Chromosomes are structures that carry genes in all our cells. Every human cell has 46 chromosomes. In the nucleus of eukaryotic cells, DNA is highly folded and compacted with specific proteins into a dynamic polymer called chromatin. Gene expression, chromosome division, DNA replication, and repair all act, not on DNA alone, but on this chromatin template. The discovery that enzymes can (re)organise chromatin into accessible and inaccessible configurations revealed mechanisms that considerably e ....Chromosomes are structures that carry genes in all our cells. Every human cell has 46 chromosomes. In the nucleus of eukaryotic cells, DNA is highly folded and compacted with specific proteins into a dynamic polymer called chromatin. Gene expression, chromosome division, DNA replication, and repair all act, not on DNA alone, but on this chromatin template. The discovery that enzymes can (re)organise chromatin into accessible and inaccessible configurations revealed mechanisms that considerably extend the information potential of the genetic code. In addition, it is now established that chromatin structural features can influence gene expression. In vitro studies support a model in which chromatin functions as a barrier for the access to DNA. Therefore this organization has to be tighly regulated and dynamic to allow the protein-DNA interactions critical for nuclear functions. Importantly genome organisation provides in addition to genetic information another layer of information, so called epigenetic, which by definition means that it is stably inherited throughout cellular divisions, yet it is not encoded genetically. Thus each cell type will display a specific epigenome. We have recently constructed small human minichromosomes, which are much easier to study than the much larger normal chromosomes. The present project proposes to define the epigenetic feature across an entire human chromosome using our minichhromosomes as working models. The outcome will be a significant gain in our knowledge on the processes underlying epigenetic regulation, the organisation of specialised chromatin domain, and behaviour of the chromosomes.Read moreRead less
Significance Of Low-level Mosaicism To Intellectual Disability In Paediatric Disorders
Funder
National Health and Medical Research Council
Funding Amount
$483,402.00
Summary
My vision for the next 4 years is to improve outcomes for children and their families with inherited disorders associated with intellectual disability (ID) and autism through earlier diagnosis and intervention. This is of great importance with annual costs of ID close $14.72 billion to the Australian health system, and missed or delayed diagnoses being a significant problem, as ID is found in 1.7% of births, where a specific cause is currently identified in less than half.
Assessment of the new technologies to maximise the internal energy efficiency by modelling the energy flows in Victorian power stations. Increased internal energy efficiency will reduce the CO2 emissions and increase the profit margin for the power stations. The aim of the proposed research is to investigate the internal energy efficiency in the brown coal fired power stations by modelling the internal energy flows to reveal the potentials in the 'waste' heat streams in the power stations, and t ....Assessment of the new technologies to maximise the internal energy efficiency by modelling the energy flows in Victorian power stations. Increased internal energy efficiency will reduce the CO2 emissions and increase the profit margin for the power stations. The aim of the proposed research is to investigate the internal energy efficiency in the brown coal fired power stations by modelling the internal energy flows to reveal the potentials in the 'waste' heat streams in the power stations, and to assess new waste heat recovery measures for the power industry. The proposed research, supported by the entire Victorian power generation industry, will help the industry to achieve their green house gas emission reduction target set by the Federal Government.
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Horizontal convection at ocean-relevant proportions. This project aims to determine the role of buoyancy differences from solar radiation and vertical confinement. Flows created by lateral variation in heat or buoyancy are poorly understood when the horizontal length greatly exceeds the height, precisely the conditions relevant to industry and understanding the role of horizontal convection in Earth's oceans and in turn the delicate current system that maintains Earth's temperate climate. This p ....Horizontal convection at ocean-relevant proportions. This project aims to determine the role of buoyancy differences from solar radiation and vertical confinement. Flows created by lateral variation in heat or buoyancy are poorly understood when the horizontal length greatly exceeds the height, precisely the conditions relevant to industry and understanding the role of horizontal convection in Earth's oceans and in turn the delicate current system that maintains Earth's temperate climate. This project proposes computational and experimental efforts to probe the ultimate regime of heat transport in very shallow horizontal convection, benefiting humankind through improvements to future ocean and climate modelling efforts.Read moreRead less
