The ecological dynamics of secreted bacteriocins and the evolution of multiple bacteriocin production in Escherichia coli. Bacteria produce compounds known as bacteriocins that are toxic to other microorganisms. The success of bacteria as bio-control agents and in probiotic formulations for the control microbial pathogens is, in part, due to bacteriocins. Bacteriocins may also have a role as replacements for traditional antibiotics and as natural food preservatives. The potential usefulness of b ....The ecological dynamics of secreted bacteriocins and the evolution of multiple bacteriocin production in Escherichia coli. Bacteria produce compounds known as bacteriocins that are toxic to other microorganisms. The success of bacteria as bio-control agents and in probiotic formulations for the control microbial pathogens is, in part, due to bacteriocins. Bacteriocins may also have a role as replacements for traditional antibiotics and as natural food preservatives. The potential usefulness of bacteriocins as the active agent in bio-control agents, as antibiotic replacements, as food preservatives, and as part of the repertoire of traits in probiotic formulations requires a sound understanding of the eco-evolutionary dynamics of bacteriocins. Understanding the ecology and evolution of bacteriocins is the goal of the proposed research.Read moreRead less
Microbial junk food: developing synthetic platforms for plastic degradation. This project aims to establish the genetic basis of polyethelene biodegradation (PED) by microbes from the gut microbiome of plastic-eating caterpillars. It will transform the active microbial PED genes into carefully designed synthetic microbes for efficient, safe and large-scale PED. The project will combine innovative functional microbial genetic tools and synthetic biology techniques with solid biochemistry and bioi ....Microbial junk food: developing synthetic platforms for plastic degradation. This project aims to establish the genetic basis of polyethelene biodegradation (PED) by microbes from the gut microbiome of plastic-eating caterpillars. It will transform the active microbial PED genes into carefully designed synthetic microbes for efficient, safe and large-scale PED. The project will combine innovative functional microbial genetic tools and synthetic biology techniques with solid biochemistry and bioinformatics to produce translatable synthetic platforms containing key genes optimised for efficient PE waste removal. The outcomes will have the potential to transform the relative ineffective and expensive current methods for PE disposal into flexible, cost-effective and sustainable solutions applicable to multiple sectors.Read moreRead less
Revealing the evolutionary and ecological dynamics of avian influenza virus. This project aims to understand how avian influenza virus (AIV) emerges, evolves and spreads in wild birds. AIV has the potential to devastate the poultry industry and cause human pandemics, but the factors that shape the genetic diversity of AIV in its wild bird reservoir are poorly understood. The project plans to combine genomic, ecological and phylogenetic approaches to reveal key aspects of AIV evolution, as well a ....Revealing the evolutionary and ecological dynamics of avian influenza virus. This project aims to understand how avian influenza virus (AIV) emerges, evolves and spreads in wild birds. AIV has the potential to devastate the poultry industry and cause human pandemics, but the factors that shape the genetic diversity of AIV in its wild bird reservoir are poorly understood. The project plans to combine genomic, ecological and phylogenetic approaches to reveal key aspects of AIV evolution, as well as the risk for future viral emergence. Using sampling sites in Australia and Antarctica, it plans to investigate AIV diversity, the evolutionary dynamics of AIV in wild birds and poultry, and the role played by environmental transmission in AIV ecology.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE240100388
Funder
Australian Research Council
Funding Amount
$437,977.00
Summary
Ecological and phylogenomic insights into infectious diseases in animals. This project aims to address major knowledge gaps in our understanding of Clostridium difficile, a leading cause of severe gastrointestinal disease in animals. The project is expected to define the epidemiology of C. difficile infection in Australian horses, characterise the genetic and phenotypic traits of C. difficile strains causing equine disease and develop a new tool for enhanced genomic tracking of C. difficile in a ....Ecological and phylogenomic insights into infectious diseases in animals. This project aims to address major knowledge gaps in our understanding of Clostridium difficile, a leading cause of severe gastrointestinal disease in animals. The project is expected to define the epidemiology of C. difficile infection in Australian horses, characterise the genetic and phenotypic traits of C. difficile strains causing equine disease and develop a new tool for enhanced genomic tracking of C. difficile in animals. These outcomes will support strategies by the veterinary sector to improve the detection, prevention and control of C. difficile infections in animals, providing long-term socio-economic benefits arising from reduced incidence and mortality associated with C. difficile infections in Australian horses and livestock.Read moreRead less
Bacterial polycyclic aromatic hydrocarbon transport and degradation. This project aims to investigate the molecular processes underpinning the degradation of polycyclic aromatic hydrocarbons (PAHs) by bacteria. PAHs are persistent environmental contaminants linked to several human diseases, including cancer. Bacteria capable of degrading PAHs could be used to naturally and effectively reduce environmental PAH loads to below safe levels. The project will apply techniques in functional genomics an ....Bacterial polycyclic aromatic hydrocarbon transport and degradation. This project aims to investigate the molecular processes underpinning the degradation of polycyclic aromatic hydrocarbons (PAHs) by bacteria. PAHs are persistent environmental contaminants linked to several human diseases, including cancer. Bacteria capable of degrading PAHs could be used to naturally and effectively reduce environmental PAH loads to below safe levels. The project will apply techniques in functional genomics and biochemistry to help define the ways that PAHs are taken up from the environment by bacteria, their fate within bacterial cells, and the ways that bacteria overcome the inherent toxicity of PAHs. The knowledge generated is expected to enhance our capacity to rationally deploy bacteria for PAH degradation.Read moreRead less
