Structure and dynamics of a multiprotein-mRNA complex involved in the regulation of gene expression. RNA/protein interactions are now recognised as a major control point in the regulation of gene-expression. Proteins such as HuR and the poly(C)-binding proteins (PCBPs) act to stabilise and transport specific messenger (m)RNAs, and thus determine their translation levels. In contrast to such an important function, very little is known about these protein/mRNA interactions at an atomic level. The ....Structure and dynamics of a multiprotein-mRNA complex involved in the regulation of gene expression. RNA/protein interactions are now recognised as a major control point in the regulation of gene-expression. Proteins such as HuR and the poly(C)-binding proteins (PCBPs) act to stabilise and transport specific messenger (m)RNAs, and thus determine their translation levels. In contrast to such an important function, very little is known about these protein/mRNA interactions at an atomic level. The current study will investigate the structural and biophysical properties of a recently discovered HuR/PCBP/mRNA complex implicated in the regulation of androgen receptor expression. This information has the potential to assist in the development of drugs to reduce AR expression in prostate cancer.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100148
Funder
Australian Research Council
Funding Amount
$150,916.00
Summary
An STM/AFM Facility for Electroactive Materials Characterisation. A Scanning Tunnelling Microscope (STM)/Atomic Force Microscope (AFM) facility for electroactive materials characterisation: This project is expected to address an identified need for the characterisation of electroactive structures using scanning probe microscopy and builds on local expertise in allied methods. The instrumentation includes an electrochemical STM for electrical testing of molecular wires, switches, transistors and ....An STM/AFM Facility for Electroactive Materials Characterisation. A Scanning Tunnelling Microscope (STM)/Atomic Force Microscope (AFM) facility for electroactive materials characterisation: This project is expected to address an identified need for the characterisation of electroactive structures using scanning probe microscopy and builds on local expertise in allied methods. The instrumentation includes an electrochemical STM for electrical testing of molecular wires, switches, transistors and other single molecule electronic components, together with a pico-force tunnelling AFM (PF-TUNA) for the measurement and correlation of nano mechanical and electrical properties of fragile structures over larger areas. The facility will be a core asset for researchers that use electroactive material on conducting substrates in fields including fundamental corrosion science, nanotechnology, and moltronics.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0560712
Funder
Australian Research Council
Funding Amount
$630,837.00
Summary
State-of-the-art biophysical tools for the characterisation of molecular interactions. In the current era of 'the genome' there is more demand than ever before for the characterisation of the gene products - the oligonucleotides and proteins that carry out the important functions in the cell. The current proposal is to acquire a suite of instrumentation to characterise the kinetics, thermodynamics and overall affinities of interactions between biological macromolecules and their physiological bi ....State-of-the-art biophysical tools for the characterisation of molecular interactions. In the current era of 'the genome' there is more demand than ever before for the characterisation of the gene products - the oligonucleotides and proteins that carry out the important functions in the cell. The current proposal is to acquire a suite of instrumentation to characterise the kinetics, thermodynamics and overall affinities of interactions between biological macromolecules and their physiological binding partners or with novel ligands. This will reveal the role of the macromolecules of interest in the cell, and potentially lead to the discovery of drug molecules which could interfere with molecular interactions underlying disease.Read moreRead less
Calcification of acrylic hydrogels in abiotic media: mechanism and control. Poly(2-hydroxyethyl methacrylate (PHEMA) and other acrylic hydrogels are extensively used as biomaterials, yet conclusive evidence exists that they have a propensity to calcify following implantation. This process has undesirable consequences on the functionality of various prostheses. Based on preliminary observations that PHEMA can promote the deposition of calcium minerals from media devoid of biological factors, whic ....Calcification of acrylic hydrogels in abiotic media: mechanism and control. Poly(2-hydroxyethyl methacrylate (PHEMA) and other acrylic hydrogels are extensively used as biomaterials, yet conclusive evidence exists that they have a propensity to calcify following implantation. This process has undesirable consequences on the functionality of various prostheses. Based on preliminary observations that PHEMA can promote the deposition of calcium minerals from media devoid of biological factors, which appears thus to be an inherent property of the polymer, the project aims at formulating new hypotheses to explain this phenomenon, and to confirm them experimentally. The "chelation" hypothesis will be validated by modifying the structure of polymers, and the "spontaneous precipitation" hypothesis by assessing the effect of solutes on the equilibrium water content of polymers. NMR and FTIR spectrometric techniques will be used to gain further insight into the mechanism of calcification. Methods to prevent the calcification will potentially result from these experiments, however, anticalcification agents will also be incorporated into hydrogels and their effect evaluated in calcification assays.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0346892