The elusive upper bound of heat transfer in horizontal convection. The question as to whether lateral variation in global ocean buoyancy resulting from low solar radiation near the poles and higher temperatures in the tropical regions (known as horizontal convection) is a driver for global ocean currents is critical to our understanding of this complex and delicate system that maintains Earth's temperate climate. This question hinges on the relationship between heat transport and strength of buo ....The elusive upper bound of heat transfer in horizontal convection. The question as to whether lateral variation in global ocean buoyancy resulting from low solar radiation near the poles and higher temperatures in the tropical regions (known as horizontal convection) is a driver for global ocean currents is critical to our understanding of this complex and delicate system that maintains Earth's temperate climate. This question hinges on the relationship between heat transport and strength of buoyancy forcing towards global scales; this project proposes a landmark experimental effort supported by detailed simulations to probe the ultimate regime of heat transport in horizontal convection. The project is expected to inform the direction of future ocean and climate modelling efforts to the benefit of humanity.Read moreRead less
Simulating two-phase electrodynamic flows in droplet-based microfluidic circuit elements. The knowledge, data and analysis tools developed within this project will facilitate the economical production of electrodynamically controlled integrated droplet-based microfluidic devices for critical high-demand applications such as: genome sequencing; protein evolution, synthesis and crystallisation; micro-structured pharmaceuticals; disposable devices for biomedical analysis; portable point-of-entry (b ....Simulating two-phase electrodynamic flows in droplet-based microfluidic circuit elements. The knowledge, data and analysis tools developed within this project will facilitate the economical production of electrodynamically controlled integrated droplet-based microfluidic devices for critical high-demand applications such as: genome sequencing; protein evolution, synthesis and crystallisation; micro-structured pharmaceuticals; disposable devices for biomedical analysis; portable point-of-entry (biochem)security analysis devices. Hence this project is an investment in enabling technologies to benefit Australia's growing biotech, pharmaceutical and micro/nanotechnology sectors. Tangible community benefits (e.g., in improved diagnostic technologies, pharmaceuticals) will result.Read moreRead less
Process scalability and performance prediction of Viscous Transfer Micro Turbines for use as miniaturised power supplies. This project aims to predict the performance and scalability of viscous transfer turbines. The work will be carried out with Micromachines Ltd., a firm developing working turbines with 50 mm rotors. The goal of the research is to gain an understanding of the scaling laws for these turbines, in order to predict their performance. Viscous transfer turbines have a very simple di ....Process scalability and performance prediction of Viscous Transfer Micro Turbines for use as miniaturised power supplies. This project aims to predict the performance and scalability of viscous transfer turbines. The work will be carried out with Micromachines Ltd., a firm developing working turbines with 50 mm rotors. The goal of the research is to gain an understanding of the scaling laws for these turbines, in order to predict their performance. Viscous transfer turbines have a very simple disc construction making them ideal for manufacturing as small-scale units (with a 5 mm rotor). Small enough to replace batteries, but with a higher power output, they would provide the need for higher power output devices to replace batteries.Read moreRead less
Gravity Current Driven Smoke Dispersion In a Stratified Ambient. Smoke from bushfires transported by gravity currents, and known to occur nationwide, caused the shutdown of businesses, education and events in Canberra in 2019. Recent scientific investigations have shown that the speed of propagation and concentration of smoke in these three-dimensional gravity currents have a long term ‘memory’ of their initial configuration. In this project, high-fidelity computational and experimental techniq ....Gravity Current Driven Smoke Dispersion In a Stratified Ambient. Smoke from bushfires transported by gravity currents, and known to occur nationwide, caused the shutdown of businesses, education and events in Canberra in 2019. Recent scientific investigations have shown that the speed of propagation and concentration of smoke in these three-dimensional gravity currents have a long term ‘memory’ of their initial configuration. In this project, high-fidelity computational and experimental techniques will be used to elucidate the fundamental mechanisms of gravity current entrainment and propagation. This knowledge will set a strong foundation to improve operational forecasts of smoke transport that will allow government agencies to better respond to the negative impact of these complicated flows.Read moreRead less
DROP DEFORMATION IN CONFINED MICROFLUIDIC GEOMETRIES. Increasingly, high technology applications in biotechnology and microtechnology industries need to process complex (non-Newtonian) fluids with dispersed particles/droplets in channels as small as several microns (microfluidics). A computational fluid dynamic model of non-Newtonian droplet deformation in microfluidic geometries will be developed, and validated using experimental measurements of the flow field in this project. The aim is to und ....DROP DEFORMATION IN CONFINED MICROFLUIDIC GEOMETRIES. Increasingly, high technology applications in biotechnology and microtechnology industries need to process complex (non-Newtonian) fluids with dispersed particles/droplets in channels as small as several microns (microfluidics). A computational fluid dynamic model of non-Newtonian droplet deformation in microfluidic geometries will be developed, and validated using experimental measurements of the flow field in this project. The aim is to understand and quantify factors influencing droplet deformation. Coupling non-Newtonian characteristics with microfluidic geometries will allow the continuous manufacture of micro-particles of specified size and shape for existing and new applications, and will provide guidance for further extending the process to nano-particle manufacture.Read moreRead less