Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed prod ....Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed production is not currently available. In wheat alone, apomixis presents for Australia an economic value of more than Aus$ ½ billion per annum. Furthermore, controlled apomixis will accelerate breeding programs that will bring drought resistance and minimal fertiliser requiring varieties to the farmer.Read moreRead less
Accelerating the genetic improvement of grain legumes for Australia by developing doubled haploid technology for field pea and chickpea. Doubled haploid technology is used in many broad acre crop species to accelerate cultivar development and create homozygous populations for genetic mapping. Field pea and chickpea have been unresponsive to this technique but a recent breakthrough by UWA researchers has resulted in haploid pro-embryos from in vitro cultured immature pollen. A barrier to further ....Accelerating the genetic improvement of grain legumes for Australia by developing doubled haploid technology for field pea and chickpea. Doubled haploid technology is used in many broad acre crop species to accelerate cultivar development and create homozygous populations for genetic mapping. Field pea and chickpea have been unresponsive to this technique but a recent breakthrough by UWA researchers has resulted in haploid pro-embryos from in vitro cultured immature pollen. A barrier to further embryo maturation has been identified, which we propose to overcome using powerful microscopy tools to elucidate haploid embryology processes. This information will be applied to develop world-first in vitro doubled haploid protocols for these species, which will facilitate the development and accelerated delivery to industry of better adapted, high yielding cultivars.Read moreRead less
Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a nov ....Role of alanine aminotransferase in improved nitrogen use efficiency (NUE) in cereals. The use of nitrogen-based fertilisers by crop plants is poor where efficiencies (nitrogen taken up to that applied) is often less than 40%. Nitrogen not used is often lost to the environment through leaching and or volatilisation. Improving nitrogen use efficiency (NUE) in agriculture will decrease overall nitrogen fertiliser use and minimise its environmental footprint. This project will characterise a novel NUE technology that when transferred to plants significantly improves NUE. We will define the phenotype at the molecular, biochemical and physiological levels to maximise its adoption to other agricultural crops such as wheat, barley and maize.Read moreRead less
Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-valu ....Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-value alternative products. In addition, cellulosic ethanol from sugarcane has the potential to substantially decrease the cost of biofuel production and significantly reduce greenhouse gas emissions. The research proposed here will advance our ability to improve sugarcane through biotechnology.Read moreRead less
Improved Indian Mustard for sustainable biodiesel production. There is an urgent need to reduce Australia's dependency on fossil fuels and to improve the sustainability of the Australian farming sector. The on-farm production of biodiesel will reduce farm input costs, reduce farmer dependency on fluctuations in fossil fuel costs and reduce greenhouse gas emissions. Indian mustard is drought tolerant and produces inedible oil suitable for biodiesel production. The genetic improvement of Indian mu ....Improved Indian Mustard for sustainable biodiesel production. There is an urgent need to reduce Australia's dependency on fossil fuels and to improve the sustainability of the Australian farming sector. The on-farm production of biodiesel will reduce farm input costs, reduce farmer dependency on fluctuations in fossil fuel costs and reduce greenhouse gas emissions. Indian mustard is drought tolerant and produces inedible oil suitable for biodiesel production. The genetic improvement of Indian mustard will provide farmers with a viable crop for biodiesel production and its bio-fumigation properties make it an effective addition to the crop rotation. Useful by-products include a natural insecticide and, once the glucosinolates are removed, meal suitable for animal feed. Read moreRead less
Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through ....Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through helping ensure the profitability of vital Australian export agricultural industries and the sustainability of the soil/land resource. This proposal will ensure that Australia leads international herbicide resistance research and will enable the capture of intellectual property and commercial opportunities. Read moreRead less
Targeted approaches to improve nitrogen use efficiency in maize. Nitrogen is an essential input required for growing high yielding quality cereal crops such as maize and wheat. Unfortunately, excessive use of nitrogen fertilizers can lead to serious environmental costs including nitrogen pollution through leaching and the significant cost in non-renewable fossil fuels used in their production. Improving nitrogen use efficiency in crops such as maize will reduce fertilizer use while ensuring lo ....Targeted approaches to improve nitrogen use efficiency in maize. Nitrogen is an essential input required for growing high yielding quality cereal crops such as maize and wheat. Unfortunately, excessive use of nitrogen fertilizers can lead to serious environmental costs including nitrogen pollution through leaching and the significant cost in non-renewable fossil fuels used in their production. Improving nitrogen use efficiency in crops such as maize will reduce fertilizer use while ensuring long-term sustainable production and harvestable yields. This collaboration with DuPont-Pioneer will focus on identifying nitrogen-linked traits in Maize that will be incorporated into new lines targeted at reducing grower dependence on nitrogen fertilizers.Read moreRead less
Using defined biotic and abiotic stimuli to dissect patterns of gene expression and protein accumulation that specify root architecture. Root morphogenesis is fundamental to agriculture and valuable for investigating the informational networks of genes, proteins and metabolites that control root growth and plant development. Root systems vary widely both within and between species. Root morphology is directed by a basic genetic program that is influenced by environmental factors to provide an e ....Using defined biotic and abiotic stimuli to dissect patterns of gene expression and protein accumulation that specify root architecture. Root morphogenesis is fundamental to agriculture and valuable for investigating the informational networks of genes, proteins and metabolites that control root growth and plant development. Root systems vary widely both within and between species. Root morphology is directed by a basic genetic program that is influenced by environmental factors to provide an enormous "phenotypic plasticity". This project will use two model plant systems to investigate how different external signals are "translated" by the plant into different developmental regimes. This knowledge is crucial to understanding how the plasticity of root development is modulated in response to changing environmental factors.Read moreRead less
Australian native plant species as models for understanding the regulation and roles of betalain pigment synthesis. This project will have triple bottom line benefits. Economic: new cultivars developed from understanding betalains will sell worldwide, generating returns, incomes and employment in urban, peri-urban and rural Australia. Social: Novel Ptilotus and Calandrinia cultivars will adorn parks and gardens around Australia, thereby enhancing quality of life. Environmental: Ptilotus and Cala ....Australian native plant species as models for understanding the regulation and roles of betalain pigment synthesis. This project will have triple bottom line benefits. Economic: new cultivars developed from understanding betalains will sell worldwide, generating returns, incomes and employment in urban, peri-urban and rural Australia. Social: Novel Ptilotus and Calandrinia cultivars will adorn parks and gardens around Australia, thereby enhancing quality of life. Environmental: Ptilotus and Calandrinia are inherently drought resistant. Novel ornamental cultivars will have high water use efficiency and contribute to water conservation in urban Australia in the face of drought and global warming. Moreover, understanding their synthesis and roles could yield new strategies for enhancing stress tolerance in other species, including crop plants.Read moreRead less
A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants w ....A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants with nematodes. It is based on an Australian patent which has wide applicability in plant biotechnology. Nematode control will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less