Funder
Australian Research Council
Funding Amount
$689,000.00
Summary
Protein Over-Expression/Purification and Macromolecular Structure Determination by X-Ray Diffraction. This proposal seeks funds for state-of-the-art facilities for protein over-expression and macromolecular X-ray diffraction. This will build upon recent initiatives within the collaborating institutions in the field of Structural Biology. It will enable research groups in Perth to pursue the large-scale production of important proteins and to conduct high-resolution structural studies using X-ray ....Protein Over-Expression/Purification and Macromolecular Structure Determination by X-Ray Diffraction. This proposal seeks funds for state-of-the-art facilities for protein over-expression and macromolecular X-ray diffraction. This will build upon recent initiatives within the collaborating institutions in the field of Structural Biology. It will enable research groups in Perth to pursue the large-scale production of important proteins and to conduct high-resolution structural studies using X-ray crystallographic techniques. This technology, which is one of the most important tools in modern biology, provides unique insights into the chemical mechanisms of biological macromolecules and will significantly enhance a great breadth of biological research in Western Australia.Read moreRead less
Interaction between consolidation and lubrication of biological joints. This project aims to develop a computational model to be used in conjunction with experimental studies to understand complex lubrication systems in biological joints. Nature has equipped biological joints with a remarkable ability to achieve ultralow friction even at relatively high contact force, however the mechanisms used remain uncertain. This project intends to provide a deeper, fundamental understanding of the friction ....Interaction between consolidation and lubrication of biological joints. This project aims to develop a computational model to be used in conjunction with experimental studies to understand complex lubrication systems in biological joints. Nature has equipped biological joints with a remarkable ability to achieve ultralow friction even at relatively high contact force, however the mechanisms used remain uncertain. This project intends to provide a deeper, fundamental understanding of the friction and contact mechanisms occurring in biological joints. The project outcomes could lead to bioinspired innovation in future engineering design and advancements in materials science that have the potential to significantly benefit Australian society.Read moreRead less
Development of Canonical Mist Filter Models. Over one million tonnes of oil (mist) is wasted every year – and emitted to the atmosphere through inefficient filtration. Over 50 per cent of energy usage in most process industries is for filtration and separation processes, yet mist filters and separators are largely designed by trial and error, resulting in sub-optimal, inefficient designs. Recent advances by the research team have, only now, made it possible to develop accurate models for such sy ....Development of Canonical Mist Filter Models. Over one million tonnes of oil (mist) is wasted every year – and emitted to the atmosphere through inefficient filtration. Over 50 per cent of energy usage in most process industries is for filtration and separation processes, yet mist filters and separators are largely designed by trial and error, resulting in sub-optimal, inefficient designs. Recent advances by the research team have, only now, made it possible to develop accurate models for such systems. This work intends to be the first to develop accurate, broadly applicable models for all processes in mist filters, thereby providing immense process efficiency benefits, together with improved worker and environmental protection, and less wastage of dwindling oil resources.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE170100087
Funder
Australian Research Council
Funding Amount
$1,100,000.00
Summary
Plasma-focused ion beam for nanoscale characterisation of materials. This project aims to enable research programmes in functional materials to characterise materials using xenon-plasma focused ion beam (FIB) instrumentation. The plasma FIB, with its fast milling speeds across large areas, will enable new three-dimensional imaging experiments and types of transmission electron microscopy samples. This will have applications in engineering, photovoltaics and environmental geosciences, which all n ....Plasma-focused ion beam for nanoscale characterisation of materials. This project aims to enable research programmes in functional materials to characterise materials using xenon-plasma focused ion beam (FIB) instrumentation. The plasma FIB, with its fast milling speeds across large areas, will enable new three-dimensional imaging experiments and types of transmission electron microscopy samples. This will have applications in engineering, photovoltaics and environmental geosciences, which all need to analyse materials on a nanometre scale.Read moreRead less