Regulation of saxitoxin production in bacteria and algae. In Australia, toxic algal blooms have had a devastating impact on marine and freshwater resources. In collaboration with a biotechnology company, this project will develop exciting new methods based on information regarding the genetics of the toxin, to monitor and potentially mitigate the effects of algal blooms on water supplies and aquaculture industries. We will use this method to determine the impact of light and salinity in regulati ....Regulation of saxitoxin production in bacteria and algae. In Australia, toxic algal blooms have had a devastating impact on marine and freshwater resources. In collaboration with a biotechnology company, this project will develop exciting new methods based on information regarding the genetics of the toxin, to monitor and potentially mitigate the effects of algal blooms on water supplies and aquaculture industries. We will use this method to determine the impact of light and salinity in regulating toxin production in cyanobacteria and algae.Read moreRead less
Managing acid mine drainage in northern Australia using microbial mats. One of the most difficult environmental issues for the mining industry is acid mine drainage (AMD) that can lead to significant environmental damage. This project aims to identify microbes and characterise their roles in AMD formation in north Australia. We will use our new knowledge to design and trial microbial mats for the treatment of AMD. A successful AMD microbial treatment technology will minimise the risk of acid run ....Managing acid mine drainage in northern Australia using microbial mats. One of the most difficult environmental issues for the mining industry is acid mine drainage (AMD) that can lead to significant environmental damage. This project aims to identify microbes and characterise their roles in AMD formation in north Australia. We will use our new knowledge to design and trial microbial mats for the treatment of AMD. A successful AMD microbial treatment technology will minimise the risk of acid runoff and metal seepage into rivers and through groundwater. AMD treatment technology we develop in the tropics where we experience the extremes of dry and wet seasons will require only minor modification to operate in temperate climates however the reverse is not true. Read moreRead less
Can cyanobacteria use organic nutrients to thrive in future oceans? Marine cyanobacteria are central to regulating the global climate and underpin entire marine food webs. Though they possess genes necessary to uptake diverse organic nutrients, we know very little about whether and how organic nutrients shape the physiology and ecology of cyanobacteria. Using our innovative high-throughput approach, this project aims to systematically characterise organic nutrient uptake in picocyanobacteria. O ....Can cyanobacteria use organic nutrients to thrive in future oceans? Marine cyanobacteria are central to regulating the global climate and underpin entire marine food webs. Though they possess genes necessary to uptake diverse organic nutrients, we know very little about whether and how organic nutrients shape the physiology and ecology of cyanobacteria. Using our innovative high-throughput approach, this project aims to systematically characterise organic nutrient uptake in picocyanobacteria. Our molecules-to-ecosystems approach expects to transform our understanding of alternate nutrient acquisition in cyanobacteria and how it may shape populations of these important photosynthetic organisms in a rapidly-changing ocean landscape. Read moreRead less
Bacterial communities in metropolitan, rural and indigenous Australians. This project aims to apply recently developed culturing methods to archive and phenotype bacterial species found in the human gut in a representative sample from metropolitan, rural and indigenous Australians. This project expects to isolate, genome sequence, classify, characterize and permanently archive 1500 bacterial species. Expected outcomes of this project include detailed knowledge of previously undiscovered bacteria ....Bacterial communities in metropolitan, rural and indigenous Australians. This project aims to apply recently developed culturing methods to archive and phenotype bacterial species found in the human gut in a representative sample from metropolitan, rural and indigenous Australians. This project expects to isolate, genome sequence, classify, characterize and permanently archive 1500 bacterial species. Expected outcomes of this project include detailed knowledge of previously undiscovered bacterial species, improved methods to measure the bacterial species that inhabit the human gut and a detailed understanding of the gut microbiota of Australians. This project should significantly enhance our knowledge of bacterial diversity and evolution and provide detailed insights into bacterial transmission.Read moreRead less
The role of dysregulated signalling by TORC1 in mitochondrial disease. The mitochondria are tiny subcellular compartments responsible for producing over 90 per cent of the cell's energy. Mitochondrial defects feature both in genetic diseases that directly affect the mitochondria and in most neurodegenerative diseases. These incurable diseases are expected to eclipse cancer as the second major cause of death worldwide by 2040. Using a simple model organism, Dictyostelium, previous research showed ....The role of dysregulated signalling by TORC1 in mitochondrial disease. The mitochondria are tiny subcellular compartments responsible for producing over 90 per cent of the cell's energy. Mitochondrial defects feature both in genetic diseases that directly affect the mitochondria and in most neurodegenerative diseases. These incurable diseases are expected to eclipse cancer as the second major cause of death worldwide by 2040. Using a simple model organism, Dictyostelium, previous research showed that dysregulated intracellular signalling by a cellular energy-sensing alarm protein is responsible for diverse cellular pathologies in mitochondrially diseased cells. This project will determine the role in these pathways of a second cellular stress-sensing protein complex, TORC1. New treatment possibilities may emerge.Read moreRead less