Nuclear RNA surveillance and its connection to splicing quality control. Due to the error-prone nature of RNA splicing, elaborate quality control processes ensure that only correctly spliced transcripts can leave the nucleus. It has long been known that incorrectly spliced mRNA transcripts are degraded by the nuclear RNA surveillance machinery, but how the RNA quality control machinery is connected to nuclear RNA surveillance is not known. This proposal aims to uncover the connection between the ....Nuclear RNA surveillance and its connection to splicing quality control. Due to the error-prone nature of RNA splicing, elaborate quality control processes ensure that only correctly spliced transcripts can leave the nucleus. It has long been known that incorrectly spliced mRNA transcripts are degraded by the nuclear RNA surveillance machinery, but how the RNA quality control machinery is connected to nuclear RNA surveillance is not known. This proposal aims to uncover the connection between these two important processes and will fill a significant gap in our understanding of how splicing quality control and nuclear RNA surveillance work. The project will also identify sequence features that trigger abortive splicing reactions and will thus help to improve the design of synthetic mRNAs.Read moreRead less
Role of R-loops and double R-loops in genome organisation and transcription. The majority of our genome is converted to an extensive network of non-protein-coding RNA molecules (ncRNAs), but the function of these ncRNAs is unknown. This project aims to identify and determine the mechanism of action of nuclear ncRNA networks with a particular focus on nuclear ncRNAs that form RNA-DNA hybrids with the genomic DNA. These studies have the potential to lead to ground-breaking discoveries in our under ....Role of R-loops and double R-loops in genome organisation and transcription. The majority of our genome is converted to an extensive network of non-protein-coding RNA molecules (ncRNAs), but the function of these ncRNAs is unknown. This project aims to identify and determine the mechanism of action of nuclear ncRNA networks with a particular focus on nuclear ncRNAs that form RNA-DNA hybrids with the genomic DNA. These studies have the potential to lead to ground-breaking discoveries in our understanding of genome organisation and the mechanism of transcription control, and might provide an entirely new tool-box to manipulate genome function. This should provide significant benefits to efforts to develop innovative biotechnology and genome editing technologies in plants and animals.Read moreRead less
RNA surveillance and the initial steps of RNA biogenesis. This project aims to understand the initial steps of RNA biogenesis and how this process is linked to the chromatin environment. Although less than five per cent of our genome encodes proteins, almost the entire genome is transcribed to RNA. A large portion of these transcripts are degraded during the early steps of RNA biogenesis by the RNA surveillance machinery, but the mechanism for the recognition and degradation of these transcripts ....RNA surveillance and the initial steps of RNA biogenesis. This project aims to understand the initial steps of RNA biogenesis and how this process is linked to the chromatin environment. Although less than five per cent of our genome encodes proteins, almost the entire genome is transcribed to RNA. A large portion of these transcripts are degraded during the early steps of RNA biogenesis by the RNA surveillance machinery, but the mechanism for the recognition and degradation of these transcripts is not understood. New evidence suggests that the chromatin environment of the transcribed locus plays an important role in this process. This project will lead to significant benefits in the implementation of emerging RNA-based technologies and in understanding how genome stability is maintained.Read moreRead less
How and why cells decorate their genetic messages. This project aims to investigate a new layer of genomic control mediated not by DNA but instead by chemical modifications found on the cell's working copies of genetic information called messenger RNA. The investigations will use cutting-edge RNA sequencing technology and the fruit fly model organism to uncover the scope and mechanisms by which such modifications enact their roles at the molecular level and within the body plan of an animal. Exp ....How and why cells decorate their genetic messages. This project aims to investigate a new layer of genomic control mediated not by DNA but instead by chemical modifications found on the cell's working copies of genetic information called messenger RNA. The investigations will use cutting-edge RNA sequencing technology and the fruit fly model organism to uncover the scope and mechanisms by which such modifications enact their roles at the molecular level and within the body plan of an animal. Expected outcomes include novel molecular tools and models that will assist in understanding and manipulating the function of genomes. Such knowledge should provide benefits in developing innovative biotechnology applications of use in human health, agriculture and managing the environment.
