Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this ....Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this study will be a catalogue of functionally active circRNAs. Over the past decades, the wealth of knowledge on the function of linear mRNAs has had a significant impact on medicine and agriculture. Similarly understanding how circRNAs regulate cellular activities may have an analogous impact on humans.Read moreRead less
Defining how molecular switches program cell identity during development. Aims: This project aims to investigate how molecular switches known as transcription factors, work together to turn genes on or off to program cell identity during development.
Significance: This project expects to generate new knowledge in the area of genetics and developmental biology using collaborative, cutting edge technologies.
Outcomes: Expected outcomes of this project include the identification of new genes impor ....Defining how molecular switches program cell identity during development. Aims: This project aims to investigate how molecular switches known as transcription factors, work together to turn genes on or off to program cell identity during development.
Significance: This project expects to generate new knowledge in the area of genetics and developmental biology using collaborative, cutting edge technologies.
Outcomes: Expected outcomes of this project include the identification of new genes important for programming the identity of cells that comprise our blood vessels, lymphatic vessels and circulating blood cells.
Benefits: Data generated will underpin the development of approaches to program/reprogram stem cells to produce mature cells for transplantation or tissue engineering purposes ex vivo.Read moreRead less
Unique parental epitranscriptome states regulate seed development. This project aims to investigate how developing central cell epitranscriptomes are linked to seed growth, how the cell regulates the unique epigenetic states, and the role of the system in driving phenotypic diversity. Maternal and paternal effects determine growth and development of multicellular angiosperm plants. Previous work has discovered unique ribonucleic acid (RNA) epitranscriptome states dependent on the parent-of-origi ....Unique parental epitranscriptome states regulate seed development. This project aims to investigate how developing central cell epitranscriptomes are linked to seed growth, how the cell regulates the unique epigenetic states, and the role of the system in driving phenotypic diversity. Maternal and paternal effects determine growth and development of multicellular angiosperm plants. Previous work has discovered unique ribonucleic acid (RNA) epitranscriptome states dependent on the parent-of-origin in developing central cell that gives rise to the endosperm tissue of the seed that impacts on growth of the seed. This project expects to provide economic benefits by increasing yield of agricultural crops during increasingly challenging conditions.Read moreRead less
Whole-genome multivariate reaction norm model for complex traits. This project aims to develop a multivariate whole-genome genotype-covariate correlation and interaction model that can be applied to a wide range of existing genome-wide association study (GWAS) datasets. Genotype-covariate correlation and interaction (GCCI) are fundamental in biology but there is no standard approach to disentangle interaction from correlation in the whole-genome analyses. This project will address the key featur ....Whole-genome multivariate reaction norm model for complex traits. This project aims to develop a multivariate whole-genome genotype-covariate correlation and interaction model that can be applied to a wide range of existing genome-wide association study (GWAS) datasets. Genotype-covariate correlation and interaction (GCCI) are fundamental in biology but there is no standard approach to disentangle interaction from correlation in the whole-genome analyses. This project will address the key feature in biology, which relates to dissecting the complex mechanism of association and interaction. The proposed statistical model implemented in a context of a novel design based on multiple GWAS data sets is a paradigm shifting-tool with applications to multiple industries.Read moreRead less
Revealing molecular detail of DNA triplexes to underpin antigene technology. Variations from the classic DNA double helix structure are proposed to play key roles in a range of cellular processes, particularly gene regulation. However, the biological function and therapeutic potential of these unusual DNA structures are poorly explored, since the fundamental molecular details which govern their formation and interactions with cellular machinery are not well described. This project aims to develo ....Revealing molecular detail of DNA triplexes to underpin antigene technology. Variations from the classic DNA double helix structure are proposed to play key roles in a range of cellular processes, particularly gene regulation. However, the biological function and therapeutic potential of these unusual DNA structures are poorly explored, since the fundamental molecular details which govern their formation and interactions with cellular machinery are not well described. This project aims to develop innovative methods to investigate, and importantly modulate, DNA and RNA triple helix assembly, specificity and molecular interactions. Resulting insights will underpin novel approaches to gene regulation, principally in the context of designing new antibacterial agents to address the antibacterial resistance problem.Read moreRead less
