Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through ....Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through helping ensure the profitability of vital Australian export agricultural industries and the sustainability of the soil/land resource. This proposal will ensure that Australia leads international herbicide resistance research and will enable the capture of intellectual property and commercial opportunities. Read moreRead less
Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Toba ....Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Tobacco yellow dwarf mastrevirus. In this project, we aim to further develop this strategy into a broad and stable mechanism for generating resistance to all known geminiviruses and nanoviruses.Read moreRead less
A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants w ....A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants with nematodes. It is based on an Australian patent which has wide applicability in plant biotechnology. Nematode control will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Putting smells into context: Using in vivo technologies to understand plant-insect odour signalling. How insects find host plants is central to understanding their basic ecology and management. We use a novel cross-disciplinary approach to target a key pest insect's responses to a host-plant, genetically modified to smell different. This insect pest, the cotton bollworm, costs the Australian economy millions in lost revenue every year. Reliance on insecticides to control insect pests is exacerba ....Putting smells into context: Using in vivo technologies to understand plant-insect odour signalling. How insects find host plants is central to understanding their basic ecology and management. We use a novel cross-disciplinary approach to target a key pest insect's responses to a host-plant, genetically modified to smell different. This insect pest, the cotton bollworm, costs the Australian economy millions in lost revenue every year. Reliance on insecticides to control insect pests is exacerbated by increasing insecticidal resistance and is a major economic, environmental and health concern. Our research will open the door to novel control strategies that use the natural odours of plants to reduce their attractiveness to insect pests.Read moreRead less
Seed quality and disease resistance trait mapping in lentil (Lens culinaris ssp. culinaris). To protect and increase our world lentil market share, the mission of the Australian lentil breeders is to develop lentil varieties with superior seed qualities. Three highly desirable seed quality traits are; fatter and larger shape, better splitting efficiency and resistance to blemishing by ascochyta blight infection. To speed up the development of varieties with these traits, the genetic loci governi ....Seed quality and disease resistance trait mapping in lentil (Lens culinaris ssp. culinaris). To protect and increase our world lentil market share, the mission of the Australian lentil breeders is to develop lentil varieties with superior seed qualities. Three highly desirable seed quality traits are; fatter and larger shape, better splitting efficiency and resistance to blemishing by ascochyta blight infection. To speed up the development of varieties with these traits, the genetic loci governing them will be characterised and closely associated molecular markers identified. Markers will be validated for their use in marker-assisted breeding programs via back-crossing and field and glass house testing.Read moreRead less
Pyramiding ascochyta blight (A. rabiei) resistance in chickpea. Ascochyta blight (A. rabiei) is the most devastating disease that affects chickpea production in Australia. This project will produce enhanced chickpea breeding germplasm with sustainable resistance to ascochyta blight. This will be achieved through the enrichment of highly informative genome linkage maps and the development of molecular markers closely associated with novel resistance genes. Markers will be used to screen backcross ....Pyramiding ascochyta blight (A. rabiei) resistance in chickpea. Ascochyta blight (A. rabiei) is the most devastating disease that affects chickpea production in Australia. This project will produce enhanced chickpea breeding germplasm with sustainable resistance to ascochyta blight. This will be achieved through the enrichment of highly informative genome linkage maps and the development of molecular markers closely associated with novel resistance genes. Markers will be used to screen backcrossed populations for novel resistance in order to pyramid the genes involved. The most resistant lines, to all of the A. rabiei pathotypes, will be selected for use in future resistance breeding programs.Read moreRead less
Identifying potential barriers to transplanting modified forms of the CO2-fixing enzyme, Rubisco, into plants. Improving the ability of crops to use water, light and fertiliser more efficiently would have economic benefits and ease the environmental impacts associated with agricultural practices. It is thought that such improvements can be made by enhancing the efficiency of the photosynthetic protein, Rubisco, which fixes most of the CO2 in the biosphere. The research proposed here uses unique ....Identifying potential barriers to transplanting modified forms of the CO2-fixing enzyme, Rubisco, into plants. Improving the ability of crops to use water, light and fertiliser more efficiently would have economic benefits and ease the environmental impacts associated with agricultural practices. It is thought that such improvements can be made by enhancing the efficiency of the photosynthetic protein, Rubisco, which fixes most of the CO2 in the biosphere. The research proposed here uses unique Rubisco transplantation capabilities that I have developed to improve our fundamental understanding of how Rubisco is processed and its activity regulated in plants. This will pave the way for our ongoing efforts to engineer and transplant more efficient Rubisco into crops.Read moreRead less
Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a nov ....Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a novel NUE technology that when transferred to plants significantly improves NUE. We will define the phenotype at the molecular, biochemical and physiological levels to maximise its adoption to other agricultural crops such as wheat, barley and maize.Read moreRead less
Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-valu ....Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-value alternative products. In addition, cellulosic ethanol from sugarcane has the potential to substantially decrease the cost of biofuel production and significantly reduce greenhouse gas emissions. The research proposed here will advance our ability to improve sugarcane through biotechnology.Read moreRead less
Understanding the control of male germ-line development by the germline-restrictive silencing factor in plants. The world population is currently increasing at an unprecedented rate, with a concomitant requirement to double the food production from the same amount of arable land. To ensure global political and social stability, equitably increasing sustainable food production without compromising environmental integrity remains a major challenge. This proposal investigates the molecular mechanis ....Understanding the control of male germ-line development by the germline-restrictive silencing factor in plants. The world population is currently increasing at an unprecedented rate, with a concomitant requirement to double the food production from the same amount of arable land. To ensure global political and social stability, equitably increasing sustainable food production without compromising environmental integrity remains a major challenge. This proposal investigates the molecular mechanisms underlying male germ line initiation and development in plants. Switching off male gamete development in some crop plants will create male sterile lines, which, when crossed with genetically distinct lines, will have the potential to produce hybrids that yield 20-30 percent more crop without additional inputs. Read moreRead less