On-site and comprehensive technology for chemical weapons, toxins and drugs. This project aims to evaluate and validate broad capabilities of advanced chemical profiling using benchtop and portable gas chromatography–mass spectrometry for forensic applications. Establishing guidelines for sampling various matrices, key performance measures, and improving chemical and residue identification for drug profiling in clandestine laboratories and public venues, chemical warfare agents, and chemical tox ....On-site and comprehensive technology for chemical weapons, toxins and drugs. This project aims to evaluate and validate broad capabilities of advanced chemical profiling using benchtop and portable gas chromatography–mass spectrometry for forensic applications. Establishing guidelines for sampling various matrices, key performance measures, and improving chemical and residue identification for drug profiling in clandestine laboratories and public venues, chemical warfare agents, and chemical toxins is expected. Anticipated outcomes for project partners in defence and policing include detailed knowledge of sample and residue composition and on-site assessment of chemical risks with immediate feedback. Benefits should include improved community and security services safety by enhanced detection of harmful substances.Read moreRead less
Oxidation product generation during heating and storage of bio-fuels and alternative fuels assessed by multidimensional gas chromatography. Renewable bio-fuels offer much promise as a replacement for fossil fuels. However, they have a tendency to oxidise when prepared and used, potentially forming gums and solid deposits in engines. The objective of this project is the identification of these deposits and subsequent prevention during engine operation.
Tailoring cellulose properties by manipulating cellulose synthase. Cellulose, a highly abundant polymer produced by plants, has many existing uses in Australian fibre and polymer industries and potential uses as, for example, an abundant feedstuff for biomass conversion into ethanol and other high value products. The optimal properties for different applications vary so that, for example, high crystallinity cellulose gives strong fibres whereas low crystallinity cellulose dissolves in gentler so ....Tailoring cellulose properties by manipulating cellulose synthase. Cellulose, a highly abundant polymer produced by plants, has many existing uses in Australian fibre and polymer industries and potential uses as, for example, an abundant feedstuff for biomass conversion into ethanol and other high value products. The optimal properties for different applications vary so that, for example, high crystallinity cellulose gives strong fibres whereas low crystallinity cellulose dissolves in gentler solvents on the way to producing cellulose-based polymers. By exploring ways to adjust the properties of celluloses for use in different applications, we can deliver potential benefits to primary producers, industries and the environment.
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Discovery of new genes for plant cellulose biosynthesis and improved fibre production. Cellulose, the world's most abundant biopolymer, is important to the cotton and forest industries and for human and animal nutrition. Before biotechnology can manipulate cellulose, we must identify the enzymes of the synthesis pathway and understand how their properties determine the properties of the cellulose they produce. Not all enzymes are known and any relationships to cellulose properties remain unexplo ....Discovery of new genes for plant cellulose biosynthesis and improved fibre production. Cellulose, the world's most abundant biopolymer, is important to the cotton and forest industries and for human and animal nutrition. Before biotechnology can manipulate cellulose, we must identify the enzymes of the synthesis pathway and understand how their properties determine the properties of the cellulose they produce. Not all enzymes are known and any relationships to cellulose properties remain unexplored. This study extends our successful mutational analysis of cellulose synthesis in Arabidopsis and initiates the molecular analysis of organisms making cellulose with distinctive properties. It will significantly advance knowledge of cellulose biosynthesis and identify novel genes for fibre improvement.Read moreRead less
Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed prod ....Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed production is not currently available. In wheat alone, apomixis presents for Australia an economic value of more than Aus$ ½ billion per annum. Furthermore, controlled apomixis will accelerate breeding programs that will bring drought resistance and minimal fertiliser requiring varieties to the farmer.Read moreRead less
Control of meiosis and embryogenesis as a means to induce higher plants to reproduce asexually through seed. New plant meiosis-control genes will be isolated and characterised. These will be used, together with cell proliferation control genes characterised in the applicant's laboratory, to control gamete formation and embryo development in higher plants, and hence plant reproduction. This research will provide a platform for genetic fixation of hybrid vigour and repeat propagation of F1 hybrid ....Control of meiosis and embryogenesis as a means to induce higher plants to reproduce asexually through seed. New plant meiosis-control genes will be isolated and characterised. These will be used, together with cell proliferation control genes characterised in the applicant's laboratory, to control gamete formation and embryo development in higher plants, and hence plant reproduction. This research will provide a platform for genetic fixation of hybrid vigour and repeat propagation of F1 hybrid seed without attenuation of vigour. The technology will increase yield and profitability for Australian agriculture, and ensure access to technology that the industry partner will otherwise develop overseas. The technology benefits most major crops that are bred and grown to meet increasing demand for food and speciality products for pharmaceutical or industrial purposes.Read moreRead less
The role of the Ttyh1 protein in cell activation. We have cloned TTYH1, a human homologue of the Drosophila melanogaster tweety gene. The mouse gene has also been identified. The predicted structure of the protein is a membrane protein with 5 transmembrane domains. We have also expressed a GFP-tagged fusion protein in mouse fibroblasts. Confocal microscopy indicates that this protein is likely to be a novel adhesion molecule, with a cellular distribution characteristic of molecules such as integ ....The role of the Ttyh1 protein in cell activation. We have cloned TTYH1, a human homologue of the Drosophila melanogaster tweety gene. The mouse gene has also been identified. The predicted structure of the protein is a membrane protein with 5 transmembrane domains. We have also expressed a GFP-tagged fusion protein in mouse fibroblasts. Confocal microscopy indicates that this protein is likely to be a novel adhesion molecule, with a cellular distribution characteristic of molecules such as integrins. We aim to determine the function of Ttyh1, its interacting intra- and extra-cellular proteins and to assess its candidature as a molecule of importance in cell migration and adhesion.Read moreRead less
Why is the photosynthetic CO2-fixing enzyme, Rubisco, so inefficient? Dissection of the catalytic chemistry by computational simulation and experimental testing. Fixation of CO2 by the enzyme Rubisco during photosynthesis produces organic compounds which feed all life. Despite this critical role, Rubisco catalyses its reaction sluggishly and, worse, discriminates poorly between CO2 and O2, leading to useless products. Our combined expertise equips us to analyse Rubisco's mechanism using quantum- ....Why is the photosynthetic CO2-fixing enzyme, Rubisco, so inefficient? Dissection of the catalytic chemistry by computational simulation and experimental testing. Fixation of CO2 by the enzyme Rubisco during photosynthesis produces organic compounds which feed all life. Despite this critical role, Rubisco catalyses its reaction sluggishly and, worse, discriminates poorly between CO2 and O2, leading to useless products. Our combined expertise equips us to analyse Rubisco's mechanism using quantum-chemical methods and then test predictions experimentally. We will capitalise on our previous successful studies of Rubisco by addressing emergent issues which are the keys to understanding catalytic efficiency and CO2/O2 selectivity: the roles of a carbamylated lysine; the way CO2 addition is rendered irreversible; and the spin inversion inherent in O2 addition.Read moreRead less