Structural and molecular studies of endocrine disruption in Australia fauna. Contamination of waterways with compounds that disrupt hormone (endocrine) function is a major environmental problem and threat to the health and fertility of animals. Specifically, we lack an understanding of how these potent endocrine disrupting compounds function in native species. Using an innovative combination of structural and molecular biology approaches we will elucidate the mechanisms of action of environmenta ....Structural and molecular studies of endocrine disruption in Australia fauna. Contamination of waterways with compounds that disrupt hormone (endocrine) function is a major environmental problem and threat to the health and fertility of animals. Specifically, we lack an understanding of how these potent endocrine disrupting compounds function in native species. Using an innovative combination of structural and molecular biology approaches we will elucidate the mechanisms of action of environmental endocrine disrupting compounds in native aquatic species - model fish and the platypus; and develop novel technologies for their detection. This work will provide an understanding of the environmental threat of these pollutants to our unique wildlife and will guide future waterway management. Read moreRead less
Evolution and function of mammalian sex chromosomes. Research on iconic Australian mammals has profoundly reshaped our understanding of reproductive biology and sex chromosome evolution. In this project we combine unique expertise, international collaboration and novel genetic information about Australia's unique egg-laying mammals (echidna and platypus) to investigate major aspects of reproduction. This work will address fundamental aspects of sex chromosome biology and advance our understandin ....Evolution and function of mammalian sex chromosomes. Research on iconic Australian mammals has profoundly reshaped our understanding of reproductive biology and sex chromosome evolution. In this project we combine unique expertise, international collaboration and novel genetic information about Australia's unique egg-laying mammals (echidna and platypus) to investigate major aspects of reproduction. This work will address fundamental aspects of sex chromosome biology and advance our understanding of mammalian reproduction. The knowledge gained will have application in captive breeding and conservation of these extraordinary Australian mammals. The project also provides opportunity to train research students in cutting edge molecular biology and informatics.Read moreRead less
Nanoparticle regulation of DNA replication and repair pathways. This project aims to understand how physical and chemical properties of nanoparticles influence how cells respond with respect to maintenance of their DNA integrity. It will use an innovative set of cross-disciplinary methodologies to link what nanoparticle parameters can lead to a benign fate in the environment. The project expects to generate new knowledge of how our ecosystem can be fundamentally impacted by nanoscale materials. ....Nanoparticle regulation of DNA replication and repair pathways. This project aims to understand how physical and chemical properties of nanoparticles influence how cells respond with respect to maintenance of their DNA integrity. It will use an innovative set of cross-disciplinary methodologies to link what nanoparticle parameters can lead to a benign fate in the environment. The project expects to generate new knowledge of how our ecosystem can be fundamentally impacted by nanoscale materials. The intended outcome of the project is to position Australia as a world leader in the manufacture of environmentally benign, advanced nano-scale materials. This is expected to provide economic benefits for Australian manufacturing while ensuring preservation of environmental health.Read moreRead less
Saving seagrass from climate change. This research aims to test whether seagrass ecosystems can be safeguarded from climate change impacts by enhancing genetic connectivity in range edge populations using novel genetic rescue approaches. We will use the range edge seagrass meadows of the UNESCO World Heritage Site of Shark Bay as our model, which was significantly impacted by a marine heat wave in 2010/2011. The project will generate new knowledge on how seagrasses can adapt and survive in situ. ....Saving seagrass from climate change. This research aims to test whether seagrass ecosystems can be safeguarded from climate change impacts by enhancing genetic connectivity in range edge populations using novel genetic rescue approaches. We will use the range edge seagrass meadows of the UNESCO World Heritage Site of Shark Bay as our model, which was significantly impacted by a marine heat wave in 2010/2011. The project will generate new knowledge on how seagrasses can adapt and survive in situ. Expected outcomes are improved conservation, management and restoration practices for seagrass meadows. This should provide significant benefits for long-term resilience of this economically and culturally significant ecosystem.Read moreRead less
The evolutionary potential of fragmented and declining populations. This project aims to integrate adaptive genomic and epigenomic information from wild, captive and reintroduced populations to identify evolutionary potential across different life-histories and levels of habitat fragmentation. The project will capitalise on knowledge and genomic resources for Australian freshwater fishes, including a natural experiment of evolution. It is expected that the project will address fundamental and ap ....The evolutionary potential of fragmented and declining populations. This project aims to integrate adaptive genomic and epigenomic information from wild, captive and reintroduced populations to identify evolutionary potential across different life-histories and levels of habitat fragmentation. The project will capitalise on knowledge and genomic resources for Australian freshwater fishes, including a natural experiment of evolution. It is expected that the project will address fundamental and applied questions about the adaptive capacity of populations in their natural environment. The outcomes of the project will help evaluate and improve local and ecosystem-level initiatives towards the sustainable management of aquatic biodiversity impacted by human activities. The project will also inform on management of water resources in the Murray-Darling Basin.Read moreRead less