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Beyond Scenarios: Testable Models of the Evolution of Norms. The aim of this project is to investigate the evolution of social norms, and their causal role in social life and its breakdown. It expects to generate new knowledge in this area through the application of new formal techniques to existing hypotheses; especially causal analysis, evolutionary game theory, and phylogenetic cross-cultural testing for empirically plausibility. Expected outcomes include theory development, improved research ....Beyond Scenarios: Testable Models of the Evolution of Norms. The aim of this project is to investigate the evolution of social norms, and their causal role in social life and its breakdown. It expects to generate new knowledge in this area through the application of new formal techniques to existing hypotheses; especially causal analysis, evolutionary game theory, and phylogenetic cross-cultural testing for empirically plausibility. Expected outcomes include theory development, improved research infrastructure and training in collaboration with international partners, and theoretical recommendations for policy intervention. This should allow greater insight and control over the levers of peaceful social life, both in traditional societies, and in large, open, multi-cultural nations like Australia. Read moreRead less
How novel ribosomal RNA gene repeat variants drive cellular function. The hundreds of ribosomal RNA gene repeat copies are a remarkable part of our genomes, as they encode the machinery responsible for all cellular protein synthesis and shape the structure of the nucleus. However, due to their high degree of sequence similarity, they still have not been assembled into the human genome reference. This project will resolve this impasse and furthermore uncover the functional impacts of a newly iden ....How novel ribosomal RNA gene repeat variants drive cellular function. The hundreds of ribosomal RNA gene repeat copies are a remarkable part of our genomes, as they encode the machinery responsible for all cellular protein synthesis and shape the structure of the nucleus. However, due to their high degree of sequence similarity, they still have not been assembled into the human genome reference. This project will resolve this impasse and furthermore uncover the functional impacts of a newly identified molecular diversity in the ribosomal RNA gene repeats. Outcomes include new paradigms for how the ribosomal RNA gene repeats drive protein synthesis and genome structure, and a blueprint to develop novel genomics applications for human health, biotechnology, and agriculture.Read moreRead less
The roles and regulators of new plant cells linked to root transport. Plant genomics has moved to the single cell resolution, allowing precise investigations of previously hidden cell types and cell states that respond to environmental stress and that vary among differentially adapted plant populations. Here, we will extend our pioneering efforts that have mapped and discovered novel root cell types, to determine their salt and nutrient stress responses, and to elegantly dissect the underling ca ....The roles and regulators of new plant cells linked to root transport. Plant genomics has moved to the single cell resolution, allowing precise investigations of previously hidden cell types and cell states that respond to environmental stress and that vary among differentially adapted plant populations. Here, we will extend our pioneering efforts that have mapped and discovered novel root cell types, to determine their salt and nutrient stress responses, and to elegantly dissect the underling causal genetic variation. The unique cell markers and regulatory networks will be validated with tissue specific and transgenic tools that can work across a host of plant species to reveal adaptive cellular responses to harsh environmental conditions.Read moreRead less
Tibet's rivers in the Anthropocene: history and present trajectories. This project aims to produce a multifaceted history of the eastern Tibetan Plateau's rivers, focusing on the increasing human impacts during the Anthropocene. It will combine data from archival, cultural and oral sources in multiple languages with the results of scientific studies of river flow, water quality, and sediment, ice, and tree-rings analysis. The project will produce both historical narratives and graphic representa ....Tibet's rivers in the Anthropocene: history and present trajectories. This project aims to produce a multifaceted history of the eastern Tibetan Plateau's rivers, focusing on the increasing human impacts during the Anthropocene. It will combine data from archival, cultural and oral sources in multiple languages with the results of scientific studies of river flow, water quality, and sediment, ice, and tree-rings analysis. The project will produce both historical narratives and graphic representations that model past land and water usage. The results of the project will underpin environmental policy for this hydrologically and ecologically crucial region, including the development of a paradigm of care based on the region's indigenous cultural resources.Read moreRead less
Living on the edge: how do Australian plants cope with extreme temperature? Of all the climatic factors determining species distributions, temperature is arguably the most important. It is extremes – rather than averages – that drive species evolution. So it is concerning that although extreme temperature events are increasing in frequency and intensity little is known about the breadth of thermal tolerance of plants from extreme environments. This information is crucial to understand species di ....Living on the edge: how do Australian plants cope with extreme temperature? Of all the climatic factors determining species distributions, temperature is arguably the most important. It is extremes – rather than averages – that drive species evolution. So it is concerning that although extreme temperature events are increasing in frequency and intensity little is known about the breadth of thermal tolerance of plants from extreme environments. This information is crucial to understand species distribution and survival under future climate regimes. This project will ascertain the thermal breadth of Australian species growing in situ and under controlled environments. The project will contribute to development of effective conservation, restoration and rehabilitation plans for Australian native plant communities. Read moreRead less