Metabolic engineering of sugarcane: production of a biodegradable bioplastic as a test-case. The aim of this project is to produce a commercially valuable bioplastic called PHB in transgenic sugarcane. This bioplastic has similar properties to petrochemically produced polypropylene, but is fully biodegradable. PHB has been produced in the model plant Arabidopsis thaliana at commercially viable levels. The expected outcome of high-level production of PHB in sugarcane would have significant ben ....Metabolic engineering of sugarcane: production of a biodegradable bioplastic as a test-case. The aim of this project is to produce a commercially valuable bioplastic called PHB in transgenic sugarcane. This bioplastic has similar properties to petrochemically produced polypropylene, but is fully biodegradable. PHB has been produced in the model plant Arabidopsis thaliana at commercially viable levels. The expected outcome of high-level production of PHB in sugarcane would have significant benefits to the Australian sugar industry, the rural economy, and the Australian environment.Read moreRead less
Tailored Biodegradable Polymers for Injection Moulding Applications. This project aims to engineer and scale up production of biodegradable injection molded products with tailored properties, processing and biodegradation. Specifically we will focus on novel materials and processing technologies in tandem with biodegradation understandings to expand the utilization of starch-based polymers. We will then combine fundamental rheological and polymer processing skills with and product development ex ....Tailored Biodegradable Polymers for Injection Moulding Applications. This project aims to engineer and scale up production of biodegradable injection molded products with tailored properties, processing and biodegradation. Specifically we will focus on novel materials and processing technologies in tandem with biodegradation understandings to expand the utilization of starch-based polymers. We will then combine fundamental rheological and polymer processing skills with and product development experience from both universities and the industrial partner (Plantic Technologies) to scale up processing and develop successful biodegradable products. This project will also enable an Australian owned start-up company with a broad shareholder base to generate significant export income through key commercialization.Read moreRead less
Understanding and avoiding transgene silencing in sugarcane. Sugarcane is one of the world's major crops for food (sugar) and fuel (ethanol, electricity co-generation). It is one of the most appealing target crops for metabolic engineering aimed at renewable biomaterials and biofuels. Australia has invested strongly to achieve scientific leadership in gene technologies in our major export crops including sugarcane. Field tests show that development of methods to avoid unstable expression or 'sil ....Understanding and avoiding transgene silencing in sugarcane. Sugarcane is one of the world's major crops for food (sugar) and fuel (ethanol, electricity co-generation). It is one of the most appealing target crops for metabolic engineering aimed at renewable biomaterials and biofuels. Australia has invested strongly to achieve scientific leadership in gene technologies in our major export crops including sugarcane. Field tests show that development of methods to avoid unstable expression or 'silencing' of introduced genes is now a critical requirement for practical application. The current project emerges from industry recognition of the need to understand and avoid transgene silencing. The methods developed using sugarcane are expected to have rapid applicability for wider benefits in agriculture.Read moreRead less
Optimising transgene expression and stability for enhanced sugar yield and high-value sugar production in sugarcane. 'SugarBooster' technology has the potential to underpin a value-added sugarcane industry. Higher sucrose yield is a key to sustainable export profitability, and it makes the development of renewable biofuels from sugarcane more feasible. Isomaltulose has established health benefits for consumers and it is also attractive as a renewable starting material for industry. But it must c ....Optimising transgene expression and stability for enhanced sugar yield and high-value sugar production in sugarcane. 'SugarBooster' technology has the potential to underpin a value-added sugarcane industry. Higher sucrose yield is a key to sustainable export profitability, and it makes the development of renewable biofuels from sugarcane more feasible. Isomaltulose has established health benefits for consumers and it is also attractive as a renewable starting material for industry. But it must currently be produced by expensive fermentation. Efficient production in plants will open an increasing world market. This collaborative project is vital to bring these breakthrough technologies to reliable commercial implementation, in time to capture the economic benefits of the protected IP for Australia.Read moreRead less
Artificial Intelligence Based Deterioration Model for Development of Bridge Network Maintenance Strategy. The proposed AI-based methodology in conjunction with a Bridge Management System can tailor-make bridge deterioration models for a given bridge authority. The models so produced will enable effective BMS implementation which generates missing inspection records of past years, establishes optimal MR&R strategies and then reliably forecasts future bridge condition ratings. The methodology will ....Artificial Intelligence Based Deterioration Model for Development of Bridge Network Maintenance Strategy. The proposed AI-based methodology in conjunction with a Bridge Management System can tailor-make bridge deterioration models for a given bridge authority. The models so produced will enable effective BMS implementation which generates missing inspection records of past years, establishes optimal MR&R strategies and then reliably forecasts future bridge condition ratings. The methodology will be verified using available bridge datasets of QDMR and GCCC. The methodology is applicable to other bridge authorities throughout Australia and internationally to maintain ageing bridge stock. Read moreRead less
Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-valu ....Optimization of Transgene Expression in Sugarcane. Sugarcane is one of Australia's most important crops. However, worldwide competition and declining sugar prices threaten the long term economic sustainability of this industry unless alternative markets for sugarcane are created. Biotechnology holds the greatest promise for the development of an economically sustainable sugarcane industry through the production of varieties that can be used for cellulosic ethanol or as biofactories for high-value alternative products. In addition, cellulosic ethanol from sugarcane has the potential to substantially decrease the cost of biofuel production and significantly reduce greenhouse gas emissions. The research proposed here will advance our ability to improve sugarcane through biotechnology.Read moreRead less
Combining molecular plant physiology and breeding to improve canola (Brassica napus) performance in dry environments. Canola is Australia's most important oilseed crop with 1,400,000 hectares sown annually worth $560m. The major abiotic factor limiting canola production in Australia is water availability. Transpiration efficiency (TE) is a trait with potential to contribute to improved drought tolerance of grain crops. This project seeks to select canola germplasm with improved TE by indirect s ....Combining molecular plant physiology and breeding to improve canola (Brassica napus) performance in dry environments. Canola is Australia's most important oilseed crop with 1,400,000 hectares sown annually worth $560m. The major abiotic factor limiting canola production in Australia is water availability. Transpiration efficiency (TE) is a trait with potential to contribute to improved drought tolerance of grain crops. This project seeks to select canola germplasm with improved TE by indirect selection for carbon-isotope-discrimination. In addition we will use the extensive Brassica-Arabidopsis genome synteny to locate and alter the expression of genes involved in TE using Arabidopsis as a model. The longterm aim is to improve the reliability and overall grain production of canola in Australia. Read moreRead less
Genetic transformation of the biodiesel producing tree legume Pongamia pinnata. In response to global climate change and the threat of declining reserves of fossil fuels, liquid fuels of the future are to be manufactured in greater proportion from sustainable biological resources. Pongamia, a native legume tree, has the potential to make a significantly positive impact on the emerging biofuels industry. As a legume Pongamia requires no nitrogen fertiliser, is not a food crop, and can grow well o ....Genetic transformation of the biodiesel producing tree legume Pongamia pinnata. In response to global climate change and the threat of declining reserves of fossil fuels, liquid fuels of the future are to be manufactured in greater proportion from sustainable biological resources. Pongamia, a native legume tree, has the potential to make a significantly positive impact on the emerging biofuels industry. As a legume Pongamia requires no nitrogen fertiliser, is not a food crop, and can grow well on marginal lands unlikely to be used for food crops. Improvement of Pongamia through the genetic tools developed in this project will provide an environmentally sustainable source of biodiesel long into the future.Read moreRead less
BIOCATALYSTS MINED FROM CYTOCHROME P450 LIBRARIES: AN INNOVATIVE TOOL FOR ACCELERATING DRUG DEVELOPMENT. The cytochrome P450s (P450s) are a family of enzymes that are perhaps the most versatile biological catalysts known. DNA shuffling is an emerging technique that takes the genes encoding families of enzymes and creates libraries of catalysts with both improved and novel properties. We will obtain proof of concept that shuffled P450 libraries can be screened and optimized for use as biocatalys ....BIOCATALYSTS MINED FROM CYTOCHROME P450 LIBRARIES: AN INNOVATIVE TOOL FOR ACCELERATING DRUG DEVELOPMENT. The cytochrome P450s (P450s) are a family of enzymes that are perhaps the most versatile biological catalysts known. DNA shuffling is an emerging technique that takes the genes encoding families of enzymes and creates libraries of catalysts with both improved and novel properties. We will obtain proof of concept that shuffled P450 libraries can be screened and optimized for use as biocatalysts in drug development. The methodologies developed here will overcome two critical bottlenecks in current drug development: the optimisation and metabolic profiling of new drug candidates. This will yield important benefits in accelerating the optimisation and safety testing of drugs under development.Read moreRead less
Accelerated breeding for a changing environment: genomic and physiological profiling of newly generated polyploid trees. Global climate change threatens the health and productivity of forests and plantations. Because tree breeding is slow, elite trees cannot be adapted rapidly to new environments. A new procedure for accelerated tree breeding has been developed by the industry partner. The procedure, termed polyploidisation, increases DNA content and produces novel traits that can improve plant ....Accelerated breeding for a changing environment: genomic and physiological profiling of newly generated polyploid trees. Global climate change threatens the health and productivity of forests and plantations. Because tree breeding is slow, elite trees cannot be adapted rapidly to new environments. A new procedure for accelerated tree breeding has been developed by the industry partner. The procedure, termed polyploidisation, increases DNA content and produces novel traits that can improve plant growth and resilience. Polyploidisation is a natural force in plant evolution and its routine application for tree breeding has much potential. Using diverse approaches, we will investigate how newly synthesised polyploid tree species perform under heat and drought stress.Read moreRead less